Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,110,445	G→A	P192L (CCG→CTG)	rfbA ←	dTDP‑glucose pyrophosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,110,445	0	G	A	100.0%	213.6 / NA	60	P192L (CCG→CTG)	rfbA	dTDP‑glucose pyrophosphorylase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (32/28); total (32/28)

CAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACA  >  NC_000913/2110321‑2110579

cAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCg                                                                                                                         <  2:525784/140‑1 (MQ=255)

cAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCg                                                                                                                         <  2:984737/140‑1 (MQ=255)

   aCTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCa                                                                                                                      >  1:1182750/1‑140 (MQ=255)

    ctctGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCAt                                                                                                                     >  2:604269/1‑140 (MQ=255)

    ctctGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCAt                                                                                                                     >  2:792725/1‑140 (MQ=255)

    ctctGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCAt                                                                                                                     >  2:74527/1‑140 (MQ=255)

     tctGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATc                                                                                                                    >  1:512626/1‑140 (MQ=255)

         atgaGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCg                                                                                                                         <  1:1049513/131‑1 (MQ=255)

         atgaGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCg                                                                                                                         >  2:1049513/1‑131 (MQ=255)

         atgaGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGaa                                                                                                                >  1:207966/1‑140 (MQ=255)

         atgaGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGaa                                                                                                                >  2:1256233/1‑140 (MQ=255)

             gTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCg                                                                                                                         >  1:63118/1‑127 (MQ=255)

             gTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCg                                                                                                                         <  2:63118/127‑1 (MQ=255)

             gTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCAc                                                                                                            >  1:1096860/1‑140 (MQ=255)

             gTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCAc                                                                                                            >  2:809110/1‑140 (MQ=255)

              tCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCAcg                                                                                                           >  2:180409/1‑140 (MQ=255)

                 ccGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCAcgtcg                                                                                                        <  2:470133/140‑1 (MQ=255)

                     gtCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTAt                                                                                                    <  1:1249531/140‑1 (MQ=255)

                     gtCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTAt                                                                                                    <  1:711354/140‑1 (MQ=255)

                          gCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATaa                                                                                               <  1:180409/140‑1 (MQ=255)

                          gCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATaa                                                                                               <  2:1096860/140‑1 (MQ=255)

                           ccACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATaaa                                                                                              >  2:1241224/1‑140 (MQ=255)

                                cgTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTAc                                                                                         <  1:1099059/140‑1 (MQ=255)

                                cgTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTAc                                                                                         <  1:958234/140‑1 (MQ=255)

                                   aGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGa                                                                                      >  1:445076/1‑140 (MQ=255)

                                             catcatCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACgg                                                                            <  2:579561/140‑1 (MQ=255)

                                             catcatCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACgg                                                                            <  2:445076/140‑1 (MQ=255)

                                             catcatCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACgg                                                                            <  1:711312/140‑1 (MQ=255)

                                             catcatCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACgg                                                                            <  1:302950/140‑1 (MQ=255)

                                               tcatcGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACATACCTGTAACGGCg                                                                          >  1:147858/1‑140 (MQ=255)

                                                catcGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGt                                                                         <  1:814466/140‑1 (MQ=255)

                                                   cgcgACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAAt                                                                      <  1:809110/140‑1 (MQ=255)

                                                     cgACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTa                                                                    >  1:485291/1‑140 (MQ=255)

                                                            cagacGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTg                                                             <  1:1256233/140‑1 (MQ=255)

                                                                    ccTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAAc                                                     >  2:1154240/1‑140 (MQ=255)

                                                                    ccTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAAc                                                     >  1:261229/1‑140 (MQ=255)

                                                                         tCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTg                                                             >  2:1090893/1‑127 (MQ=255)

                                                                         tCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTg                                                             <  1:1090893/127‑1 (MQ=255)

                                                                         tCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGttt                                                <  2:922499/140‑1 (MQ=255)

                                                                         tCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGttt                                                <  2:485291/140‑1 (MQ=255)

                                                                           aaGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGttttt                                              >  1:43424/1‑140 (MQ=255)

                                                                             gATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTttct                                            >  2:632760/1‑140 (MQ=255)

                                                                                aaaTACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTcc                                         <  2:1202544/140‑1 (MQ=255)

                                                                                   tACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGa                                      >  2:100835/1‑140 (MQ=255)

                                                                                     cGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACt                                    >  1:354548/1‑140 (MQ=255)

                                                                                       gTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGa                                  >  1:646983/1‑140 (MQ=255)

                                                                                       gTTAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGa                                  >  1:998365/1‑140 (MQ=255)

                                                                                        ttAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACt                                    <  1:1011238/137‑1 (MQ=255)

                                                                                        ttAATATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACt                                    >  2:1011238/1‑137 (MQ=255)

                                                                                          aaTATCTGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTg                               <  2:998365/140‑1 (MQ=255)

                                                                                                tGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTAc                         >  2:4241/1‑140 (MQ=255)

                                                                                                tGTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTAc                         >  2:680144/1‑140 (MQ=255)

                                                                                                tGTAATTTCTAACTCACCACGCGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTAc                         >  2:1250102/1‑140 (MQ=255)

                                                                                                 gTAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTAcc                        >  2:525611/1‑140 (MQ=255)

                                                                                                  tAATTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCg                       >  1:33900/1‑140 (MQ=255)

                                                                                                   aaTTTCTAACTCACCACGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGt                      <  2:1039387/140‑1 (MQ=255)

                                                                                                              caccacGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAAc           <  2:207966/140‑1 (MQ=255)

                                                                                                                ccacGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTc         >  1:550160/1‑140 (MQ=255)

                                                                                                                  acGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCaa       <  2:646983/140‑1 (MQ=255)

                                                                                                                  acGTGCAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCaa       <  2:14671/140‑1 (MQ=255)

                                                                                                                       cAGACAGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGaca  >  2:1189389/1‑140 (MQ=255)

CAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACA  >  NC_000913/2110321‑2110579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A