Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 260 73.5 2514320 99.3% 2496719 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,798,530 (A)7→6 intergenic (‑35/‑634) stpA ← / → alaE DNA‑binding transcriptional repressor StpA with RNA chaperone activity/L‑alanine exporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,798,5240A.100.0% 356.9 / NA 82intergenic (‑29/‑640)stpA/alaEDNA‑binding transcriptional repressor StpA with RNA chaperone activity/L‑alanine exporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (35/47);  total (35/47)

GTGACAACCCTGAATTTTTCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCAAGATT  >  NC_000913/2798386‑2798653
                                                                                                                                          |                                                                                                                                    
gTGACAACCCTGAATTTTTCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCcaaaacaa                                                                                                                                     >  1:689224/1‑140 (MQ=255)
 tGACAACCCTGAATTTTTCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCcaaaacaaa                                                                                                                                    >  2:428855/1‑140 (MQ=255)
   aCAACCCTGAATTTTTCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAACaaaaa                                                                                                                                  >  1:136506/1‑140 (MQ=255)
         cTGAATTTTTCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTAtt                                                                                                                           <  2:586359/140‑1 (MQ=255)
            aaTTTTTCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTtcg                                                                                                                        >  1:698150/1‑140 (MQ=255)
            aaTTTTTCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTtcg                                                                                                                        <  1:917031/140‑1 (MQ=255)
               ttttCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTtcgtcg                                                                                                                     <  1:1154092/140‑1 (MQ=255)
                 ttCGAGCATTTCTTCAAGAGCGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTcgcg                                                                                                                   <  1:395765/140‑1 (MQ=255)
                 ttCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTtcgtcg                                                                                                                     <  1:540172/138‑1 (MQ=255)
                 ttCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTtcgtcg                                                                                                                     >  2:540172/1‑138 (MQ=255)
                 ttCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTcgcg                                                                                                                   <  2:1011308/140‑1 (MQ=255)
                 ttCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTcgcg                                                                                                                   <  2:755691/140‑1 (MQ=255)
                 ttCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTcgcg                                                                                                                   <  2:801456/140‑1 (MQ=255)
                 ttCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTcgcg                                                                                                                   <  2:986482/140‑1 (MQ=255)
                 ttCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTcgcg                                                                                                                   <  1:568524/140‑1 (MQ=255)
                 ttCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTcgcg                                                                                                                   <  2:602530/140‑1 (MQ=255)
                 ttCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTcgcg                                                                                                                   <  1:824945/140‑1 (MQ=255)
                 ttCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTcgcg                                                                                                                   <  2:1097795/140‑1 (MQ=255)
                 ttCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTcgcg                                                                                                                   <  2:136506/140‑1 (MQ=255)
                  tCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGt                                                                                                                  <  1:57809/140‑1 (MQ=255)
                          ttcttcAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAg                                                                                                          >  2:93764/1‑140 (MQ=255)
                              tcAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGAt                                                                                                      <  2:747632/140‑1 (MQ=255)
                              tcAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGAt                                                                                                      <  1:640268/140‑1 (MQ=255)
                              tcAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGAt                                                                                                      <  2:692288/140‑1 (MQ=255)
                              tcAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGAt                                                                                                      <  1:211048/140‑1 (MQ=255)
                               cAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGNNNNTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATc                                                                                                     <  1:373397/140‑1 (MQ=255)
                               cAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATc                                                                                                     <  1:178898/140‑1 (MQ=255)
                                aaGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTaa                                                                                                           <  1:458036/133‑1 (MQ=255)
                                aaGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTaa                                                                                                           >  2:458036/1‑133 (MQ=255)
                                   aaCGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTcgcg                                                                                                                   >  1:773388/1‑122 (MQ=255)
                                   aaCGTCAATGGAAAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTcgcg                                                                                                                   <  2:773388/122‑1 (MQ=255)
                                     cGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACttat                                                                                               >  1:804359/1‑140 (MQ=255)
                                     cGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACttat                                                                                               >  2:589314/1‑140 (MQ=255)
                                       tCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAg                                                                                                          <  2:1164669/127‑1 (MQ=255)
                                       tCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAg                                                                                                          >  1:1164669/1‑127 (MQ=255)
                                       tCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACttatta                                                                                             >  1:619441/1‑140 (MQ=255)
                                       tCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACttatta                                                                                             >  1:546637/1‑140 (MQ=255)
                                       tCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACttatta                                                                                             >  2:420564/1‑140 (MQ=255)
                                        cAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTATGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCg                                                                                                        >  1:110546/1‑128 (MQ=255)
                                        cAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTATGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCg                                                                                                        <  2:110546/128‑1 (MQ=255)
