Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,565,398	A→G	intergenic (+66/+83)	yffS → / ← eutA	CPZ‑55 prophage; uncharacterized protein YffS/reactivating factor for ethanolamine ammonia‑lyase EutBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,565,398	0	A	G	100.0%	196.3 / NA	55	intergenic (+66/+83)	yffS/eutA	CPZ‑55 prophage; uncharacterized protein YffS/reactivating factor for ethanolamine ammonia‑lyase EutBC
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (30/25); total (30/25)

GGCAAAGGCAAGAAAGGTGACAAGCTGATAACTTTGCTTCCCAACTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGAT  >  NC_000913/2565261‑2565526

ggCAAAGGCAAGAAAGGTGACAAGCTGATAACTTTGCTTCCCAACTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACcgca                                                                                                                                >  1:1238857/1‑140 (MQ=255)

       gCAAGAAAGGTGACAAGCTGATAACTTTGCTTCCCAACTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACCCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTAccc                                                                                                                         <  2:1117310/140‑1 (MQ=255)

                 tGACAAGCTGATAACTTTGCTTCCCAACTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCa                                                                                                                       >  2:264486/1‑132 (MQ=255)

                 tGACAAGCTGATAACTTTGCTTCCCAACTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCa                                                                                                                       <  1:264486/132‑1 (MQ=255)

                 tGACAAGCTGATAACTTTGCTTCCCAACTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATa                                                                                                               <  1:661437/140‑1 (MQ=255)

                 tGACAAGCTGATAACTTTGCTTCCCAACTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATa                                                                                                               <  2:72132/140‑1 (MQ=255)

                   aCAAGCTGATAACTTTGCTTCCCAACTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATaca                                                                                                             >  2:242958/1‑140 (MQ=255)

                   aCAAGCTGATAACTTTGCTTCCCAACTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATaca                                                                                                             >  2:143227/1‑140 (MQ=255)

                       gCTGATAACTTTGCTTCCCAACTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGc                                                                                                         >  2:58459/1‑140 (MQ=255)

                                 ttGCTTCCCAACTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAAt                                                                                               <  1:242958/140‑1 (MQ=255)

                                  tGCTTCCCATCTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAAtt                                                                                              <  2:447920/140‑1 (MQ=255)

                                  tGCTTCCCAACTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATaca                                                                                                             <  2:763533/125‑1 (MQ=255)

                                  tGCTTCCCAACTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATaca                                                                                                             >  1:763533/1‑125 (MQ=255)

                                  tGCTTCCCAACTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAg                                                                                                          >  1:452644/1‑128 (MQ=255)

                                  tGCTTCCCAACTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAg                                                                                                          <  2:452644/128‑1 (MQ=255)

                                  tGCTTCCCAACTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAAtt                                                                                              >  1:805724/1‑140 (MQ=255)

                                  tGCTTCCCAACTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAAtt                                                                                              >  1:910494/1‑140 (MQ=255)

                                          aaCTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGc                                                                                      >  1:722210/1‑140 (MQ=255)

                                               gATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGcaccca                                                                                 >  2:1182200/1‑140 (MQ=255)

                                                 ttGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGcacccagc                                                                               >  2:448923/1‑140 (MQ=255)

                                                         cTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGcacccagccacccagc                                                                       >  1:302080/1‑140 (MQ=255)

                                                           gggAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAgcgc                                                                     >  1:999544/1‑140 (MQ=255)

                                                           gggAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAgcgc                                                                     >  1:1009624/1‑140 (MQ=255)

                                                           gggAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAgcgc                                                                     <  2:292003/140‑1 (MQ=255)

                                                           gggAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAgcgc                                                                     <  1:448923/140‑1 (MQ=255)

                                                           gggAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAgcgc                                                                     <  1:1182200/140‑1 (MQ=255)

                                                            ggAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGcc                                                                    >  1:953648/1‑140 (MQ=255)

                                                              agaCGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCAc                                                                  <  2:1009624/140‑1 (MQ=255)

                                                                aCGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCg                                                                >  1:1125835/1‑140 (MQ=255)

                                                                aCGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCg                                                                <  1:143227/140‑1 (MQ=255)

                                                                       aaCCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGa                                                         <  2:594330/140‑1 (MQ=255)

                                                                            gCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATAcc                                                    >  2:976442/1‑140 (MQ=255)

                                                                             ccTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCAc                                                                  >  1:691660/1‑125 (MQ=255)

                                                                             ccTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCAc                                                                  <  2:691660/125‑1 (MQ=255)

                                                                             ccTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATa                                                      >  1:782097/1‑137 (MQ=255)

                                                                             ccTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATa                                                      <  2:782097/137‑1 (MQ=255)

                                                                                 cAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCAtt                                               >  1:430642/1‑140 (MQ=255)

                                                                                  aaCCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATa                                                      <  1:747120/132‑1 (MQ=255)

                                                                                  aaCCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATa                                                      >  2:747120/1‑132 (MQ=255)

                                                                                       cATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCaggaa                                         >  1:657364/1‑140 (MQ=255)

                                                                                              tcaatcaaCGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATg                                  >  1:453784/1‑140 (MQ=255)

                                                                                              tcaatcaaCGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATg                                  >  2:690179/1‑140 (MQ=255)

                                                                                              tcaatcaaCGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATg                                  >  1:234393/1‑140 (MQ=255)

                                                                                                     aCGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATGCg                                <  1:799261/135‑1 (MQ=255)

                                                                                                     aCGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATGCg                                >  2:799261/1‑135 (MQ=255)

                                                                                                       gCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATGCGAGTGAtt                         >  1:527057/1‑140 (MQ=255)

                                                                                                       gCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATGCGAGTGAtt                         >  2:731413/1‑140 (MQ=255)

                                                                                                            gCCCCGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTtcacc                    >  2:60342/1‑140 (MQ=255)

                                                                                                               ccGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTtcaccgtc                 <  2:430642/140‑1 (MQ=255)

                                                                                                                cGCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTtcaccgtca                <  1:60342/140‑1 (MQ=255)

                                                                                                                 gCTGCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCACTGGCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTtcaccgtcac               <  2:999544/140‑1 (MQ=255)

                                                                                                                    gCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCgg            <  2:265208/140‑1 (MQ=255)

                                                                                                                    gCCGGGTTTTTGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCgg            <  2:453784/140‑1 (MQ=255)

                                                                                                                              tGCTATGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGAt  <  2:722210/140‑1 (MQ=255)

                                                                                                                                   tGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCgg            <  2:651604/125‑1 (MQ=255)

                                                                                                                                   tGCACCGCAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCgg            >  1:651604/1‑125 (MQ=255)

GGCAAAGGCAAGAAAGGTGACAAGCTGATAACTTTGCTTCCCAACTGGATTGATAAGCTGGGAGACGAATAAACCAGCCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGAT  >  NC_000913/2565261‑2565526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A