Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,748,407	A→G	T442A (ACC→GCC)	lyxK →	L‑xylulose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,748,407	0	A	G	100.0%	207.4 / NA	60	T442A (ACC→GCC)	lyxK	L‑xylulose kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/32); total (28/32)

CCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCA  >  NC_000913/3748271‑3748538

ccTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCg                                                                                                                                  >  2:558298/1‑140 (MQ=255)

   aCGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCgggg                                                                                                                               >  2:477207/1‑140 (MQ=255)

   aCGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCgggg                                                                                                                               >  2:475210/1‑140 (MQ=255)

        tCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTAt                                                                                                                          >  2:271573/1‑140 (MQ=255)

          aCTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATca                                                                                                                        <  1:874049/140‑1 (MQ=255)

          aCTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATca                                                                                                                        >  2:981900/1‑140 (MQ=255)

          aCTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATca                                                                                                                        >  2:915571/1‑140 (MQ=255)

          aCTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATca                                                                                                                        >  2:847429/1‑140 (MQ=255)

          aCTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATca                                                                                                                        >  1:106918/1‑140 (MQ=255)

           cTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATcac                                                                                                                       <  1:1240603/140‑1 (MQ=255)

                ggcCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACtt                                                                                                                  <  2:322336/140‑1 (MQ=255)

                      gcgcACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGa                                                                                                            >  1:427323/1‑140 (MQ=255)

                                  gTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGa                                                                                                <  2:427323/140‑1 (MQ=255)

                                   tCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGAc                                                                                               <  2:528097/140‑1 (MQ=255)

                                    cTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACt                                                                                              >  2:754508/1‑140 (MQ=255)

                                     tGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACtt                                                                                             >  2:588081/1‑140 (MQ=255)

                                     tGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACtt                                                                                             >  2:231494/1‑140 (MQ=255)

                                     tGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACtt                                                                                             >  1:522666/1‑140 (MQ=255)

                                        aTGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCGGCGAAGCCCAACGTGACTTGCg                                                                                          >  2:1214392/1‑140 (MQ=255)

                                            aaaTGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGacac                                                                                      <  1:765890/140‑1 (MQ=255)

                                             aaTGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACAcc                                                                                     <  2:736498/140‑1 (MQ=255)

                                             aaTGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACAcc                                                                                     <  2:466549/140‑1 (MQ=255)

                                             aaTGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACAcc                                                                                     >  1:350428/1‑140 (MQ=255)

                                             aaTGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACAcc                                                                                     >  1:736498/1‑140 (MQ=255)

                                              aTGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACAccc                                                                                    <  1:588081/140‑1 (MQ=255)

                                               tGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCg                                                                                   >  1:809111/1‑140 (MQ=255)

                                                 cTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCC‑GGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTg                                                                                >  1:627888/1‑140 (MQ=255)

                                                   ggCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGt                                                                                 >  1:572268/1‑138 (MQ=255)

                                                   ggCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGt                                                                                 <  2:572268/138‑1 (MQ=255)

                                                    gCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTgcg                                                                              <  1:111763/140‑1 (MQ=255)

                                                        aCGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCAcc                                                                          <  1:271573/140‑1 (MQ=255)

                                                        aCGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCAcc                                                                          <  2:809111/140‑1 (MQ=255)

                                                           tCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCctg                                                                       <  1:915571/140‑1 (MQ=255)

                                                            cAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCctgc                                                                      <  1:847429/140‑1 (MQ=255)

                                                              gCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCctgctg                                                                    <  1:1198199/140‑1 (MQ=255)

                                                              gCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCctgctg                                                                    <  2:522666/140‑1 (MQ=255)

                                                               cGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCtgctgc                                                                   <  2:848992/140‑1 (MQ=255)

                                                               cGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCtgctgc                                                                   <  1:1206086/140‑1 (MQ=255)

                                                                      cGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGatat                                                            <  1:754508/140‑1 (MQ=255)

                                                                         aTCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGAc                                                         >  1:648485/1‑140 (MQ=255)

                                                                              gCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCGGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGccc                                                    <  1:1214392/140‑1 (MQ=255)

                                                                              gCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGccc                                                    <  1:309288/140‑1 (MQ=255)

                                                                              gCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGccc                                                    >  1:419353/1‑140 (MQ=255)

                                                                              gCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCAGTGCGCACCCTGCTGCCAGATATGACCGccc                                                    <  2:315772/140‑1 (MQ=255)

                                                                               cTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCa                                                   <  2:648485/140‑1 (MQ=255)

                                                                                 gCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCAtg                                                 >  1:434622/1‑139 (MQ=255)

                                                                                  ccGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCa                                                >  1:948475/1‑140 (MQ=255)

                                                                                                aaCCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCaaaaaa                                  <  2:350428/140‑1 (MQ=255)

                                                                                                     gctgctTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAatat                             <  2:948475/140‑1 (MQ=255)

                                                                                                      ctgctTTGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATc                            >  1:638765/1‑140 (MQ=255)

                                                                                                           ttGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGt                       <  1:1207142/140‑1 (MQ=255)

                                                                                                            tGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGtt                      <  1:477207/140‑1 (MQ=255)

                                                                                                            tGGTGCGGCCCTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGtt                      <  2:1235188/140‑1 (MQ=255)

                                                                                                                    cccTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCAt              <  1:1130608/140‑1 (MQ=255)

                                                                                                                      cTTGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCAtct            <  1:475210/140‑1 (MQ=255)

                                                                                                                        tGCCGCCCGCGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAGCGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCa          >  1:1210763/1‑140 (MQ=255)

                                                                                                                              ccgcGTCGGCGCCGTGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCg    <  1:558298/140‑1 (MQ=255)

                                                                                                                              ccgcGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCg    <  1:231494/140‑1 (MQ=255)

                                                                                                                              ccgcGTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCg    >  2:1222065/1‑140 (MQ=255)

                                                                                                                                gcgTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCa  >  2:148998/1‑140 (MQ=255)

                                                                                                                                gcgTCGGCGCCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCa  >  2:1028583/1‑140 (MQ=255)

CCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCA  >  NC_000913/3748271‑3748538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A