Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,709,225	C→T	M60I (ATG→ATA)	rseA ←	anti‑sigma factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,709,225	0	C	T	100.0%	232.0 / NA	67	M60I (ATG→ATA)	rseA	anti‑sigma factor
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (30/37); total (30/37)

ACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCATTTCTGGGTTATGAGCCAGTTCGTTAAGCAGCTCACTATCC  >  NC_000913/2709088‑2709360

aCGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTAt                                                                                                                                       <  2:650028/140‑1 (MQ=255)

aCGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTAt                                                                                                                                       >  1:1161732/1‑140 (MQ=255)

  gCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCa                                                                                                                                     >  1:1090107/1‑140 (MQ=255)

  gCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCa                                                                                                                                     <  2:172553/140‑1 (MQ=255)

  gCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCa                                                                                                                                     <  1:926443/140‑1 (MQ=255)

    ccATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCAcg                                                                                                                                   >  1:4839/1‑140 (MQ=255)

        ttGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTg                                                                                                                               >  2:734744/1‑140 (MQ=255)

        ttGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTg                                                                                                                               <  1:161081/140‑1 (MQ=255)

             tAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAagag                                                                                                                          <  1:1063233/140‑1 (MQ=255)

             tAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAagag                                                                                                                          <  1:506402/140‑1 (MQ=255)

             tAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAagag                                                                                                                          <  1:14082/140‑1 (MQ=255)

                 cTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAagag                                                                                                                          <  2:498116/136‑1 (MQ=255)

                 cTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAagag                                                                                                                          >  1:498116/1‑136 (MQ=255)

                 cTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGatat                                                                                                                      >  2:703124/1‑140 (MQ=255)

                 cTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGatat                                                                                                                      <  1:725573/140‑1 (MQ=255)

                  tgtgCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATc                                                                                                                     >  1:602071/1‑140 (MQ=255)

                  tgtgCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATc                                                                                                                     >  1:1100705/1‑140 (MQ=255)

                   gtgCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCg                                                                                                                    <  2:1131120/140‑1 (MQ=255)

                    tgCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGa                                                                                                                   >  1:1099327/1‑140 (MQ=255)

                         cccACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATgg                                                                                                              >  1:760514/1‑140 (MQ=255)

                           cACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCg                                                                                                                    >  1:1109765/1‑132 (MQ=255)

                           cACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAg                                                                                                            <  2:440108/140‑1 (MQ=255)

                           cACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAg                                                                                                            <  1:756294/140‑1 (MQ=255)

                           cACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAg                                                                                                            <  1:734744/140‑1 (MQ=255)

                           cACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAg                                                                                                            <  2:1188664/140‑1 (MQ=255)

                           cACGGACGTACTTTCTGCCAGAATAGCATTTTATGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTATTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCg                                                                                                                    <  2:1109765/132‑1 (MQ=255)

                                aCGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCt                                                                                                       <  2:4839/140‑1 (MQ=255)

                                   tACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCgg                                                                                                    <  2:1100705/140‑1 (MQ=255)

                                   tACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCgg                                                                                                    <  2:1080497/140‑1 (MQ=255)

                                          tGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATc                                                                                             >  2:701433/1‑140 (MQ=255)

                                          tGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATc                                                                                             >  1:965981/1‑140 (MQ=255)

                                          tGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATc                                                                                             >  1:854484/1‑140 (MQ=255)

                                            ccAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCAc                                                                                           <  1:313329/140‑1 (MQ=255)

                                             cAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCAcc                                                                                          <  2:919628/140‑1 (MQ=255)

                                              aGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCAccc                                                                                         <  1:1180924/140‑1 (MQ=255)

                                                    gCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGCCGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCAtt                                                                                   <  2:1099327/140‑1 (MQ=255)

                                                          tCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCa                                                                             <  2:1161732/140‑1 (MQ=255)

                                                          tCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCa                                                                             <  2:965981/140‑1 (MQ=255)

                                                              ccATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGa                                                                         <  1:318085/140‑1 (MQ=255)

                                                                aTTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGAtt                                                                       <  1:701433/140‑1 (MQ=255)

                                                                aTTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGAtt                                                                       <  2:914856/140‑1 (MQ=255)

                                                                  tGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTaa                                                                     >  2:461871/1‑140 (MQ=255)

                                                                          cgcAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGc                                                             >  2:708601/1‑140 (MQ=255)

                                                                                     cTTCCGGGATCGATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTAGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGtt                                                  <  2:545243/140‑1 (MQ=255)

                                                                                         cGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCt                                              <  2:602071/140‑1 (MQ=255)

                                                                                         cGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCt                                              >  2:686051/1‑140 (MQ=255)

                                                                                          gggATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTg                                             <  1:708601/140‑1 (MQ=255)

                                                                                              tCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCAtt                                         <  1:703124/140‑1 (MQ=255)

                                                                                                  tGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTg                                             >  2:1202922/1‑132 (MQ=255)

                                                                                                  tGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTg                                             <  1:1202922/132‑1 (MQ=255)

                                                                                                       ccGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCAtt                                         >  2:343586/1‑131 (MQ=255)

                                                                                                       ccGGTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCAtt                                         <  1:343586/131‑1 (MQ=255)

                                                                                                          gTTGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCATTTCTGGGTTATGa                             >  1:1001136/1‑140 (MQ=255)

                                                                                                            tGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCATTTCTg                                     <  1:727475/130‑1 (MQ=255)

                                                                                                            tGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCATTTCTg                                     >  2:727475/1‑130 (MQ=255)

                                                                                                            tGACGTACTGGCTCTTCTTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCATTTCTGGGTTATGAGc                           >  1:806078/1‑140 (MQ=255)

                                                                                                                        tcttctTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCATTTCTGGGTTATGAGCCAGtt                      <  2:158995/133‑1 (MQ=255)

                                                                                                                        tcttctTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCATTTCTGGGTTATGAGCCAGtt                      >  1:158995/1‑133 (MQ=255)

                                                                                                                        tcttctTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCATTTCTGGGTTATGAGCCAGTTCGTTAag               <  2:856966/140‑1 (MQ=255)

                                                                                                                        tcttctTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCATTTCTGGGTTATGAGCCAGTTCGTTAag               >  1:856966/1‑140 (MQ=255)

                                                                                                                        tcttctTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCATTTCTGGGTTATGAGCCAGTTCGTTAag               <  1:1127757/140‑1 (MQ=255)

                                                                                                                        tcttctTCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCATTTCTGGGTTATGAGCCAGTTCGTTAag               >  2:171319/1‑140 (MQ=255)

                                                                                                                         cttcttCAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCATTTCTGGGTTATGAGCCAGTTCGTTAagc              >  1:1174856/1‑140 (MQ=255)

                                                                                                                           tcttcAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCATTTCTGGGTTATGAGCCAGTTCGTTAagcag            >  2:178732/1‑140 (MQ=255)

                                                                                                                           tcttcAATGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCATTTCTGGGTTATGAGCCAGTTCGTTAagcag            >  1:1197113/1‑140 (MQ=255)

                                                                                                                                  tGGCGGCTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCATTTCTGGGTTATGAGCCAGTTCGTTAAGCAGCTCACTa     >  2:802464/1‑140 (MQ=255)

                                                                                                                                     cggcTATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCATTTCTGGGTTATGAGCCAGTTCGTTAAGCAGCTCACTATcc  >  1:954272/1‑140 (MQ=255)

ACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCATTTCTGGGTTATGAGCCAGTTCGTTAAGCAGCTCACTATCC  >  NC_000913/2709088‑2709360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A