Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 260 73.5 2514320 99.3% 2496719 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,410,707 T→C T216A (ACC→GCC)  yfbS ← putative transporter YfbS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,410,7070TC100.0% 202.9 / NA 58T216A (ACC→GCC) yfbSputative transporter YfbS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/32);  total (26/32)

CGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGTTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGC  >  NC_000913/2410572‑2410838
                                                                                                                                       |                                                                                                                                    
cGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGa                                                                                                                                  <  1:1108533/140‑1 (MQ=255)
cGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGa                                                                                                                                  >  2:1108533/1‑140 (MQ=255)
  aTGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGAcg                                                                                                                                >  1:849366/1‑140 (MQ=255)
               cATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTcg                                                                                                                   >  2:969812/1‑140 (MQ=255)
                 tAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTcgcg                                                                                                                 >  2:187926/1‑140 (MQ=255)
                 tAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTcgcg                                                                                                                 >  1:786032/1‑140 (MQ=255)
                      cTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑g                                                                                                           <  2:246209/140‑1 (MQ=255)
                       tCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑gg                                                                                                          >  2:563747/1‑140 (MQ=255)
                         aCGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑gggg                                                                                                        <  1:421269/140‑1 (MQ=255)
                              aTTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTgg                                                                                                   <  1:1040050/140‑1 (MQ=255)
                              aTTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTgg                                                                                                   >  2:1040050/1‑140 (MQ=255)
                                         tcCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCttt                                                                                        >  2:504302/1‑140 (MQ=255)
                                         tcCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCttt                                                                                        >  1:950129/1‑140 (MQ=255)
                                                   gACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGaaa                                                                              <  2:849366/140‑1 (MQ=255)
                                                   gACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGaaa                                                                              >  2:828569/1‑140 (MQ=255)
                                                    aCCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAAc                                                                             <  2:173845/140‑1 (MQ=255)
                                                        aTCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCAt                                                                         >  1:655706/1‑140 (MQ=255)
                                                          cATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCa                                                                       <  2:63054/140‑1 (MQ=255)
                                                           aTTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCAc                                                                      >  2:1180309/1‑140 (MQ=255)
                                                            ttGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACt                                                                     <  2:950129/140‑1 (MQ=255)
                                                            ttGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACt                                                                     <  1:1072343/140‑1 (MQ=255)
                                                            ttGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACt                                                                     <  1:1180309/140‑1 (MQ=255)
                                                              ggCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCCCGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTaa                                                                   >  1:996735/1‑140 (MQ=255)
                                                                   tCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCa                                                                          >  1:396412/1‑128 (MQ=255)
                                                                   tCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCa                                                                          <  2:396412/128‑1 (MQ=255)
                                                                   tCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACatat                                                              <  2:2427/140‑1 (MQ=255)
                                                                   tCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACatat                                                              >  1:145972/1‑140 (MQ=255)
                                                                   tCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACatat                                                              <  1:187926/140‑1 (MQ=255)
                                                                       gggCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACat                                                                <  2:468429/134‑1 (MQ=255)
                                                                       gggCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACat                                                                >  1:468429/1‑134 (MQ=255)
                                                                             aTAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAATGCATCACTAACATATACAAAATAcc                                                    <  2:264626/140‑1 (MQ=255)
                                                                             aTAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATccc                                                    <  2:217798/140‑1 (MQ=255)
                                                                             aTAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATAcc                                                    <  2:786032/140‑1 (MQ=255)
                                                                             aTAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATAcc                                                    <  1:987064/140‑1 (MQ=255)
                                                                             aTAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATAcc                                                    >  1:210364/1‑140 (MQ=255)
                                                                             aTAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATAcc                                                    <  1:935696/140‑1 (MQ=255)
                                                                              tAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACt                                                                     >  2:278760/1‑122 (MQ=255)
                                                                              tAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACt                                                                     <  1:278760/122‑1 (MQ=255)
                                                                                 ccAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACccagc                                                <  2:628482/140‑1 (MQ=255)
                                                                                       gTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACccagcaccagc                                          <  2:210364/140‑1 (MQ=255)
                                                                                       gTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACccagcaccagc                                          >  2:80230/1‑140 (MQ=255)
                                                                                           cgcACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGa                                      >  1:180300/1‑140 (MQ=255)
                                                                                            gcACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGAc                                     <  1:984565/140‑1 (MQ=255)
                                                                                             cACGCCCGGTCAGTCGCTATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGCGGGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGAc                                     <  2:1124307/140‑1 (MQ=255)
                                                                                             cACGCCCGGTCAGTCGATATTCCCGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGAcc                                    <  2:996735/140‑1 (MQ=255)
                                                                                             cACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGAcc                                    <  2:180300/140‑1 (MQ=255)
                                                                                               cGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGACCAg                                  >  2:235253/1‑140 (MQ=255)
                                                                                               cGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGACCAg                                  <  2:145972/140‑1 (MQ=255)
                                                                                                gCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGACCAgg                                 <  1:1041306/140‑1 (MQ=255)
                                                                                                  ccGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGAc                                     <  2:10983/134‑1 (MQ=255)
                                                                                                  ccGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGAc                                     >  1:10983/1‑134 (MQ=255)
                                                                                                          tCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGgtg                       >  1:982951/1‑140 (MQ=255)
                                                                                                          tCGATATTCACGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGgtg                       <  1:80230/140‑1 (MQ=255)
                                                                                                                    cGGATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTaaa             >  2:49158/1‑140 (MQ=255)
                                                                                                                      gATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGgtgt                      >  2:753191/1‑129 (MQ=255)
                                                                                                                      gATAAGATCGCGAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGgtgt                      <  1:753191/129‑1 (MQ=255)
                                                                                                                                 gAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGc  <  2:55777/138‑1 (MQ=255)
                                                                                                                                 gAAAGGCTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGc  >  1:55777/1‑138 (MQ=255)
                                                                                                                                       |                                                                                                                                    
CGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATATTCACGGATAAGATCGCGAAAGGTTCGACGCGTCCAGCCTTCGCGCTGC‑GGGGTCTGGGTATCCCCTTTCAGCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGC  >  NC_000913/2410572‑2410838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A