Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,395,029	T→C	L6L (TTA→TTG)	nuoL ←	NADH:quinone oxidoreductase subunit L

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,395,029	0	T	C	100.0%	142.1 / NA	40	L6L (TTA→TTG)	nuoL	NADH:quinone oxidoreductase subunit L
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/25); total (15/25)

ATCAACGCCGATAAAGGCGGTTACCAGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCG  >  NC_000913/2394894‑2395154

aTCAACGCCGATAAAGGCGGTTACCAGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAgg                                                                                                                           <  2:112217/140‑1 (MQ=255)

   aaCGCCGATAAAGGCGGTTACCAGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCaa                                                                                                                        <  2:1029578/140‑1 (MQ=255)

   aaCGCCGATAAAGGCGGTTACCAGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCaa                                                                                                                        <  1:951090/140‑1 (MQ=255)

   aaCGCCGATAAAGGCGGTTACCAGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCaa                                                                                                                        <  1:706284/140‑1 (MQ=255)

     cGCCGATAAAGGCGGTTACCAGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGc                                                                                                                      >  1:907539/1‑140 (MQ=255)

        cGATAAAGGCGGTTACCAGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCaa                                                                                                                        <  2:839495/135‑1 (MQ=255)

        cGATAAAGGCGGTTACCAGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCaa                                                                                                                        >  1:839495/1‑135 (MQ=255)

          aTAAAGGCGGTTACCAGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGtt                                                                                                                 >  1:1255516/1‑140 (MQ=255)

               ggCGGTTACCAGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATcc                                                                                                            <  1:847738/140‑1 (MQ=255)

                gCGGTTACCAGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCcg                                                                                                           <  2:313125/140‑1 (MQ=255)

                gCGGTTACCAGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCcg                                                                                                           <  2:808237/140‑1 (MQ=255)

                gCGGTTACCAGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCcg                                                                                                           <  2:1001758/140‑1 (MQ=255)

                   gTTACCAGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCgcgc                                                                                                        <  1:1251963/140‑1 (MQ=255)

                     tACCAGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCAt                                                                                                      <  1:321067/140‑1 (MQ=255)

                     tACCAGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCAt                                                                                                      <  2:100700/140‑1 (MQ=255)

                        cAGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCAtctc                                                                                                   <  1:787782/140‑1 (MQ=255)

                         aGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGATAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCa                                                                                                  <  2:872919/140‑1 (MQ=255)

                             ccgccAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTa                                                                                              <  1:517834/140‑1 (MQ=255)

                                ccAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATcc                                                                                                            <  2:1004403/123‑1 (MQ=255)

                                ccAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATcc                                                                                                            >  1:1004403/1‑123 (MQ=255)

                                ccAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTg                                                                                           <  2:846835/140‑1 (MQ=255)

                                   ggCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAAt                                                                                        <  2:710729/140‑1 (MQ=255)

                                         caGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCg                                                                                      >  2:207595/1‑136 (MQ=255)

                                         caGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCg                                                                                      <  1:207595/136‑1 (MQ=255)

                                           gagCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTc                                                                                <  2:428839/140‑1 (MQ=255)

                                                            cGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGacgacg                                                               <  1:524240/140‑1 (MQ=255)

                                                                           aGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTgcagcag                                                <  2:1255516/140‑1 (MQ=255)

                                                                                  cgcCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCa                                         >  1:82849/1‑140 (MQ=255)

                                                                                              aaTGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTc                             >  1:445202/1‑140 (MQ=255)

                                                                                                 gCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCt                          >  2:958687/1‑140 (MQ=255)

                                                                                                   cagcagGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTTATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTc                        >  1:985386/1‑140 (MQ=255)

                                                                                                   cagcagGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTc                        >  2:1897/1‑140 (MQ=255)

                                                                                                      cagGACGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTgc                     >  2:311058/1‑140 (MQ=255)

                                                                                                           cGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGccgccg                >  2:489477/1‑140 (MQ=255)

                                                                                                           cGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGccgccg                >  2:1034625/1‑140 (MQ=255)

                                                                                                           cGAAGCCAATCAATGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGccgccg                >  1:1011697/1‑140 (MQ=255)

                                                                                                                   atcaatGGCAAAATAATGGTCAAGGCAGGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGATCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTg        >  1:453018/1‑140 (MQ=255)

                                                                                                                        tGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGc   <  2:445202/140‑1 (MQ=255)

                                                                                                                        tGGCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGc   <  2:1011697/140‑1 (MQ=255)

                                                                                                                         ggCAAAATAATGGTCAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCg  <  1:489477/140‑1 (MQ=255)

ATCAACGCCGATAAAGGCGGTTACCAGCGCCGCCAGGCCCACAGAGCCTACGCCGACGATCGCCGAGACGTTTTCAGACCAGCGCCCACGGGAGAATGCCAGCAGGACGAAGCCAATCAATGGCAAAATAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCG  >  NC_000913/2394894‑2395154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A