Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,175,780	G→A	A181V (GCC→GTC)	gatZ ←	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,175,780	0	G	A	100.0%	235.2 / NA	67	A181V (GCC→GTC)	gatZ	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatZ
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (40/27); total (40/27)

ATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAA  >  NC_000913/2175643‑2175916

aTTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTTACTGAATGacg                                                                                                                                        >  1:579120/1‑140 (MQ=255)

  ttcacaCCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCt                                                                                                                                      <  2:500837/138‑1 (MQ=255)

     acacCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGc                                                                                                                                   >  2:1007790/1‑140 (MQ=255)

      cacCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGcc                                                                                                                                  >  2:18754/1‑140 (MQ=255)

        cccGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTc                                                                                                                                >  2:578770/1‑140 (MQ=255)

           ggCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCAcc                                                                                                                             >  1:733041/1‑140 (MQ=255)

            gCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCg                                                                                                                            >  1:213117/1‑140 (MQ=255)

            gCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCg                                                                                                                            >  2:805478/1‑140 (MQ=255)

              tGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGcc                                                                                                                          <  1:889821/140‑1 (MQ=255)

               gCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGccc                                                                                                                         <  2:422848/140‑1 (MQ=255)

                         ggCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCccggaaccggaa                                                                                                               <  2:331593/140‑1 (MQ=255)

                              tCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTTACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCg                                                                                                          <  2:579120/140‑1 (MQ=255)

                                acacGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGt                                                                                                        <  2:995319/140‑1 (MQ=255)

                                acacGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGt                                                                                                        <  2:296359/140‑1 (MQ=255)

                                acacGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGt                                                                                                        <  1:957927/140‑1 (MQ=255)

                                     tgtTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCaa                                                                                                   >  1:165438/1‑140 (MQ=255)

                                     tgtTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCaa                                                                                                   >  1:1236288/1‑140 (MQ=255)

                                     tgtTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCaa                                                                                                   <  1:465823/140‑1 (MQ=255)

                                      gtTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAAt                                                                                                  >  2:345259/1‑140 (MQ=255)

                                        tAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGa                                                                                                >  1:30054/1‑140 (MQ=255)

                                        tAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGa                                                                                                <  2:733041/140‑1 (MQ=255)

                                            gCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATa                                                                                            >  2:956198/1‑140 (MQ=255)

                                              ctctGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCgg                                                                                                         <  2:125054/125‑1 (MQ=255)

                                              ctctGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCgg                                                                                                         >  1:125054/1‑125 (MQ=255)

                                              ctctGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGc                                                                                          >  1:818156/1‑140 (MQ=255)

                                               tctGTCAGCCCACGGGCAATAAA‑GCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTc                                                                                        >  1:818501/1‑140 (MQ=255)

                                                ctGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTc                                                                                        >  2:505627/1‑140 (MQ=255)

                                                ctGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTc                                                                                        >  2:414851/1‑140 (MQ=255)

                                                      gCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGc                                                                                  >  2:287283/1‑140 (MQ=255)

                                                                    aaGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATc                                                                    <  1:956198/140‑1 (MQ=255)

                                                                    aaGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATc                                                                    <  2:1236288/140‑1 (MQ=255)

                                                                    aaGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATc                                                                    <  2:165438/140‑1 (MQ=255)

                                                                        ccTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTc                                                                <  1:805478/140‑1 (MQ=255)

                                                                        ccTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTc                                                                >  1:651518/1‑140 (MQ=255)

                                                                         cTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCg                                                               >  1:127632/1‑140 (MQ=255)

                                                                                   gtacgtaAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTc                                                     >  1:902780/1‑140 (MQ=255)

                                                                                   gtacgtaAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTc                                                     <  1:581625/140‑1 (MQ=255)

                                                                                               ttGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCagcaaagca                                         >  2:39249/1‑140 (MQ=255)

                                                                                               ttGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCagcaaagca                                         >  1:505280/1‑140 (MQ=255)

                                                                                               ttGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCagcaaagca                                         >  1:14496/1‑140 (MQ=255)

                                                                                                tGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCagcaaagcaa                                        >  1:500619/1‑140 (MQ=255)

                                                                                                tGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCagcaaagcaa                                        >  1:401838/1‑140 (MQ=255)

                                                                                                 ggcggcATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAgcaaagcaaa                                       <  1:386237/140‑1 (MQ=255)

                                                                                                 ggcggcATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAgcaaagcaaa                                       >  2:91119/1‑140 (MQ=255)

                                                                                                    ggcATCTTCAACATGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAaagcaaagca                                    <  1:481453/140‑1 (MQ=255)

                                                                                                                 tGGGTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTc                       <  2:818156/140‑1 (MQ=255)

                                                                                                                  gggTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTcc                      >  2:77120/1‑140 (MQ=255)

                                                                                                                  gggTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTcc                      >  2:1180625/1‑140 (MQ=255)

                                                                                                                  gggTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTcc                      >  1:1093960/1‑140 (MQ=255)

                                                                                                                  gggTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTcc                      <  1:1007790/140‑1 (MQ=255)

                                                                                                                   ggTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCg                     >  2:1160136/1‑140 (MQ=255)

                                                                                                                    gTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGc                    >  1:62040/1‑140 (MQ=255)

                                                                                                                    gTGATGTGTACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGc                    >  1:850286/1‑140 (MQ=255)

                                                                                                                         gtgtACTGACTGAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCg               >  2:553037/1‑140 (MQ=255)

                                                                                                                             actgactgAATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTc           >  2:494715/1‑140 (MQ=255)

                                                                                                                              ctgactgaATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCt          >  1:950357/1‑140 (MQ=255)

                                                                                                                                 actggATGACGCTGGCCTCACCGCCCGGAACCGGAACTACGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGt       >  2:591745/1‑140 (MQ=255)

                                                                                                                                 actgaATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTg         >  1:663335/1‑138 (MQ=255)

                                                                                                                                 actgaATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTg         <  2:663335/138‑1 (MQ=255)

                                                                                                                                 actgaATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGt       >  2:1143839/1‑140 (MQ=255)

                                                                                                                                 actgaATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGt       >  2:773919/1‑140 (MQ=255)

                                                                                                                                 actgaATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGt       >  2:357948/1‑140 (MQ=255)

                                                                                                                                    gaATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCt    <  2:14496/140‑1 (MQ=255)

                                                                                                                                    gaATGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCt    <  2:950357/140‑1 (MQ=255)

                                                                                                                                     aaTGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTa   <  2:651518/140‑1 (MQ=255)

                                                                                                                                     aaTGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAACTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTa   <  2:897092/140‑1 (MQ=255)

                                                                                                                                      aTGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTaa  <  1:345259/140‑1 (MQ=255)

                                                                                                                                      aTGACGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTaa  <  2:500619/140‑1 (MQ=255)

ATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAA  >  NC_000913/2175643‑2175916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A