Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,650,680	(C)_7→6	intergenic (+18/‑611)	arsC → / → yhiS	arsenate reductase/putative uncharacterized protein YhiS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,650,674	0	C	.	100.0%	99.5 / NA	24	intergenic (+12/‑617)	arsC/yhiS	arsenate reductase/putative uncharacterized protein YhiS
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (5/19); total (5/19)

GCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAGTATGTTATTTGTAGGCCTGACAGGCGTAGCGCATCA  >  NC_000913/3650536‑3650808

gcTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCCGAAAAAAAACGGCGATATcc                                                                                                                                       >  2:1145041/1‑140 (MQ=255)

       aCTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATATccccccaca                                                                                                                                <  1:976396/140‑4 (MQ=255)

            ccTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCTGAAAAAAAACGGCGGTATccccccccaggatg                                                                                                                           >  1:180869/1‑133 (MQ=255)

                tgCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAg                                                                                                                      >  1:479628/1‑140 (MQ=255)

                tgCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCTGGAATAAAACGGCGAAATTCCCCCC‑CAGGTTGTTAg                                                                                                                      >  1:790695/1‑140 (MQ=255)

                tgCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCCGAAAAAAAACGGGGGTATCCCCCCC‑CAGgttgtttg                                                                                                                      >  1:1078563/1‑138 (MQ=255)

                   cgccCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCTGAAATAAAACGGCGATATCCCCCCC‑CCGGGTGTTAGaaa                                                                                                                   >  1:88796/1‑140 (MQ=255)

                     cccTTCAGAAGTGGTGCTAGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCCGAAATAAAACGGCGGTATCCCCCCC‑CAGGGTGTTAGaaaaa                                                                                                                 >  1:143547/1‑139 (MQ=255)

                       cTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTg                                                                                                               <  2:739533/140‑1 (MQ=255)

                          cAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAATAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTgcgc                                                                                                            <  2:869794/140‑1 (MQ=255)

                          cAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTgcgc                                                                                                            <  1:282456/140‑1 (MQ=255)

                          cAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCTGAAAAAAAAAGGGGGGATCCCCCCC‑CAGGGTTTTTGAAAAATgggc                                                                                                            >  1:925704/1‑140 (MQ=255)

                                 ggtgCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCtttattt                                                                                                     <  2:647459/140‑1 (MQ=255)

                                  gtgCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAACGCCCGGAATAAAACGGCGATATTCCCCCC‑CCGGGTGTTTGAAAAAAGGGCtttttttt                                                                                                    >  2:204943/1‑139 (MQ=255)

                                                                agacagttCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATcc                                                                      <  1:1012713/134‑1 (MQ=255)

                                                                   gcATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGAc                                                                   <  2:479628/140‑1 (MQ=255)

                                                                         tcCAAGGAAGATGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACaa                                                             <  1:1145041/140‑1 (MQ=255)

                                                                                 aGATGGCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCCGAAATAAAAAGGCGATATCCCCCCC‑CAGGGTGTTAGAAAAAAGCGCTTTTTTTATTTCGGGTGCGACGCTGGCGCGGCTTATCCCACCTACaaaactgtct                                                     >  2:1012713/1‑133 (MQ=255)

                                                                                   aTGGCGAGAAAGTGGTTGATGAGGCGGGTAAGCGCCCGGAATAAAACGGCGGGATCCCCCCC‑CCGGGTGTTTGAAAAAAGCGCTTTATTTTTGTTGGGTGCGACGCCGGCGCGTCTTTTTCCACCCACAAAATTATTaaa                                                   >  2:984021/1‑140 (MQ=255)

                                                                                     ggCGAGAAAGTGGTTGATGAGGCGGGGAAGCGCCTGAAAAAAAACGGCGATATCCCCCCC‑CCGGGTGTTAGAAAAAAGGGGTTTTTTTTTTTTGGATGCGGCGCTGGCGCGTCTTTTCCCACCTACAAAATTATGaaaaa                                                 >  2:342502/1‑139 (MQ=255)

                                                                                        gagaAAGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGGCGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATCCg                                              <  2:262222/140‑1 (MQ=39)

                                                                                            aaGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGa                                          <  2:519734/140‑1 (MQ=255)

                                                                                             aGTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGaa                                         <  2:925704/140‑1 (MQ=255)

                                                                                              gTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAAt                                        <  1:210961/140‑1 (MQ=255)

                                                                                              gTGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAAt                                        <  2:180869/140‑1 (MQ=255)

                                                                                               tGGTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATa                                       <  2:88796/140‑1 (MQ=255)

                                                                                                ggTTGATGAGGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAg                                      <  1:643464/140‑1 (MQ=255)

                                                                                                          gCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAGTATGTTAttt                            <  2:143547/140‑1 (MQ=255)

                                                                                                               tAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAGTATGTTATTCGTAgg                       <  2:1078563/140‑1 (MQ=255)

                                                                                                                    gcCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAGTATGTTATTTGTAGGCCTGa                  >  2:366550/1‑140 (MQ=255)

                                                                                                                    gcCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAGTATGTTATTTGTAGGCCTGa                  <  1:1246194/140‑1 (MQ=255)

                                                                                                                         aaaTAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAGTATGTTATTTGTAGGCCTGACAGGc             >  2:213321/1‑140 (MQ=255)

                                                                                                                             aaaGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAGTATGTTATTTGTAGGCCTGACAGGCGTAg         <  1:984021/140‑1 (MQ=255)

                                                                                                                               aGCGGCGATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAGTATGTTATTTGTAGGCCTGACAGGCGTAgcg       <  1:935022/140‑1 (MQ=255)

                                                                                                                                 cggcgATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAGTATGTTATTTGTAGGCCTGACAGGCGTAGCGCa     <  2:517504/140‑1 (MQ=255)

                                                                                                                                  ggcgATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAGTATGTTATTTGTAGGCCTGACAGGCGTAGCGCAt    <  1:677140/140‑1 (MQ=255)

                                                                                                                                   gcgATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAGTATGTTATTTGTAGGCCTGACAGGCGTAGCGCATc   >  2:31660/1‑140 (MQ=255)

                                                                                                                                   gcgATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAGTATGTTATTTGTAGGCCTGACAGGCGTAGCGCATc   >  2:815818/1‑140 (MQ=255)

                                                                                                                                    cgATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAGTATGTTATTTGTAGGCCTGACAGGCGTAGCGCATCa  <  1:31660/140‑1 (MQ=255)

                                                                                                                                    cgATAT‑CCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAGTATGTTATTTGTAGGCCTGACAGGCGTAGCGCATCa  >  2:1166519/1‑140 (MQ=255)

GCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAGAAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACAAAGTTATGAAAATTCGATGAATAGTATGTTATTTGTAGGCCTGACAGGCGTAGCGCATCA  >  NC_000913/3650536‑3650808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A