Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 260 73.5 2514320 99.3% 2496719 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,920,941 C→T G387D (GGC→GAC)  gudP ← galactarate/glucarate/glycerate transporter GudP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,920,9410CT100.0% 290.4 / NA 84G387D (GGC→GAC) gudPgalactarate/glucarate/glycerate transporter GudP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/45);  total (39/45)

GGTAGCTCAGTACCGCGATTAAGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGCCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAA  >  NC_000913/2920804‑2921077
                                                                                                                                         |                                                                                                                                        
ggTAGCTCAGTACCGCGATTAAGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCa                                                                                                                                        >  2:507326/1‑140 (MQ=255)
     cTCAGTACCGCGATTAAGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGa                                                                                                                                   >  1:1017521/1‑140 (MQ=255)
     cTCAGTACCGCGATTAAGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGa                                                                                                                                   >  2:657895/1‑140 (MQ=255)
     cTCAGTACCGCGATTAAGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGa                                                                                                                                   >  2:553170/1‑140 (MQ=255)
       cAGTACCGCGATTAAGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGaaa                                                                                                                                 <  1:1172443/140‑1 (MQ=255)
          tACCGCGATTAAGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGAc                                                                                                                              >  1:569583/1‑140 (MQ=255)
          tACCGCGATTAAGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGAc                                                                                                                              >  1:452738/1‑140 (MQ=255)
           aCCGCGATTAAGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGAcc                                                                                                                             >  1:611932/1‑140 (MQ=255)
            ccGCGATTAAGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCg                                                                                                                            <  2:611932/140‑1 (MQ=255)
               cgATTAAGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTg                                                                                                                         >  2:967016/1‑140 (MQ=255)
               cgATTAAGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTg                                                                                                                         >  2:1109026/1‑140 (MQ=255)
               cgATTAAGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTg                                                                                                                         >  1:825146/1‑140 (MQ=255)
                gATTAAGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGa                                                                                                                        <  2:659835/140‑1 (MQ=255)
                gATTAAGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGa                                                                                                                        <  2:1153498/140‑1 (MQ=255)
                    aaGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCg                                                                                                                            >  1:157089/1‑132 (MQ=255)
                    aaGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCg                                                                                                                            <  2:157089/132‑1 (MQ=255)
                    aaGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGAtctc                                                                                                                    >  2:740646/1‑140 (MQ=255)
                    aaGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGAtctc                                                                                                                    >  2:357911/1‑140 (MQ=255)
                          tGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTgg                                                                                                              <  2:787844/140‑1 (MQ=255)
                            aaCACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCttt                                                                                                                 <  1:1019769/135‑1 (MQ=255)
                            aaCACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCttt                                                                                                                 >  2:1019769/1‑135 (MQ=255)
                            aaCACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGgcg                                                                                                            >  1:259617/1‑140 (MQ=255)
                            aaCACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGgcg                                                                                                            <  2:687534/140‑1 (MQ=255)
                             acacCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGgcgc                                                                                                           <  2:411974/140‑1 (MQ=255)
                              cacCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGcgcg                                                                                                          <  1:913197/140‑1 (MQ=255)
                                 cAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTa                                                                                                       <  2:197157/140‑1 (MQ=255)
                                 cAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTa                                                                                                       <  2:1184425/140‑1 (MQ=255)
                                 cAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTa                                                                                                       >  1:197157/1‑140 (MQ=255)
                                     aTAAATCAGCGCCCCATTAAACTAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTg                                                                                                   >  2:83550/1‑140 (MQ=255)
                                     aTAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTg                                                                                                   <  1:979784/140‑1 (MQ=255)
                                      tAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGc                                                                                                  <  1:194908/140‑1 (MQ=255)
                                           cAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTa                                                                                             <  1:55224/140‑1 (MQ=255)
                                           cAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTa                                                                                             <  1:967016/140‑1 (MQ=255)
                                              cgcCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTg                                                                                          >  2:71191/1‑140 (MQ=255)
                                              cgcCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTg                                                                                          >  2:394210/1‑140 (MQ=255)
                                              cgcCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTg                                                                                          >  1:1053144/1‑140 (MQ=255)
                                              cgcCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTg                                                                                          >  1:509568/1‑140 (MQ=255)
                                              cgcCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTg                                                                                          >  1:318824/1‑140 (MQ=255)
                                               gcCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGc                                                                                         >  2:768953/1‑140 (MQ=255)
                                               gcCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGc                                                                                         >  1:14539/1‑140 (MQ=255)
                                                ccccATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGcc                                                                                        <  1:540321/140‑1 (MQ=255)
