Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,650,641	A→G	E135E (GAA→GAG)	arsC →	arsenate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,650,641	0	A	G	100.0%	129.5 / NA	37	E135E (GAA→GAG)	arsC	arsenate reductase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/20); total (17/20)

TTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAG  >  NC_000913/3650505‑3650772

ttCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCg                                                                                                                                  >  2:677140/1‑140 (MQ=255)

   tGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGt                                                                                                                               <  1:796249/140‑1 (MQ=255)

         aTCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAgcgc                                                                                                                         <  1:180316/140‑1 (MQ=255)

         aTCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAgcgc                                                                                                                         <  2:476418/140‑1 (MQ=255)

         aTCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCACAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAgcgc                                                                                                                         <  1:339896/140‑1 (MQ=255)

          tCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGTTGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGcc                                                                                                                        <  1:1096483/140‑1 (MQ=255)

          tCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAacggc                                                                                                                        >  2:61391/1‑138 (MQ=255)

                gATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAGGGcccggaaa                                                                                                                  >  1:517504/1‑134 (MQ=255)

                    gtggtgACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCCGAaaaaaaa                                                                                                              >  2:282456/1‑139 (MQ=255)

                          aCGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGa                                                                                                        <  2:826234/140‑1 (MQ=255)

                          aCGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGGAACGCCTGGAAAAAAgaggcgg                                                                                                        >  2:854890/1‑139 (MQ=255)

                             ccgcTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGatat                                                                                                     <  2:664083/140‑1 (MQ=255)

                               gcTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCCGAAAAAAAACGGCGATATcc                                                                                                   >  2:1145041/1‑140 (MQ=255)

                                      aCTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATATccccccaca                                                                                            <  1:976396/140‑4 (MQ=255)

                                           ccTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCTGAAAAAAAACGGCGGTATccccccccaggatg                                                                                       >  1:180869/1‑133 (MQ=255)

                                               tgCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAg                                                                                  >  1:479628/1‑140 (MQ=255)

                                               tgCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCTGGAATAAAACGGCGAAATTCCCCCC‑CAGGTTGTTAg                                                                                  >  1:790695/1‑140 (MQ=255)

                                               tgCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCCGAAAAAAAACGGGGGTATCCCCCCC‑CAGgttgtttg                                                                                  >  1:1078563/1‑138 (MQ=255)

                                                  cgccCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCTGAAATAAAACGGCGATATCCCCCCC‑CCGGGTGTTAGaaa                                                                               >  1:88796/1‑140 (MQ=255)

                                                    cccTTCAGAAGTGGTGCTAGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCCGAAATAAAACGGCGGTATCCCCCCC‑CAGGGTGTTAGaaaaa                                                                             >  1:143547/1‑139 (MQ=255)

                                                      cTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTg                                                                           <  2:739533/140‑1 (MQ=255)

                                                         cAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAATAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTgcgc                                                                        <  2:869794/140‑1 (MQ=255)

                                                         cAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTgcgc                                                                        <  1:282456/140‑1 (MQ=255)

                                                         cAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCTGAAAAAAAAAGGGGGGATCCCCCCC‑CAGGGTTTTTGAAAAATgggc                                                                        >  1:925704/1‑140 (MQ=255)

                                                                ggtgCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCtttattt                                                                 <  2:647459/140‑1 (MQ=255)

                                                                 gtgCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAACGCCCGGAATAAAACGGCGATATTCCCCCC‑CCGGGTGTTTGAAAAAAGGGCtttttttt                                                                >  2:204943/1‑139 (MQ=255)

                                                                                               agacagttCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATcc                                  <  1:1012713/134‑1 (MQ=255)

                                                                                                  gcATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGAc                               <  2:479628/140‑1 (MQ=255)

                                                                                                        tcCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACaa                         <  1:1145041/140‑1 (MQ=255)

                                                                                                                aGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCCGAAATAAAAAGGCGATATCCCCCCC‑CAGGGTGTTAGAAAAAAGCGCTTTTTTTATTTCGGGTGCGACGCTGGCGCGGCTTATCCCACCTACaaaactgtct                 >  2:1012713/1‑133 (MQ=255)

                                                                                                                  aTGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCCGGAATAAAACGGCGGGATCCCCCCC‑CCGGGTGTTTGAAAAAAGCGCTTTATTTTTGTTGGGTGCGACGCCGGCGCGTCTTTTTCCACCCACAAAATTATTaaa               >  2:984021/1‑140 (MQ=255)

                                                                                                                    ggCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCTGAAAAAAAACGGCGATATCCCCCCC‑CCGGGTGTTAGAAAAAAGGGGTTTTTTTTTTTTGGATGCGGCGCTGGCGCGTCTTTTCCCACCTACAAAATTATGaaaaa             >  2:342502/1‑139 (MQ=255)

                                                                                                                       gagaAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGGCGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATCCg          <  2:262222/140‑1 (MQ=39)

                                                                                                                           aaGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGa      <  2:519734/140‑1 (MQ=255)

                                                                                                                            aGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGaa     <  2:925704/140‑1 (MQ=255)

                                                                                                                             gTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAAt    <  1:210961/140‑1 (MQ=255)

                                                                                                                             gTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAAt    <  2:180869/140‑1 (MQ=255)

                                                                                                                              tGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATa   <  2:88796/140‑1 (MQ=255)

                                                                                                                               ggTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAg  <  1:643464/140‑1 (MQ=255)

TTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAG  >  NC_000913/3650505‑3650772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A