Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 260 73.5 2514320 99.3% 2496719 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,281,013 A→G S128G (AGC→GGC)  radD → putative DNA repair helicase RadD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,281,0130AG100.0% 176.7 / NA 50S128G (AGC→GGC) radDputative DNA repair helicase RadD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/19);  total (31/19)

AAAGCGCAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACG  >  NC_000913/2280880‑2281143
                                                                                                                                     |                                                                                                                                  
aaaGCGCAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGt                                                                                                                              <  2:904491/140‑1 (MQ=255)
      cAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGc                                                                                                                        >  2:1023474/1‑140 (MQ=255)
       aaaGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCa                                                                                                                       <  1:1023474/140‑1 (MQ=255)
       aaaGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCa                                                                                                                       >  2:467256/1‑140 (MQ=255)
                             tATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACaaaa                                                                                                 >  1:345705/1‑140 (MQ=255)
                              aTTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAg                                                                                                >  1:136363/1‑140 (MQ=255)
                               tttGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGt                                                                                               >  2:937734/1‑140 (MQ=255)
                               tttGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGt                                                                                               >  1:569788/1‑140 (MQ=255)
                                   gCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAAt                                                                                           <  1:937734/140‑1 (MQ=255)
                                    cAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATc                                                                                          >  1:358923/1‑140 (MQ=255)
                                      gCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATccc                                                                                        <  2:136363/140‑1 (MQ=255)
                                       cGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATcccc                                                                                       >  1:1044194/1‑140 (MQ=255)
                                         tGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCAc                                                                                     >  1:895928/1‑140 (MQ=255)
                                              tCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACg                                                                                <  1:136755/140‑1 (MQ=255)
                                                     cccGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCctgctg                                                                         <  1:467256/140‑1 (MQ=255)
                                                      ccGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTgg                                                                        >  2:723162/1‑140 (MQ=255)
                                                        gTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTgggg                                                                      >  1:1204944/1‑140 (MQ=255)
                                                        gTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTgggg                                                                      >  1:273660/1‑140 (MQ=255)
                                                        gTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTgggg                                                                      >  1:514928/1‑140 (MQ=255)
                                                          aaTCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCt                                                                    <  2:358923/140‑1 (MQ=255)
                                                                gATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACg                                                                                >  1:333721/1‑122 (MQ=255)
                                                                gATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACg                                                                                <  2:333721/122‑1 (MQ=255)
                                                                   gCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCAc                                                           >  1:951492/1‑140 (MQ=255)
                                                                   gCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCAc                                                           <  2:1044194/140‑1 (MQ=255)
                                                                   gCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCAc                                                           <  2:1133679/140‑1 (MQ=255)
                                                                      tttCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGcc                                                        >  1:546108/1‑140 (MQ=255)
                                                                               gAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGAtt                                               >  1:472306/1‑140 (MQ=255)
                                                                                aaTTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTg                                              >  2:358307/1‑140 (MQ=255)
                                                                                   tttCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGc                                           <  2:273660/140‑1 (MQ=255)
                                                                                    ttCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCa                                          <  1:43375/140‑1 (MQ=255)
                                                                                    ttCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCa                                          <  2:514928/140‑1 (MQ=255)
                                                                                        cTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAgg                                      <  2:546108/140‑1 (MQ=255)
                                                                                          gTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCTGCCTTCCGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCt                                    <  2:569788/140‑1 (MQ=255)
                                                                                           ttGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTg                                   >  2:219242/1‑140 (MQ=255)
                                                                                           ttGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTg                                   >  2:513713/1‑140 (MQ=255)
                                                                                                 gTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTTACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTa                             >  1:569550/1‑140 (MQ=255)
                                                                                                 gTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGc                                           >  1:458572/1‑126 (MQ=255)
                                                                                                 gTCGATGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGc                                           <  2:458572/126‑1 (MQ=255)
                                                                                                      tGAATGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGt                        <  2:1228558/140‑1 (MQ=255)
                                                                                                          tGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCa                    <  2:448763/140‑1 (MQ=255)
                                                                                                          tGTCACCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCa                    >  1:448763/1‑140 (MQ=255)
                                                                                                              aCCGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTAt                >  2:856722/1‑140 (MQ=255)
                                                                                                                cGTATTGGTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCa              <  2:115633/140‑1 (MQ=255)
                                                                                                                       gTGACGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATg       >  1:854163/1‑140 (MQ=255)
                                                                                                                          aCGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTa    >  2:456565/1‑140 (MQ=255)
                                                                                                                          aCGATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTa    >  1:790167/1‑140 (MQ=255)
                                                                                                                            gATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTAcg  >  1:1216156/1‑140 (MQ=255)
                                                                                                                            gATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTAcg  >  1:1090588/1‑140 (MQ=255)
                                                                                                                            gATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTAcg  >  2:944687/1‑140 (MQ=255)
                                                                                                                            gATGAAGAGGGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGACTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTAcg  >  2:574234/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                  
AAAGCGCAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACG  >  NC_000913/2280880‑2281143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A