Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 260 73.5 2514320 99.3% 2496719 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,746,240 T→C intergenic (‑55/+194) rpsP ← / ← ffh 30S ribosomal subunit protein S16/signal recognition particle protein component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,746,2400TC98.6% 249.9 / ‑6.1 71intergenic (‑55/+194)rpsP/ffh30S ribosomal subunit protein S16/signal recognition particle protein component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (41/29);  minor base G (1/0);  total (42/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGTCACCGGAGCGTACACGC  >  NC_000913/2746112‑2746376
                                                                                                                                |                                                                                                                                        
cGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTaaa                                                                                                                               <  1:16007/140‑1 (MQ=255)
      tCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCg                                                                                                                         >  1:656549/1‑140 (MQ=255)
          cGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGaaaa                                                                                                                     >  1:396013/1‑140 (MQ=255)
          cGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGaaaa                                                                                                                     >  1:1250291/1‑140 (MQ=255)
                       gTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCAtttt                                                                                                        >  1:207401/1‑140 (MQ=255)
                          gAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGc                                                                                                     <  2:656549/140‑1 (MQ=255)
                            aCGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCgg                                                                                                   <  1:926579/140‑1 (MQ=255)
                             cGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCggg                                                                                                  >  1:1046497/1‑140 (MQ=255)
                             cGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCggg                                                                                                  >  1:882492/1‑140 (MQ=255)
                             cGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCggg                                                                                                  >  1:523013/1‑140 (MQ=255)
                             cGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCggg                                                                                                  >  1:1103296/1‑140 (MQ=255)
                                aCGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAAt                                                                                               >  1:350397/1‑140 (MQ=255)
                                  gCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTg                                                                                             >  1:972762/1‑140 (MQ=255)
                                  gCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTg                                                                                             >  2:814480/1‑140 (MQ=255)
                                     ttttAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAA‑‑‑‑CGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCaa                                                                                      <  2:598250/140‑1 (MQ=255)
                                         aGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCaa                                                                                      >  2:823601/1‑140 (MQ=255)
                                           cgcCGTGACGTGCTTAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACa                                                                                    <  2:288340/140‑1 (MQ=255)
                                           cgcCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACa                                                                                    <  2:396013/140‑1 (MQ=255)
                                            gcCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAg                                                                                   <  2:1250291/140‑1 (MQ=255)
                                                tGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACa                                                                                    >  1:435479/1‑135 (MQ=255)
                                                tGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACa                                                                                    <  2:435479/135‑1 (MQ=255)
                                                tGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGc                                                                               >  1:262727/1‑140 (MQ=255)
                                                tGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGc                                                                               >  1:470749/1‑140 (MQ=255)
                                                 gACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCt                                                                              <  2:882492/140‑1 (MQ=255)
                                                 gACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCt                                                                              <  2:262727/140‑1 (MQ=255)
                                                   cGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTaa                                                                            <  2:796179/140‑1 (MQ=255)
                                                      gCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAActc                                                                         <  2:470749/140‑1 (MQ=255)
                                                               aTAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTaaa                                                                >  2:363660/1‑140 (MQ=255)
                                                               aTAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTaaa                                                                >  2:896050/1‑140 (MQ=255)
                                                                     aCCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAAct                                                                          >  1:904543/1‑124 (MQ=255)
                                                                     aCCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAAct                                                                          <  2:904543/124‑1 (MQ=255)
                                                                      ccATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGt                                                         <  2:207401/140‑1 (MQ=255)
                                                                        aTAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTcg                                                       >  1:945270/1‑140 (MQ=255)
                                                                           aCATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTcggcg                                                    >  2:75798/1‑140 (MQ=255)
                                                                             aTCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGt                                                  <  2:523013/140‑1 (MQ=255)
                                                                               ccTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGc                                                <  2:743362/140‑1 (MQ=255)
                                                                               ccTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGc                                                >  1:1036847/1‑140 (MQ=255)
                                                                               ccTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGc                                                >  1:743362/1‑140 (MQ=255)
                                                                                 tctTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAg                                              >  1:804764/1‑140 (MQ=255)
                                                                                   ttGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGGGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCATCAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGcc                                            >  2:1172286/1‑140 (MQ=255)
                                                                                      tgtgAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGt                                         >  2:1197252/1‑140 (MQ=255)
                                                                                       gtgAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGtt                                        <  2:408668/140‑1 (MQ=255)
                                                                                        tgAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGttt                                       >  2:82181/1‑140 (MQ=255)
                                                                                        tgAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGttt                                       >  1:874982/1‑140 (MQ=255)
                                                                                           aTAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGt                                    >  1:449657/1‑140 (MQ=255)
                                                                                           aTAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGt                                    >  1:821230/1‑140 (MQ=255)
                                                                                               aacaacCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCg                                >  2:695501/1‑140 (MQ=255)
                                                                                               aacaacCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCg                                >  1:1072932/1‑140 (MQ=255)
                                                                                               aacaacCGGACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCg                                >  1:1068925/1‑140 (MQ=255)
                                                                                                       gACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTgcgc                        >  2:794322/1‑140 (MQ=255)
                                                                                                       gACCCCATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTgcgc                        <  2:1046497/140‑1 (MQ=255)
                                                                                                            cATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGt                   >  2:38083/1‑140 (MQ=255)
                                                                                                            cATCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGt                   >  2:1036512/1‑140 (MQ=255)
                                                                                                              tCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGTCa                 >  2:985849/1‑140 (MQ=255)
                                                                                                              tCGAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGTCa                 >  2:83240/1‑140 (MQ=255)
                                                                                                                gAGGAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCCGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGTCAcc               <  2:452684/140‑1 (MQ=255)
                                                                                                                  ggAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGTCACCgg             <  1:1172286/140‑1 (MQ=255)
                                                                                                                  ggAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGTCACCgg             >  2:1133137/1‑140 (MQ=255)
                                                                                                                  ggAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGTCACCgg             <  1:814480/140‑1 (MQ=255)
                                                                                                                  ggAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGTCACCgg             <  1:823601/140‑1 (MQ=255)
                                                                                                                   gAACGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGTCACCGGa            >  2:500498/1‑140 (MQ=255)
                                                                                                                    aaCGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGTCACCGGAg           <  2:1072932/140‑1 (MQ=255)
                                                                                                                    aaCGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGTCACCGGAg           >  1:3728/1‑140 (MQ=255)
                                                                                                                    aaCGGAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTCGGTGCCGGAGTGCGCGCAGTCACCGGAg           <  1:896050/140‑1 (MQ=255)
                                                                                                                       ggAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGTCACCGGAGCGt        >  2:565809/1‑140 (MQ=255)
                                                                                                                        gAGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGTCACCGGAGCGTa       <  1:1133137/140‑1 (MQ=255)
                                                                                                                         aGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGt                   >  1:686003/1‑127 (MQ=255)
                                                                                                                         aGTCCGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGt                   <  2:686003/127‑1 (MQ=255)
                                                                                                                            ccGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGTCACCGGAGCGTACACg   <  1:38083/140‑1 (MQ=255)
                                                                                                                             cGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGTCACCGGAGCGTACACGc  <  1:992262/140‑1 (MQ=255)
                                                                                                                             cGGCGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGTCACCGGAGCGTACACGc  <  2:350397/140‑1 (MQ=255)
                                                                                                                                |                                                                                                                                        
CGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTGCTGTAAAAGGCCGTCGGCGGTGCAGCCAGTTTGGTGCCGGAGTGCGCGCAGTCACCGGAGCGTACACGC  >  NC_000913/2746112‑2746376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A