                                                  ccATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCt                                                                                                    <  1:748215/126‑1 (MQ=255)
                                                  ccATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCt                                                                                                    >  2:748215/1‑126 (MQ=255)
                                                   cATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCaa                                                                                     >  2:717521/1‑140 (MQ=255)
                                                     ttAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAAcc                                                                                   <  1:72272/140‑1 (MQ=255)
                                                     ttAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAAcc                                                                                   <  1:657895/140‑1 (MQ=255)
                                                             gCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTa                                                                                             >  1:1066232/1‑122 (MQ=255)
                                                             gCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTa                                                                                             <  2:1066232/122‑1 (MQ=255)
                                                               cAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCg                                                                         <  1:740646/140‑1 (MQ=255)
                                                               cAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCg                                                                         <  2:242251/140‑1 (MQ=255)
                                                                    gTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGcc                                                                    <  2:343097/140‑1 (MQ=255)
                                                                    gTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGcc                                                                    <  2:825146/140‑1 (MQ=255)
                                                                    gTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGcc                                                                    <  1:717521/140‑1 (MQ=255)
                                                                    gTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGcc                                                                    <  1:507326/140‑1 (MQ=255)
                                                                         aaCGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTAc                                                               <  2:14539/140‑1 (MQ=255)
                                                                          aCGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTAcc                                                              >  1:563290/1‑140 (MQ=255)
                                                                              tataACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCgaag                                                          >  2:653527/1‑140 (MQ=255)
                                                                                      aTTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAg                                                  >  2:424168/1‑140 (MQ=255)
                                                                                      aTTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAg                                                  <  2:604808/140‑1 (MQ=255)
                                                                                        tGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAgcg                                                >  2:1009259/1‑140 (MQ=255)
                                                                                          cGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCACTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGcc                                              >  1:831080/1‑140 (MQ=255)
                                                                                             ttGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATa                                           <  2:16166/140‑1 (MQ=255)
                                                                                             ttGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATa                                           <  1:653527/140‑1 (MQ=255)
                                                                                                      cGATGCCAGAAATGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCgatg                                  <  1:764236/140‑1 (MQ=255)
                                                                                                               aaaTGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCAc                         >  2:936033/1‑140 (MQ=255)
                                                                                                               aaaTGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCAc                         >  2:1056753/1‑140 (MQ=255)
                                                                                                                aaTGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACt                        <  2:259617/140‑1 (MQ=255)
                                                                                                                 aTGTTACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTc                       <  1:1009259/140‑1 (MQ=255)
                                                                                                                    ttACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAAc                    >  2:403592/1‑140 (MQ=255)
                                                                                                                     tACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACg                   <  2:569583/140‑1 (MQ=255)
                                                                                                                     tACCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACg                   <  1:389643/140‑1 (MQ=255)
                                                                                                                      aCCGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGt                  <  1:404595/140‑1 (MQ=255)
                                                                                                                       ccGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGtt                 <  1:71191/140‑1 (MQ=255)
                                                                                                                       ccGAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGtt                 <  1:394210/140‑1 (MQ=255)
                                                                                                                         gAACATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGa               <  1:819973/140‑1 (MQ=255)
                                                                                                                          aaCATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGAc              >  1:907136/1‑140 (MQ=255)
                                                                                                                           aCATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACg             <  2:318824/140‑1 (MQ=255)
                                                                                                                            cATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGt            >  2:1008855/1‑140 (MQ=255)
                                                                                                                            cATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGt            >  2:717899/1‑140 (MQ=255)
                                                                                                                            cATGTTGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGt            <  2:902611/140‑1 (MQ=255)
                                                                                                                                 tGAACAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTg       >  1:949590/1‑140 (MQ=255)
                                                                                                                                     cAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGa   <  1:1056753/140‑1 (MQ=255)
                                                                                                                                     cAGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGa   <  1:936033/140‑1 (MQ=255)
                                                                                                                                      aGGTCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGaa  <  1:717899/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
GGTAGCTCAGTACCGCGATTAAGGCATGAACACCAACATAAATCAGCGCCCCATTAAACGAGCCAGTCGTGCCAACGATATAACCAATTGCGATTGGCGTGACGATGCCAGAAATGTTACCGAACATGTTGAACAGGCCACCGGAAAGACCGCTGATCTCTTTTGGCGCGGTATCTGCCATTACTGCCCAACCCAGCGCCCCGATGCCCTTACCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAA  >  NC_000913/2920804‑2921077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A