Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,021,709	T→C	V69A (GTG→GCG)	fliO →	flagellar biosynthesis protein FliO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,021,709	0	T	C	100.0%	204.4 / NA	58	V69A (GTG→GCG)	fliO	flagellar biosynthesis protein FliO
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/31); total (27/31)

CGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGTGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACGGAAGAGATACCGCAGACCGATTTTCAGTCGGTCATGAAAAATTT  >  NC_000913/2021572‑2021844

cgcACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTgcgg                                                                                                                                       >  1:76961/1‑140 (MQ=255)

cgcACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTgcgg                                                                                                                                       >  1:370668/1‑140 (MQ=255)

   aCTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGcggtgg                                                                                                                                    <  2:370668/140‑1 (MQ=255)

    cTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCggtggt                                                                                                                                   <  1:39125/140‑1 (MQ=255)

    cTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCggtggt                                                                                                                                   <  2:1209367/140‑1 (MQ=255)

      cATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCg                                                                                                                                 <  2:973403/140‑1 (MQ=255)

         cGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGAtg                                                                                                                              <  1:228448/140‑1 (MQ=255)

          gCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGAtgt                                                                                                                             >  2:1134196/1‑140 (MQ=255)

             attattGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGa                                                                                                                          <  1:212716/140‑1 (MQ=255)

             attattGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGa                                                                                                                          <  2:76961/140‑1 (MQ=255)

               tattGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAg                                                                                                                        >  2:301267/1‑140 (MQ=255)

               tattGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAg                                                                                                                        >  2:201755/1‑140 (MQ=255)

                 ttGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGAt                                                                                                                      >  1:28732/1‑140 (MQ=255)

                         aTCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCt                                                                                                              <  1:252940/140‑1 (MQ=255)

                         aTCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCt                                                                                                              >  1:483575/1‑140 (MQ=255)

                         aTCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCt                                                                                                              >  1:466923/1‑140 (MQ=255)

                         aTCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCt                                                                                                              >  2:1239451/1‑140 (MQ=255)

                         aTCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCt                                                                                                              <  1:35967/140‑1 (MQ=255)

                         aTCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCt                                                                                                              >  1:229194/1‑140 (MQ=255)

                         aTCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCt                                                                                                              >  1:231669/1‑140 (MQ=255)

                         aTCCTCGCTGCTGCCAGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGc                                                                                                               <  2:1045014/139‑1 (MQ=255)

                         aTCCTCGCTGCTGCCAGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGc                                                                                                               >  1:1045014/1‑139 (MQ=255)

                           ccTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCACGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTgg                                                                                                            >  1:376960/1‑140 (MQ=255)

                             tCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTg                                                                                                          <  1:379618/140‑1 (MQ=255)

                               gctgctGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCt                                                                                                        <  2:466923/140‑1 (MQ=255)

                                ctgctgCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTc                                                                                                       <  2:435454/140‑1 (MQ=255)

                                ctgctgCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTc                                                                                                       <  1:517541/140‑1 (MQ=255)

                                   ctgcCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGc                                                                                                    >  1:497797/1‑140 (MQ=255)

                                              gTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAgg                                                                                         <  1:1134196/140‑1 (MQ=255)

                                                aaaaCGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTc                                                                                       >  2:778886/1‑140 (MQ=255)

                                                   aCGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCaaa                                                                                    <  1:424635/140‑1 (MQ=255)

                                                      gTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAaatca                                                                                 <  1:1239451/140‑1 (MQ=255)

                                                        tGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAaatcaat                                                                               >  1:71148/1‑140 (MQ=255)

                                                        tGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAaatcaat                                                                               >  1:628684/1‑140 (MQ=255)

                                                         gggATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGt                                                                                        <  1:24890/130‑1 (MQ=255)

                                                         gggATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGt                                                                                        >  2:24890/1‑130 (MQ=255)

                                                                gCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCa                                                                       <  2:628684/140‑1 (MQ=255)

                                                                    cTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATaaa                                                                   <  2:710722/140‑1 (MQ=255)

                                                                         cGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTcc                                                              <  2:570554/140‑1 (MQ=255)

                                                                           cACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTcccc                                                            >  1:985812/1‑140 (MQ=255)

                                                                           cACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTcccc                                                            >  2:798081/1‑140 (MQ=255)

                                                                            aCTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTccccc                                                           >  2:461572/1‑140 (MQ=255)

                                                                                gCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCt                                                       >  2:814238/1‑140 (MQ=255)

                                                                                  gTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGc                                                     <  1:814238/140‑1 (MQ=255)

                                                                                        ggTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAAc                                               <  2:995156/140‑1 (MQ=255)

                                                                                        ggTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAAc                                               <  1:634349/140‑1 (MQ=255)

                                                                                          tCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACgg                                             >  1:209562/1‑140 (MQ=255)

                                                                                           cTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACGGa                                            <  2:71148/140‑1 (MQ=255)

                                                                                           cTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGATTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACGGa                                            <  1:378949/140‑1 (MQ=255)

                                                                                                   tAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACgg                                             <  2:253845/131‑1 (MQ=255)

                                                                                                   tAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACgg                                             >  1:253845/1‑131 (MQ=255)

                                                                                                        ccAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACGGAAGAGATACCGCAg                               >  1:1206494/1‑140 (MQ=255)

                                                                                                          aGTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACGGAAGAGATACCGCAGAc                             <  1:461572/140‑1 (MQ=255)

                                                                                                           gTGCTTCACTGGGCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACGGAAGAGATACCGCAGAcc                            <  2:229194/140‑1 (MQ=255)

                                                                                                                     gggCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACGGAAGAGATACCGCAGACCGATTTTCaat                  <  2:1206131/140‑3 (MQ=255)

                                                                                                                     gggCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACGGAAGAGATACCGCAGACCGATTTTCAGt                  <  2:497797/140‑1 (MQ=255)

                                                                                                                     gggCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACGGAAGAGATACCGCAGACCGATTTTCAGt                  <  1:201755/140‑1 (MQ=255)

                                                                                                                     gggCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACGGAAGAGATACCGCAGACCGATTTTCAGt                  <  2:28732/140‑1 (MQ=255)

                                                                                                                      ggCGCGCGTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACGGAAGAGATACCGCAGACCGATTTTCAGTc                 >  2:35943/1‑140 (MQ=255)

                                                                                                                         gcgcgTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACGGAAGAGATACCGCAGACCGATTTTc                     <  2:158606/133‑1 (MQ=255)

                                                                                                                         gcgcgTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACGGAAGAGATACCGCAGACCGATTTTc                     >  1:158606/1‑133 (MQ=255)

                                                                                                                            cgTGAAAGGGTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACGGAAGAGATACCGCAGACCGATTTTCAGTCGGTCAt           >  2:1066625/1‑140 (MQ=255)

                                                                                                                                     gTTGCGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACGGAAGAGATACCGCAGACCGATTTTCAGTCGGTCATGAAAAAttt  >  2:891469/1‑140 (MQ=255)

CGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGGCGCGCGTGAAAGGGTTGTGGTGGTCGATGTGGAAGATGCACGGCTGGTGCTCGGCGTTACCGCAGGTCAAATCAATCTGCTGCATAAACTTCCCCCTTCTGCACCAACGGAAGAGATACCGCAGACCGATTTTCAGTCGGTCATGAAAAATTT  >  NC_000913/2021572‑2021844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A