Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R1
|
260 |
73.5 |
2514320 |
99.3% |
2496719 |
138.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,160,896 |
T→C |
D84G (GAC→GGC) |
ygiQ ← |
radical SAM superfamily protein YgiQ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,160,896 | 0 | T | C | 96.1%
| 165.0
/ ‑2.1
| 51 | D84G (GAC→GGC) | ygiQ | radical SAM superfamily protein YgiQ |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (21/28); total (22/29) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 |
GGCGTGTAGGCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGGTGATCGACATACGCGTCGCCAGTAACCAAAATGATGTCGCAGCTATCCCAGCCAAGTTG > NC_000913/3160761‑3161032
|
ggCGTGTAGGCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCgg > 1:504800/1‑140 (MQ=255)
ggCGTGTAGGCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCAT‑‑AGTCGTCTTTGCTGCTCCAGCCCGGCt < 1:1171386/140‑1 (MQ=255)
cGTGTAGGCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCgg > 1:426241/1‑138 (MQ=255)
cGTGTAGGCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCgg < 2:426241/138‑1 (MQ=255)
tgtAGGCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTgg < 1:811185/140‑1 (MQ=255)
tAGGCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGc > 1:553071/1‑140 (MQ=255)
tCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCgatgat > 1:91254/1‑140 (MQ=255)
cGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCCAATCCATTTTGCCAGCAGGAACACCGAAAAACAAATTTGGTTTATCCCGACGCACAAAGTCGTCTTTGCTGCTCCAACCCGGCTGGGCGCTGATc > 2:1223016/1‑140 (MQ=255)
cATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCg > 1:473767/1‑140 (MQ=255)
gTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGa < 2:248542/140‑1 (MQ=255)
aCGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGc < 2:1110998/140‑1 (MQ=255)
ggCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGccc < 1:159308/140‑1 (MQ=255)
ggCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGccc < 2:553071/140‑1 (MQ=255)
gCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCt > 1:293931/1‑140 (MQ=255)
cGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATc > 1:823771/1‑123 (MQ=255)
cGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATc < 2:823771/123‑1 (MQ=255)
cGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTg < 2:932860/140‑1 (MQ=255)
gATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTgc < 1:1083266/140‑1 (MQ=255)
gATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTgc < 1:171869/140‑1 (MQ=255)
gATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAACAGTAACACCGAAAAACAGATTCGGTTTACCCAAACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTgc > 2:254646/1‑140 (MQ=255)
aTCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTgcg > 2:374534/1‑140 (MQ=255)
aTCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTgcg < 1:374534/140‑1 (MQ=255)
aTCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTgcg < 1:240484/140‑1 (MQ=255)
cggcggTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCt < 1:632409/140‑1 (MQ=255)
gcggTATAACGGTTGATCATTGAATCCATGTTGCCAGCAGTAACTCCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGCTCCCGACGCGAAAGCCCTGCGCTTc < 1:1223016/140‑1 (MQ=255)
taACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCAt > 2:844519/1‑140 (MQ=255)
gTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGAc < 1:769330/140‑1 (MQ=255)
tGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCg < 2:91254/140‑1 (MQ=255)
gATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGc > 1:1193916/1‑140 (MQ=255)
gATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGc > 1:415115/1‑140 (MQ=255)
gATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGc > 1:245153/1‑140 (MQ=255)
gATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGc > 1:795828/1‑140 (MQ=255)
atcatcGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCa < 1:844519/140‑1 (MQ=255)
aaTCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGcc < 2:660905/140‑1 (MQ=255)
aaTCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGcc < 2:415115/140‑1 (MQ=255)
cATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATcc < 1:715532/140‑1 (MQ=255)
tGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCg < 2:683076/140‑1 (MQ=255)
cagcagTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCtt < 2:206616/140‑1 (MQ=255)
cagTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTggg > 1:1210979/1‑140 (MQ=255)
tAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGGTGa > 1:810530/1‑140 (MQ=255)
aaaCAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGGTGATCGACATAcg > 2:732334/1‑140 (MQ=255)
aaaCAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGGTGATCGACATAcg > 1:1111828/1‑140 (MQ=255)
tCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGGTGATCGACATACGCGTCGCCa > 2:758135/1‑140 (MQ=255)
cGCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCACATCGCCATCCCGTAGCTTGGGCGATCGCCATCCGCGTCGCCAGTAAACAAAatgct > 2:654482/1‑138 (MQ=255)
gCATAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGGTGATCGACATACGCGTCGCCAGTAACC‑AAATGATGt < 1:36416/140‑1 (MQ=255)
tAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGGTGATCGACATACGCGTCGCCAGTAACCAAAATGATGTCg > 1:904925/1‑140 (MQ=255)
tAAAGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGGTGATCGACATACGCGTCGCCAGTAACCAAAATGATGTCg > 2:725531/1‑140 (MQ=255)
aGTCGTCTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGGTGATCGACATACGCGTCGCCAGTAACCAAAATGATGTCGCAg < 1:254148/140‑1 (MQ=255)
gtcTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGGTGATCGACATACGCGTCGCCAGTAACCAAAATGATGTCGCAGCTAt < 2:473767/140‑1 (MQ=255)
tcTTTGCTGCTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGGTGATCGACATACGCGTCGCCAGTAACCAAAATGATGTCGCAGCTATc > 1:1145944/1‑140 (MQ=255)
tgctgcTCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGGTGATCGACATACGCGTCGCCAGTAACCAAAATGATGTCGCAGCTATCCCAg < 2:795828/140‑1 (MQ=255)
gctgctCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGGTGATCGACATACGCGTCGCCAGTAACCAAAATGATGTCGCAGCTATCCCAGc > 2:651595/1‑140 (MQ=255)
gcggctCCAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGGTGATCGACATACGCGTCGCCAGTAAACAAAATGATGTCGCAGCTATCCCAGc < 1:654482/137‑1 (MQ=255)
gctCCAGTCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGGTGATCGACATACGCGTCGCCAGTAACCAAAATGATGTCGCAGCTATCCCAGCCaa < 1:758135/140‑1 (MQ=255)
cAGCCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGGTGATCGACATACGCGTCGCCAGTAACCAAAATGATGTCGCAGCTATCCCAGCCAAGTTg < 2:609032/140‑1 (MQ=255)
|
GGCGTGTAGGCATCGTCATGACGTAAACGGCGATCGGCGGTATAACGGTTGATCATCGAATCCATGTTGCCAGCAGTAACACCGAAAAACAGATTCGGTTTACCCAGACGCATAAAGTCGTCTTTGCTGCTCCAGTCCGGCTGGGCGATGATCCCGACGCGAAAGCCCTGCGCTTCCAGCATACGACCGCAAATCGCCATCCCGAAGCTTGGGTGATCGACATACGCGTCGCCAGTAACCAAAATGATGTCGCAGCTATCCCAGCCAAGTTG > NC_000913/3160761‑3161032
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
