Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,804,419	G→A	M306I (ATG→ATA)	nrdF →	ribonucleoside‑diphosphate reductase 2 subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,804,419	0	G	A	100.0%	187.5 / NA	53	M306I (ATG→ATA)	nrdF	ribonucleoside‑diphosphate reductase 2 subunit beta
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/29); total (24/29)

TTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACAC  >  NC_000913/2804285‑2804555

ttGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGaa                                                                                                                                     >  1:865229/1‑140 (MQ=255)

 tgatgaATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGaaa                                                                                                                                    >  2:301492/1‑140 (MQ=255)

 tgatgaATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGaaa                                                                                                                                    <  1:870462/140‑1 (MQ=255)

 tgatgaATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGaaa                                                                                                                                    >  2:728247/1‑140 (MQ=255)

 tgatgaATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGaaa                                                                                                                                    >  1:370707/1‑140 (MQ=255)

 tgatgaATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGACTCCTCTTATGTGATAGGGaaa                                                                                                                                    >  1:886656/1‑140 (MQ=255)

   atgaATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGc                                                                                                                                  <  2:777723/140‑1 (MQ=255)

     gaATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCgg                                                                                                                                <  2:370707/140‑1 (MQ=255)

      aaTCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGt                                                                                                                               <  1:728247/140‑1 (MQ=255)

       aTCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGtt                                                                                                                              >  1:247878/1‑140 (MQ=255)

         cTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGa                                                                                                                            <  1:1186721/140‑1 (MQ=255)

           gggCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGaaa                                                                                                                          <  1:1059519/140‑1 (MQ=255)

            ggCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAAc                                                                                                                         <  2:315604/140‑1 (MQ=255)

                 cGAACCGTTATTTCCCGCAGAAATGGCGGAAGTTAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGt                                                                                                                               <  2:126012/129‑1 (MQ=255)

                 cGAACCGTTATTTCCCGCAGAAATGGCGGAAGTTAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGt                                                                                                                               >  1:126012/1‑129 (MQ=255)

                  gAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGc                                                                                                                                  <  1:451230/125‑1 (MQ=255)

                  gAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGc                                                                                                                                  >  2:451230/1‑125 (MQ=255)

                      cGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGaa                                                                                                               >  2:1159446/1‑140 (MQ=255)

                         tATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGAc                                                                                                            <  1:976550/140‑1 (MQ=255)

                           tttCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTg                                                                                                          >  1:347275/1‑140 (MQ=255)

                                  cAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTc                                                                                                   >  1:161898/1‑140 (MQ=255)

                                  cAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTc                                                                                                   <  2:161898/140‑1 (MQ=255)

                                     aaaTGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGa                                                                                                >  1:689924/1‑140 (MQ=255)

                                           cGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGgtgt                                                                                          >  1:1175756/1‑140 (MQ=255)

                                             gAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTa                                                                                        <  2:554668/140‑1 (MQ=255)

                                             gAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTa                                                                                        >  1:252061/1‑140 (MQ=255)

                                                  gAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTc                                                                                   <  1:1159446/140‑1 (MQ=255)

                                                          cAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAatat                                                                           >  2:631246/1‑140 (MQ=255)

                                                          cAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAatat                                                                           >  1:197914/1‑140 (MQ=255)

                                                            aTCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCa                                                                         <  1:537480/140‑1 (MQ=255)

                                                            aTCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCa                                                                         <  2:1175756/140‑1 (MQ=255)

                                                               cTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTa                                                                      <  1:301492/140‑1 (MQ=255)

                                                                   ccgcGCTTTCGGCGAATGGCGATGAAAATCAGGATTTCTTTTCCGGTTCAGGCTACGCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCg                                                                  <  2:197913/140‑1 (MQ=255)

                                                                   ccgcGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCg                                                                  >  1:133130/1‑140 (MQ=255)

                                                                   ccgcGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCg                                                                  <  2:197914/140‑1 (MQ=255)

                                                                       gcTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCgcagc                                                              >  1:792724/1‑140 (MQ=255)

                                                                        cTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCgcagca                                                             <  1:906565/140‑1 (MQ=255)

                                                                         tttCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGcagcag                                                            <  2:252061/140‑1 (MQ=255)

                                                                         tttCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGcagcag                                                            <  2:133130/140‑1 (MQ=255)

                                                                         tttCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGcagcag                                                            <  2:792724/140‑1 (MQ=255)

                                                                                   tGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAAtt                                                  >  1:847215/1‑140 (MQ=255)

                                                                                                     tttCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCaaa                                         >  2:437755/1‑131 (MQ=255)

                                                                                                     tttCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCaaa                                         <  1:437755/131‑1 (MQ=255)

                                                                                                     tttCTTTTCCGGTTCAGGCTCCTCTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCttttt                                >  1:667800/1‑140 (MQ=255)

                                                                                                                        tcctcTTATGTGATAGGGAAAGCGGTTGAAACAGACGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTTCCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTtgtg             <  2:865229/140‑1 (MQ=255)

                                                                                                                        tcctcTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTGTCACGCAAATAATTtgtg             <  2:689924/140‑1 (MQ=255)

                                                                                                                        tcctcTTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTtgtg             <  2:667800/140‑1 (MQ=255)

                                                                                                                           tctTATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtggtg          >  2:22588/1‑140 (MQ=255)

                                                                                                                              tATGTGATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATc       <  1:775959/140‑1 (MQ=255)

                                                                                                                                tgtgATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTa     >  2:1130140/1‑140 (MQ=255)

                                                                                                                                tgtgATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTa     <  1:732817/140‑1 (MQ=255)

                                                                                                                                tgtgATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTa     >  2:732817/1‑140 (MQ=255)

                                                                                                                                   gATAGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTacac  <  2:200436/140‑1 (MQ=255)

TTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACAC  >  NC_000913/2804285‑2804555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A