Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,890,797	G→A	P368S (CCG→TCG)	cysJ ←	sulfite reductase, flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,890,797	0	G	A	100.0%	203.1 / NA	58	P368S (CCG→TCG)	cysJ	sulfite reductase, flavoprotein subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (31/27); total (31/27)

TTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGGGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTG  >  NC_000913/2890660‑2890927

ttCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCgagg                                                                                                                                  <  2:1127660/140‑1 (MQ=255)

        cgtAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGc                                                                                                                          <  1:1135209/140‑1 (MQ=255)

        cgtAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGc                                                                                                                          >  2:191192/1‑140 (MQ=255)

           aaCGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCAcc                                                                                                                       <  1:191192/140‑1 (MQ=255)

            aCGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCa                                                                                                                      <  1:665705/140‑1 (MQ=255)

            aCGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCa                                                                                                                      <  1:438544/140‑1 (MQ=255)

                accacaccaACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTc                                                                                                                  <  2:580716/140‑1 (MQ=255)

                 ccacaccaACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTca                                                                                                                 <  1:1081535/140‑1 (MQ=255)

                  cacaccaACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTcaa                                                                                                                >  2:617834/1‑140 (MQ=255)

                  cacaccaACGGTGACGTGTACTTCGTTCTCGACTTCCGCATGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTcaa                                                                                                                >  2:296526/1‑140 (MQ=255)

                    caccaACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCAc                                                                                                                        <  2:938930/130‑1 (MQ=255)

                    caccaACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCAc                                                                                                                        >  1:938930/1‑130 (MQ=255)

                    caccaACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTcaaca                                                                                                              >  2:704580/1‑140 (MQ=255)

                            gTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGGATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGcgt                                                                                                      >  2:1131343/1‑140 (MQ=255)

                            gTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGGATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGcgt                                                                                                      <  1:1131343/140‑1 (MQ=255)

                               aCGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGcgtcgt                                                                                                   >  2:81259/1‑140 (MQ=255)

                               aCGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGcgtcgt                                                                                                   >  2:1074439/1‑140 (MQ=255)

                                 gtgtACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATAAAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGGCAACAATCGGCGtcgtcg                                                                                                 <  2:483502/140‑1 (MQ=255)

                                     aCTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCgcggc                                                                                             >  1:216781/1‑140 (MQ=255)

                                        tCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTa                                                                                          <  2:1166307/140‑1 (MQ=255)

                                         cGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGcgt                                                                                                      <  2:710892/127‑1 (MQ=255)

                                         cGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGcgt                                                                                                      >  1:710892/1‑127 (MQ=255)

                                                        ccTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCt                                                                          <  1:643645/140‑1 (MQ=255)

                                                                           aTACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCccacc                                                              >  2:371866/1‑133 (MQ=255)

                                                                           aTACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCccacc                                                              <  1:371866/133‑1 (MQ=255)

                                                                            tACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGgcag                                                      <  1:617834/140‑1 (MQ=255)

                                                                            tACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGgcag                                                      <  1:296526/140‑1 (MQ=255)

                                                                               aGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGcagcag                                                   >  1:659155/1‑140 (MQ=255)

                                                                                gACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAgt                                                  >  1:823823/1‑140 (MQ=255)

                                                                                 aCGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAgtg                                                 >  2:1004293/1‑140 (MQ=255)

                                                                                    cggcgTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGTGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGttt                                              >  2:896625/1‑140 (MQ=255)

                                                                                    cggcgTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGttt                                              >  1:46038/1‑140 (MQ=255)

                                                                                    cggcgTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGttt                                              <  2:627716/140‑1 (MQ=255)

                                                                                        gTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGTGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACt                                          <  1:896625/140‑1 (MQ=255)

                                                                                        gTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACt                                          <  1:1004293/140‑1 (MQ=255)

                                                                                        gTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACt                                          <  2:846462/140‑1 (MQ=255)

                                                                                         tCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTg                                         >  2:77262/1‑140 (MQ=255)

                                                                                              gggCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGt                                    <  1:870694/140‑1 (MQ=255)

                                                                                                 cgcAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAg                                 >  2:602683/1‑140 (MQ=255)

                                                                                                   cagcagATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCg                               >  2:1182230/1‑140 (MQ=255)

                                                                                                      cagATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTgg                            >  2:782372/1‑140 (MQ=255)

                                                                                                        gATTAATTAGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCg                          <  1:602683/140‑1 (MQ=255)

                                                                                                          ttaattaGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTa                        <  2:659155/140‑1 (MQ=255)

                                                                                                              ttaGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTAATtc                    >  1:929362/1‑140 (MQ=255)

                                                                                                              ttaGCGCTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTAATtc                    >  1:895512/1‑140 (MQ=255)

                                                                                                                  cgcTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTAATTCTcaa                >  2:159784/1‑140 (MQ=255)

                                                                                                                  cgcTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTAATTCTcaa                >  2:1137661/1‑140 (MQ=255)

                                                                                                                  cgcTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTAATTCTcaa                >  1:872791/1‑140 (MQ=255)

                                                                                                                   gcTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTAATTCTcaac               >  1:690765/1‑140 (MQ=255)

                                                                                                                   gcTTCGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTAATTCTcaac               >  1:64193/1‑140 (MQ=255)

                                                                                                                       cGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATg           >  1:535364/1‑140 (MQ=255)

                                                                                                                       cGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATg           >  1:485899/1‑140 (MQ=255)

                                                                                                                       cGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATg           >  2:988299/1‑140 (MQ=255)

                                                                                                                       cGGCATCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCAACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATg           >  1:424545/1‑140 (MQ=255)

                                                                                                                            tCAAGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGc      <  2:64193/140‑1 (MQ=255)

                                                                                                                              aaGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCgg    <  1:337784/140‑1 (MQ=255)

                                                                                                                              aaGCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCgg    <  1:159784/140‑1 (MQ=255)

                                                                                                                                gCTGTGCCGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGgtg  <  1:782372/140‑1 (MQ=255)

                                                                                                                                      ccGAGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTgg       <  2:404587/129‑1 (MQ=255)

TTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGGGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGCTGTAACTTCGCTTTATCGCCCACCAGCGGCAGCAGTGTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTG  >  NC_000913/2890660‑2890927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A