Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,055,057	A→G	intergenic (+98/‑4)	shiA → / → amn	shikimate:H(+) symporter/AMP nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,055,057	0	A	G	100.0%	159.9 / NA	45	intergenic (+98/‑4)	shiA/amn	shikimate:H(+) symporter/AMP nucleosidase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/22); total (23/22)

TGACCGCTTTGTTGATGAAAGACAGTCAACGCGCTTGATAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGTGGCGAATTACCTGAT  >  NC_000913/2054922‑2055189

tGACCGCTTTGTTGATGAAAGACAGTCAACGCGCTTGATAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGAcgcaca                                                                                                                                  >  2:186419/1‑140 (MQ=255)

tGACCGCTTTGTTGATGAAAGACAGTCAACGCGCTTGATAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGAcgcaca                                                                                                                                  <  1:186419/140‑1 (MQ=255)

                     aCAGTCAACGCGCTTGATAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTc                                                                                                             >  1:855333/1‑140 (MQ=255)

                     aCAGTCAACGCGCTTGATAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTc                                                                                                             >  2:869541/1‑140 (MQ=255)

                      cAGTCAACGCGCTTGATAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCt                                                                                                            <  2:732554/140‑1 (MQ=255)

                        gTCAACGCGCTTGATAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGa                                                                                                          >  2:492458/1‑140 (MQ=255)

                         tCAACGCGCTTGATAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGAc                                                                                                         <  2:855333/140‑1 (MQ=255)

                         tCAACGCGCTTGATAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGAc                                                                                                         >  1:598511/1‑140 (MQ=255)

                          cAACGCGCTTGATAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGAcc                                                                                                        <  1:111346/140‑1 (MQ=255)

                                              cGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATaa                                                                                    >  1:1202528/1‑140 (MQ=255)

                                                      atcCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGAcg                                                                            <  1:567637/140‑1 (MQ=255)

                                                       tcCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCa                                                                                            >  1:680770/1‑123 (MQ=255)

                                                       tcCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCa                                                                                            <  2:680770/123‑1 (MQ=255)

                                                       tcCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGAcgc                                                                           <  2:450869/140‑1 (MQ=255)

                                                       tcCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGAcgc                                                                           >  2:868358/1‑140 (MQ=255)

                                                       tcCGATCTTCGCCTTACACCTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGAcgc                                                                           >  1:43098/1‑140 (MQ=255)

                                                        ccGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGAcgcg                                                                          <  1:662431/140‑1 (MQ=255)

                                                        ccGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGAcgcg                                                                          <  1:1139332/140‑1 (MQ=255)

                                                         cGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACgcgc                                                                         <  1:492458/140‑1 (MQ=255)

                                                           aTCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGAcg                                                                            <  1:97788/135‑1 (MQ=255)

                                                           aTCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGAcg                                                                            >  2:97788/1‑135 (MQ=255)

                                                             cTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACgcgc                                                                         >  2:652472/1‑136 (MQ=255)

                                                             cTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACgcgc                                                                         <  1:652472/136‑1 (MQ=255)

                                                                 gCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAg                                                                 <  1:869541/140‑1 (MQ=255)

                                                                 gCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAg                                                                 <  1:557554/140‑1 (MQ=255)

                                                                      acacTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAg                                                                 >  1:475721/1‑135 (MQ=255)

                                                                      acacTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAg                                                                 <  2:475721/135‑1 (MQ=255)

                                                                      acacTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATc                                                            >  1:1225036/1‑140 (MQ=255)

                                                                          ttttGTTTCACATTTCTATGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTa                                                        >  1:9619/1‑140 (MQ=255)

                                                                           tttGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAg                                                       <  1:382671/140‑1 (MQ=255)

                                                                               tttCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGc                                                   >  2:279075/1‑140 (MQ=255)

                                                                                 tCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCAt                                                 >  1:172350/1‑140 (MQ=255)

                                                                                        tCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCaa                                          >  2:1083377/1‑140 (MQ=255)

                                                                                             gACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCa                                     <  1:1083377/140‑1 (MQ=255)

                                                                                                  aCTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGc                                <  2:143855/140‑1 (MQ=255)

                                                                                                  aCTATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGc                                >  1:796543/1‑140 (MQ=255)

                                                                                                    tATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCaa                              <  1:868358/140‑1 (MQ=255)

                                                                                                    tATCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACGCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCaa                              <  1:721835/140‑1 (MQ=255)

                                                                                                     aTCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAAc                             <  2:1225036/140‑1 (MQ=255)

                                                                                                      tCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCa                               <  2:293829/137‑1 (MQ=255)

                                                                                                      tCGGATGTGCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCa                               >  1:293829/1‑137 (MQ=255)

                                                                                                           tgtgCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCaa                              <  2:36385/133‑1 (MQ=255)

                                                                                                           tgtgCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCaa                              >  1:36385/1‑133 (MQ=255)

                                                                                                             tgCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTAc                     >  2:228270/1‑140 (MQ=255)

                                                                                                             tgCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTAc                     >  1:1005190/1‑140 (MQ=255)

                                                                                                              gCGGTAATTGTATGGAACAGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACa                    >  2:1070402/1‑140 (MQ=255)

                                                                                                                                aGGAGACGCACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGTGGCGAATTACCtgat  >  2:878330/1‑140 (MQ=255)

TGACCGCTTTGTTGATGAAAGACAGTCAACGCGCTTGATAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGTGGCGAATTACCTGAT  >  NC_000913/2054922‑2055189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A