                                                aaTTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCcac                                                                                    <  1:243193/140‑1 (MQ=255)
                                                  ttCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACaa                                                                                  >  1:985775/1‑140 (MQ=255)
                                                          ccATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAt                                                                          <  2:698150/140‑1 (MQ=255)
                                                            aTCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑Ct                                                                     <  1:420564/140‑1 (MQ=255)
                                                            aTCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑Ct                                                                     <  1:428855/140‑1 (MQ=255)
                                                            aTCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑Ct                                                                     <  1:881330/140‑1 (MQ=255)
                                                            aTCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑Ct                                                                     <  2:350519/140‑1 (MQ=255)
                                                                 aCGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTa                                                                <  2:546637/140‑1 (MQ=255)
                                                                 aCGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTa                                                                <  1:93764/140‑1 (MQ=255)
                                                                     aGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGt                                                            >  1:166638/1‑140 (MQ=255)
                                                                       ggTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAt                                                                          >  2:243530/1‑127 (MQ=255)
                                                                       ggTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAt                                                                          <  1:243530/127‑1 (MQ=255)
                                                                           cGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTgcg                                                      >  2:87941/1‑140 (MQ=255)
                                                                               tGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCgag                                                  <  2:166638/140‑1 (MQ=255)
                                                                               tGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCgag                                                  >  1:971791/1‑140 (MQ=255)
                                                                                 ttATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGat                                                >  1:1000465/1‑140 (MQ=255)
                                                                                   aTTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGatta                                              >  2:978468/1‑140 (MQ=255)
                                                                                             tGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGt                                    >  2:303508/1‑140 (MQ=255)
                                                                                             tGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGt                                    >  2:505933/1‑140 (MQ=255)
                                                                                              gTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGtt                                   >  2:1236794/1‑140 (MQ=255)
                                                                                                 aCATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTAtt                                <  2:985775/140‑1 (MQ=255)
                                                                                                     tACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCt                            >  1:1055633/1‑140 (MQ=255)
                                                                                                     tACGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCt                            >  1:209781/1‑140 (MQ=255)
                                                                                                       cGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGa                          >  2:225188/1‑140 (MQ=255)
                                                                                                       cGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGa                          >  2:539336/1‑140 (MQ=255)
                                                                                                       cGGACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGa                          >  2:581053/1‑140 (MQ=255)
                                                                                                        ggACATAATAAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGaa                         <  1:428178/140‑1 (MQ=255)
                                                                                                            ataataAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAActc                     >  1:979308/1‑140 (MQ=255)
                                                                                                            ataataAAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAActc                     <  1:225188/140‑1 (MQ=255)
                                                                                                              aataaAGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACtctc                   >  2:206213/1‑140 (MQ=255)
                                                                                                                   aGAAAACCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGaat              >  2:875174/1‑140 (MQ=255)
                                                                                                                       aaCCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTNGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAAtt          >  1:194681/1‑140 (MQ=255)
                                                                                                                        aCCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCAGCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGaataa            >  2:1244912/1‑140 (MQ=255)
                                                                                                                        aCCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGaat              >  2:743963/1‑135 (MQ=255)
                                                                                                                        aCCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGaat              <  1:743963/135‑1 (MQ=255)
                                                                                                                        aCCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAAttt         <  2:1055633/140‑1 (MQ=255)
                                                                                                                        aCCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAAttt         >  2:350678/1‑140 (MQ=255)
                                                                                                                        aCCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAAttt         >  1:232678/1‑140 (MQ=255)
                                                                                                                        aCCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAAttt         <  2:194681/140‑1 (MQ=255)
                                                                                                                        aCCTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAAttt         <  1:1236794/140‑1 (MQ=255)
                                                                                                                          cTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCa       <  2:619441/140‑1 (MQ=255)
                                                                                                                          cTTTTAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCa       <  1:87941/140‑1 (MQ=255)
                                                                                                                             ttAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCAAGa    <  1:978468/140‑1 (MQ=255)
                                                                                                                              tAACGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCAAGAt   <  2:10403/140‑1 (MQ=255)
                                                                                                                               aaCGCCAAAAC‑AAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCAAGAtt  <  2:1000465/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                    
GTGACAACCCTGAATTTTTCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAAT‑‑‑CTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCAAGATT  >  NC_000913/2798386‑2798653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A