Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,406,593	C→T	A226V (GCG→GTG)	accC →	biotin carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,406,593	0	C	T	100.0%	177.3 / NA	51	A226V (GCG→GTG)	accC	biotin carboxylase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/23); total (28/23)

CCATCTCCATGACCCGTGCGGAAGCGAAAGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGCGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGT  >  NC_000913/3406458‑3406729

ccATCTCCATGACCCGTGCGGAAGCGAAAGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGaa                                                                                                                                      <  2:961162/140‑1 (MQ=255)

   tctcCATGACCCGTGCGGAAGCGAAAGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGt                                                                                                                                   >  1:1177333/1‑140 (MQ=255)

    ctcCATGACCCGTGCGGAAGCGAAAGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTg                                                                                                                                  >  1:875379/1‑140 (MQ=255)

    ctcCATGACCCGTGCGGAAGCGAAAGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTg                                                                                                                                  >  2:583777/1‑140 (MQ=255)

       cATGACCCGTGCGGAAGCGAAAGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACt                                                                                                                               <  2:1177333/140‑1 (MQ=255)

        aTGACCCGTGCGGAAGCGAAAGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTg                                                                                                                              <  2:824584/140‑1 (MQ=255)

        aTGACCCGTGCGGAAGCGAAAGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTg                                                                                                                              <  2:1245755/140‑1 (MQ=255)

         tGACCCGTGCGGAAGCGAAAGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGc                                                                                                                             >  1:167254/1‑140 (MQ=255)

         tGACCCGTGCGGAAGCGAAAGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGc                                                                                                                             >  1:666801/1‑140 (MQ=255)

            cccGTGCGGAAGCGAAAGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTcc                                                                                                                          <  2:167254/140‑1 (MQ=255)

             ccGTGCGGAAGCGAAAGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCa                                                                                                                         <  1:583777/140‑1 (MQ=255)

               gTGCGGAAGCGAAAGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATg                                                                                                                       >  2:589947/1‑140 (MQ=255)

                tGCGGAAGCGAAAGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGc                                                                                                                      >  2:712219/1‑140 (MQ=255)

                        cGAAAGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAAcgccgc                                                                                                              <  2:1083295/140‑1 (MQ=255)

                          aaaGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAGAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAAcgccg                                                                                                               >  1:429414/1‑137 (MQ=255)

                          aaaGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAGAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAAcgccg                                                                                                               <  2:429414/137‑1 (MQ=255)

                          aaaGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGcca                                                                                                            >  2:1242574/1‑140 (MQ=255)

                               tgctTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGa                                                                                                       >  1:284982/1‑140 (MQ=255)

                                 ctTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGaaa                                                                                                     <  2:1047706/140‑1 (MQ=255)

                                            gATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCgaaga                                                                                          <  2:356483/140‑1 (MQ=255)

                                                tGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCgaaga                                                                                          >  1:1021604/1‑136 (MQ=255)

                                                tGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCgaaga                                                                                          <  2:1021604/136‑1 (MQ=255)

                                                    ttACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAg                                                                                  <  1:964810/140‑1 (MQ=255)

                                                     tACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCgaaga                                                                                          >  1:816949/1‑131 (MQ=255)

                                                     tACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCgaaga                                                                                          <  2:816949/131‑1 (MQ=255)

                                                     tACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGc                                                                                 >  1:379417/1‑140 (MQ=255)

                                                            agaAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGc                                                                          >  1:642712/1‑140 (MQ=255)

                                                            agaAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGc                                                                          >  2:804001/1‑140 (MQ=255)

                                                             gaAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCa                                                                         >  2:1052745/1‑140 (MQ=255)

                                                              aaaTACCTGGAAAATCCTCGCCGCGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATATGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCAt                                                                        <  1:629780/140‑1 (MQ=255)

                                                                  aCCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCAcc                                                                              >  1:516714/1‑130 (MQ=255)

                                                                  aCCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCAcc                                                                              <  2:516714/130‑1 (MQ=255)

                                                                  aCCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTAcc                                                                    >  1:893559/1‑140 (MQ=255)

                                                                              cTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGt                                                        <  1:589947/140‑1 (MQ=255)

                                                                                      gTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACAt                                                >  2:390564/1‑140 (MQ=255)

                                                                                                aGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGt                                      >  2:200614/1‑140 (MQ=255)

                                                                                                 ggTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGtt                                     >  2:679306/1‑140 (MQ=255)

                                                                                                     cTGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTgcgc                                 >  1:716631/1‑140 (MQ=255)

                                                                                                      tGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCt                                <  1:712219/140‑1 (MQ=255)

                                                                                                      tGGCTGACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCt                                <  1:679306/140‑1 (MQ=255)

                                                                                                           gACGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGc                           <  2:716631/140‑1 (MQ=255)

                                                                                                            aCGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCg                          >  1:454085/1‑140 (MQ=255)

                                                                                                            aCGGTCAGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCg                          >  2:242762/1‑140 (MQ=255)

                                                                                                                  aGGGCAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGtt                    >  2:482194/1‑140 (MQ=255)

                                                                                                                      cAACGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGGACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGata                >  1:1200111/1‑140 (MQ=255)

                                                                                                                        aCGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATc              <  2:379417/140‑1 (MQ=255)

                                                                                                                         cGCTATCTATCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCg             >  1:556786/1‑140 (MQ=255)

                                                                                                                           ctatctatCTGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGc           <  1:200614/140‑1 (MQ=255)

                                                                                                                              tctatctGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTAt        >  1:500231/1‑140 (MQ=255)

                                                                                                                              tctatctGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTAt        <  2:642712/140‑1 (MQ=255)

                                                                                                                                 atctGGTGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATcgc     <  1:645174/140‑1 (MQ=255)

                                                                                                                                    tggtggAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGt  <  1:390564/140‑1 (MQ=255)

CCATCTCCATGACCCGTGCGGAAGCGAAAGCTGCTTTCAGCAACGATATGGTTTACATGGAGAAATACCTGGAAAATCCTCGCCACGTCGAGATTCAGGTACTGGCTGACGGTCAGGGCAACGCTATCTATCTGGCGGAACGTGACTGCTCCATGCAACGCCGCCACCAGAAAGTGGTCGAAGAAGCGCCAGCACCGGGCATTACCCCGGAACTGCGTCGCTACATCGGCGAACGTTGCGCTAAAGCGTGTGTTGATATCGGCTATCGCGGT  >  NC_000913/3406458‑3406729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A