Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,472,700	T→C	N272S (AAC→AGC)	fusA ←	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,472,700	0	T	C	100.0%	205.4 / NA	58	N272S (AAC→AGC)	fusA	elongation factor G
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (32/26); total (32/26)

CTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGCTCTTCAGAAGCTTCA  >  NC_000913/3472570‑3472834

cTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACg                                                                                                                               <  2:112842/140‑1 (MQ=255)

cTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACg                                                                                                                               >  1:205684/1‑140 (MQ=255)

cTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACg                                                                                                                               <  1:310392/140‑1 (MQ=255)

cTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACg                                                                                                                               >  2:53524/1‑140 (MQ=255)

 tCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGc                                                                                                                              <  2:309305/140‑1 (MQ=255)

 tCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGc                                                                                                                              <  2:385261/140‑1 (MQ=255)

 tCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGc                                                                                                                              <  1:768847/140‑1 (MQ=255)

        caCTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACca                                                                                                                       <  2:505368/140‑1 (MQ=255)

        caCTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACca                                                                                                                       <  2:62706/140‑1 (MQ=255)

          cTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACcaca                                                                                                                     <  2:13807/140‑1 (MQ=255)

            tGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAgg                                                                                                                   <  1:393083/140‑1 (MQ=255)

                             gAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCg                                                                                                  >  2:122479/1‑140 (MQ=255)

                             gAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCg                                                                                                  <  2:98001/140‑1 (MQ=255)

                             gAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCg                                                                                                  <  1:712439/140‑1 (MQ=255)

                                  tCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGAtt                                                                                                     <  2:882132/132‑1 (MQ=255)

                                  tCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGAtt                                                                                                     >  1:882132/1‑132 (MQ=255)

                                           tcgtcCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAAcgcg                                                                                    <  2:205684/140‑1 (MQ=255)

                                            cgtcCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACTGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACgcgc                                                                                   >  2:579916/1‑140 (MQ=255)

                                            cgtcCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACgcgc                                                                                   >  2:720503/1‑140 (MQ=255)

                                            cgtcCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACgcgc                                                                                   >  2:166037/1‑140 (MQ=255)

                                            cgtcCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGCTACCAGGATGATTTCGTTGTTCAGAACgcgc                                                                                   >  2:946485/1‑140 (MQ=255)

                                                      aCCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCagag                                                                         <  2:670288/140‑1 (MQ=255)

                                                      aCCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCagag                                                                         <  1:305683/140‑1 (MQ=255)

                                                      aCCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCagag                                                                         <  1:656893/140‑1 (MQ=255)

                                                      aCCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCagag                                                                         <  2:659847/140‑1 (MQ=255)

                                                             aTCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCttt                                                                  >  1:310555/1‑140 (MQ=255)

                                                                  aGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCagag                                                                         <  1:880696/128‑1 (MQ=255)

                                                                  aGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCagag                                                                         >  2:880696/1‑128 (MQ=255)

                                                                  aGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGAttt                                                             >  2:991853/1‑140 (MQ=255)

                                                                         tCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTtc                                                      >  1:493058/1‑140 (MQ=255)

                                                                            aCCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTtcagt                                                   >  2:938234/1‑140 (MQ=255)

                                                                            aCCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTtcagt                                                   <  1:345231/140‑1 (MQ=255)

                                                                             ccGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTtcagtc                                                  <  1:938234/140‑1 (MQ=255)

                                                                               ggggATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTtcagt                                                   <  1:463472/137‑1 (MQ=255)

                                                                               ggggATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTtcagt                                                   >  2:463472/1‑137 (MQ=255)

                                                                                gggATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTcagtcagt                                               >  2:846436/1‑140 (MQ=255)

                                                                                   aTGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGttct                                            >  2:724448/1‑140 (MQ=255)

                                                                                   aTGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGttct                                            >  2:813453/1‑140 (MQ=255)

                                                                                      gCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTca                                         <  2:493058/140‑1 (MQ=255)

                                                                                                   tACCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTAtt                            >  1:953238/1‑140 (MQ=255)

                                                                                                    aCCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTAttt                           >  1:752435/1‑140 (MQ=255)

                                                                                                    aCCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTAttt                           >  2:343290/1‑140 (MQ=255)

                                                                                                    aCCGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTAttt                           >  1:57961/1‑140 (MQ=255)

                                                                                                     ccGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTAtttt                          >  1:207975/1‑140 (MQ=255)

                                                                                                      cGCATCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTAttttt                         >  1:1053136/1‑140 (MQ=255)

                                                                                                         aTCCAGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCa                      >  1:762979/1‑140 (MQ=255)

                                                                                                             aGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAg                  <  1:991853/140‑1 (MQ=255)

                                                                                                             aGCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAg                  <  2:762979/140‑1 (MQ=255)

                                                                                                              gCATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGc                 <  2:310555/140‑1 (MQ=255)

                                                                                                               cATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCAt                     <  2:824047/135‑1 (MQ=255)

                                                                                                               cATCGCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCAt                     >  1:824047/1‑135 (MQ=255)

                                                                                                                   gCCTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGCTCTTc            >  1:930169/1‑140 (MQ=255)

                                                                                                                    ccTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGCTCTTCa           >  2:448578/1‑140 (MQ=255)

                                                                                                                    ccTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGCTCTTCa           >  1:305596/1‑140 (MQ=255)

                                                                                                                    ccTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGCTCTTCa           >  2:900879/1‑140 (MQ=255)

                                                                                                                    ccTGAACACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGCTCTTCa           >  2:1256962/1‑140 (MQ=255)

                                                                                                                        aaCACCTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGCTCTTCAGAAg       >  1:1035822/1‑140 (MQ=255)

                                                                                                                             cTTTGCTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGCTCTTCAGAAGCTTCa  >  1:691330/1‑140 (MQ=255)

CTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGCTCTTCAGAAGCTTCA  >  NC_000913/3472570‑3472834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A