Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,577,042	A→G	V7A (GTT→GCT)	gntU ←	low‑affinity gluconate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,577,042	0	A	G	97.0%	222.2 / ‑2.1	66	V7A (GTT→GCT)	gntU	low‑affinity gluconate transporter
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (28/36); total (29/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

GTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTT  >  NC_000913/3576904‑3577176

gtcgCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAAc                                                                                                                                       >  1:1137447/1‑140 (MQ=255)

 tcgCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCa                                                                                                                                      >  2:450147/1‑140 (MQ=255)

   gCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAg                                                                                                                                    <  1:949462/140‑1 (MQ=255)

   gCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAg                                                                                                                                    <  2:622981/140‑1 (MQ=255)

   gCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAg                                                                                                                                    <  1:422638/140‑1 (MQ=255)

     tGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCg                                                                                                                                  <  2:250606/140‑1 (MQ=255)

       cGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGtt                                                                                                                                <  2:38717/140‑1 (MQ=255)

       cGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGtt                                                                                                                                <  1:450147/140‑1 (MQ=255)

             tATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTATAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTa                                                                                                                          <  2:1108829/140‑1 (MQ=255)

             tATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTa                                                                                                                          <  2:947621/140‑1 (MQ=255)

               tCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGt                                                                                                                        <  1:556200/140‑1 (MQ=255)

                    cGGCATACCAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCacta                                                                                                                   >  2:565925/1‑140 (MQ=255)

                            cAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTg                                                                                                           <  1:702934/140‑1 (MQ=255)

                               aaaaaaGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCt                                                                                                        <  1:610196/140‑1 (MQ=255)

                                aaaaaGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCtt                                                                                                       <  2:1137447/140‑1 (MQ=255)

                                     ggCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTta                                                                                                  <  1:821125/140‑1 (MQ=255)

                                       ccAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTtaat                                                                                                <  2:1155349/140‑1 (MQ=255)

                                         aGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTtaataa                                                                                              >  2:75880/1‑140 (MQ=255)

                                                 tGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATg                                                                                      <  2:585494/140‑1 (MQ=255)

                                                     caccacCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGc                                                                                  >  1:1004335/1‑140 (MQ=255)

                                                      accaccATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCt                                                                                 <  2:793272/140‑1 (MQ=255)

                                                      accaccATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCt                                                                                 <  2:1094963/140‑1 (MQ=255)

                                                      accaccATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCt                                                                                 <  2:1004335/140‑1 (MQ=255)

                                                        caccaTTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTg                                                                                   >  2:223311/1‑136 (MQ=255)

                                                        caccaTTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTg                                                                                   <  1:223311/136‑1 (MQ=255)

                                                        caccaTTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTg                                                                               >  1:978493/1‑140 (MQ=255)

                                                        caccaTTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTg                                                                               >  1:812929/1‑140 (MQ=255)

                                                        caccaTTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTg                                                                               >  1:1196904/1‑140 (MQ=255)

                                                         accaTTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGc                                                                              <  1:642274/140‑1 (MQ=255)

                                                               aaaGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCacaac                                                                        <  1:715761/140‑1 (MQ=255)

                                                                  gCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTAACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCca                                                                            >  1:914386/1‑133 (MQ=255)

                                                                  gCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTAACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCca                                                                            <  2:914386/133‑1 (MQ=255)

                                                                     aGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCacaa                                                                         <  2:293189/133‑1 (MQ=255)

                                                                     aGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCacaa                                                                         >  1:293189/1‑133 (MQ=255)

                                                                     aGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTc                                                                  <  2:1175940/140‑1 (MQ=255)

                                                                     aGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTc                                                                  >  2:534614/1‑140 (MQ=255)

                                                                     aGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTc                                                                  >  1:118679/1‑140 (MQ=255)

                                                                     aGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTc                                                                  <  2:978493/140‑1 (MQ=255)

                                                                     aGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTc                                                                  >  2:132321/1‑140 (MQ=255)

                                                                      ggAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTg                                                                                   >  2:785195/1‑122 (MQ=255)

                                                                      ggAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTg                                                                                   <  1:785195/122‑1 (MQ=255)

                                                                       gAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTc                                                                  >  1:372278/1‑138 (MQ=255)

                                                                       gAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTc                                                                  <  2:372278/138‑1 (MQ=255)

                                                                            cGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGt                                                           >  1:1079126/1‑140 (MQ=255)

                                                                            cGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGt                                                           >  2:99785/1‑140 (MQ=255)

                                                                             gTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGtt                                                          >  2:609586/1‑140 (MQ=255)

                                                                                cATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGAt                                                       <  2:193571/140‑1 (MQ=255)

                                                                                cATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGAt                                                       <  2:1079126/140‑1 (MQ=255)

                                                                                cATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGAt                                                       <  1:99785/140‑1 (MQ=255)

                                                                                      cgcCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGatat                                                 >  2:904462/1‑140 (MQ=255)

                                                                                        ccTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATcc                                               <  2:1061749/140‑1 (MQ=255)

                                                                                           tCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATccacc                                            <  2:899509/140‑1 (MQ=255)

                                                                                                aCTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAg                                       <  2:118679/140‑1 (MQ=255)

                                                                                                     aaaCAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACAt                                  <  1:534614/140‑1 (MQ=255)

                                                                                                         agcagcagTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATcc                                               >  2:569491/1‑123 (MQ=255)

                                                                                                         agcagcagTAAAACAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATcc                                               <  1:569491/123‑1 (MQ=255)

                                                                                                                   aaaCAGACCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCa                    >  1:1041936/1‑140 (MQ=255)

                                                                                                                         aCCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCAccc                 <  1:902643/137‑1 (MQ=255)

                                                                                                                         aCCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCAccc                 >  2:902643/1‑137 (MQ=255)

                                                                                                                         aCCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGc              <  1:609586/140‑1 (MQ=255)

                                                                                                                         aCCCTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGc              >  1:34489/1‑140 (MQ=255)

                                                                                                                           ccTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTc            >  2:590578/1‑140 (MQ=255)

                                                                                                                           ccTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTc            >  1:295277/1‑140 (MQ=255)

                                                                                                                           ccTACTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTc            >  1:256328/1‑140 (MQ=255)

                                                                                                                              aCTGCTGTTAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTg         >  2:990045/1‑140 (MQ=255)

                                                                                                                                     ttAAAGCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGtt  >  1:775971/1‑140 (MQ=255)

                                                                                                                                       aaagCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCAccc                 <  1:427444/123‑1 (MQ=255)

GTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTT  >  NC_000913/3576904‑3577176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A