Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,973,022	T→C	T281A (ACC→GCC)	lplT ←	lysophospholipid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,973,022	0	T	C	100.0%	207.3 / NA	59	T281A (ACC→GCC)	lplT	lysophospholipid transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/31); total (28/31)

GGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGTTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGA  >  NC_000913/2972889‑2973153

ggAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAg                                                                                                                               <  1:299478/140‑1 (MQ=255)

     gACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGtaa                                                                                                                          <  2:524677/140‑1 (MQ=255)

       cAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGtaact                                                                                                                        >  2:946857/1‑140 (MQ=255)

             aGCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTc                                                                                                                  <  1:946857/140‑1 (MQ=255)

               ccccccATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGc                                                                                                                >  1:596079/1‑140 (MQ=255)

                                      caacaaGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGa                                                                                         >  2:208959/1‑140 (MQ=255)

                                           aGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCa                                                                                    <  2:1014733/140‑1 (MQ=255)

                                           aGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCa                                                                                    >  1:894343/1‑140 (MQ=255)

                                              cATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATc                                                                                 >  1:83344/1‑140 (MQ=255)

                                                   gCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTAc                                                                            >  2:424511/1‑140 (MQ=255)

                                                     tGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCa                                                                          <  1:1150070/140‑1 (MQ=255)

                                                       gcagcaGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATc                                                                        >  2:84251/1‑140 (MQ=255)

                                                       gcagcaGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATc                                                                        >  2:44251/1‑140 (MQ=255)

                                                        cagcagCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGa                                                                                         <  2:1053641/122‑1 (MQ=255)

                                                        cagcagCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGa                                                                                         >  1:1053641/1‑122 (MQ=255)

                                                         agcagcTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATcgc                                                                      <  2:360492/140‑1 (MQ=255)

                                                         agcagcTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATcgc                                                                      <  1:44251/140‑1 (MQ=255)

                                                          gcagcTCGTGTTGCGGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATcgcg                                                                     >  2:588809/1‑140 (MQ=255)

                                                             gcTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTg                                                                  >  1:1232803/1‑140 (MQ=255)

                                                             gcTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGAACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTg                                                                  <  1:847567/140‑1 (MQ=255)

                                                              cTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTga                                                                 >  2:132959/1‑140 (MQ=255)

                                                                cGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTgaga                                                               <  2:60999/140‑1 (MQ=255)

                                                                    ttGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAgg                                                           <  2:632919/140‑1 (MQ=255)

                                                                    ttGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAgg                                                           <  2:382993/140‑1 (MQ=255)

                                                                     tGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGt                                                          >  2:1068668/1‑140 (MQ=255)

                                                                              aaaaTCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGc                                                 <  2:1232803/140‑1 (MQ=255)

                                                                                  tCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTa                                             <  1:588809/140‑1 (MQ=255)

                                                                                   cAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGtt                                              <  2:687752/138‑1 (MQ=255)

                                                                                   cAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGtt                                              >  1:687752/1‑138 (MQ=255)

                                                                                   cAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGAAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTAt                                            <  1:1196129/140‑1 (MQ=255)

                                                                                      tACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCgg                                         >  1:718988/1‑140 (MQ=255)

                                                                                         caccacGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTaa                                      >  2:134379/1‑140 (MQ=255)

                                                                                              cGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGccc                                 >  1:1007407/1‑140 (MQ=255)

                                                                                                ccAATCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAg                               <  1:10614/140‑1 (MQ=255)

                                                                                                   aTCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAgcgc                            >  1:853377/1‑140 (MQ=255)

                                                                                                   aTCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAgcgc                            >  2:581200/1‑140 (MQ=255)

                                                                                                   aTCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAgcgc                            <  1:482861/140‑1 (MQ=255)

                                                                                                   aTCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAgcgc                            <  1:208959/140‑1 (MQ=255)

                                                                                                   aTCAAAATCCCGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCATAGCGTTATCGGTAATGCCCAgcgc                            >  2:639879/1‑140 (MQ=255)

                                                                                                           cccGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTa                    <  2:431067/140‑1 (MQ=255)

                                                                                                             cGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCa                          <  1:399993/132‑1 (MQ=255)

                                                                                                             cGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCa                          >  2:399993/1‑132 (MQ=255)

                                                                                                             cGGCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTAcc                  >  1:625869/1‑140 (MQ=255)

                                                                                                              ggCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTAccc                 >  1:547901/1‑140 (MQ=255)

                                                                                                               gCTGGCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCca                <  2:207855/140‑1 (MQ=255)

                                                                                                                  ggCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAg             <  2:204105/140‑1 (MQ=255)

                                                                                                                  ggCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAg             <  1:424511/140‑1 (MQ=255)

                                                                                                                  ggCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAg             <  2:894343/140‑1 (MQ=255)

                                                                                                                   gCATACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGc            <  1:84251/140‑1 (MQ=255)

                                                                                                                     aTACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCAc          <  2:644249/140‑1 (MQ=255)

                                                                                                                     aTACAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCAc          <  2:1005451/140‑1 (MQ=255)

                                                                                                                       aCAGCGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCa        <  2:853377/140‑1 (MQ=255)

                                                                                                                           cGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAg    >  1:623243/1‑140 (MQ=255)

                                                                                                                           cGTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAg    >  2:698211/1‑140 (MQ=255)

                                                                                                                            gTGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAgg   <  2:596079/140‑1 (MQ=255)

                                                                                                                             tGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTAcc                  >  2:508698/1‑124 (MQ=255)

                                                                                                                             tGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTAcc                  <  1:508698/124‑1 (MQ=255)

                                                                                                                             tGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGa  >  2:487967/1‑140 (MQ=255)

                                                                                                                             tGACACGGCTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGa  >  1:23074/1‑140 (MQ=255)

GGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGTTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGA  >  NC_000913/2972889‑2973153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A