Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,787,890	T→C	G351G (GGT→GGC)	envC →	murein hydrolase activator EnvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,787,890	0	T	C	100.0%	162.9 / NA	48	G351G (GGT→GGC)	envC	murein hydrolase activator EnvC
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/21); total (27/21)

GCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGG  >  NC_000913/3787758‑3788024

gCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCtggtgg                                                                                                                                 <  1:1255453/140‑1 (MQ=255)

  aTCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCtggtggtg                                                                                                                               <  2:956852/140‑1 (MQ=255)

  aTCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCtggtggtg                                                                                                                               <  1:1145638/140‑1 (MQ=255)

        aTGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGGAAGGCTACGGCCTGGTGGTGGTGGtt                                                                                                                         <  2:994850/140‑1 (MQ=255)

        aTGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGtt                                                                                                                         <  1:1095330/140‑1 (MQ=255)

                         ggTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGa                                                                                                        >  2:321434/1‑140 (MQ=255)

                          gTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGAc                                                                                                       >  1:222527/1‑140 (MQ=255)

                           tGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACa                                                                                                      <  2:124556/140‑1 (MQ=255)

                             aaTTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATg                                                                                                    >  2:104161/1‑140 (MQ=255)

                                tACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGCACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGt                                                                                                 >  2:704088/1‑140 (MQ=255)

                                   gCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATg                                                                                                    <  1:204102/134‑1 (MQ=255)

                                   gCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATg                                                                                                    >  2:204102/1‑134 (MQ=255)

                                     tGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTa                                                                                            <  1:36401/140‑1 (MQ=255)

                                     tGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTa                                                                                            <  1:1024236/140‑1 (MQ=255)

                                          aGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCt                                                                                       <  1:751681/140‑1 (MQ=255)

                                              aTGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATaa                                                                                   <  2:186822/140‑1 (MQ=255)

                                                    aTCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCagag                                                                             >  1:322918/1‑140 (MQ=255)

                                                           cTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTg                                                                      >  1:142200/1‑140 (MQ=255)

                                                            ttCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTgg                                                                     >  1:529514/1‑140 (MQ=255)

                                                                   ggTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGt                                                              >  2:1161784/1‑140 (MQ=255)

                                                                      aCTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAGGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTgg                                                           >  2:338199/1‑140 (MQ=255)

                                                                      aCTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTgg                                                           >  1:300507/1‑140 (MQ=255)

                                                                      aCTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTgg                                                           >  2:528204/1‑140 (MQ=255)

                                                                      aCTGAAGTTAAAGCGATTGCCGACGGCCGGG‑GATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGAGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGt                                                          >  2:218185/1‑140 (MQ=255)

                                                                        tGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGt                                                                    >  2:1064952/1‑129 (MQ=255)

                                                                        tGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGt                                                                    <  1:1064952/129‑1 (MQ=255)

                                                                        tGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGtt                                                         >  2:112167/1‑140 (MQ=255)

                                                                        tGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGtt                                                         >  1:240356/1‑140 (MQ=255)

                                                                        tGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTAGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGtt                                                         >  2:1070917/1‑140 (MQ=255)

                                                                               aaaGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGt                                                  >  2:1133356/1‑140 (MQ=255)

                                                                               aaaGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGt                                                  >  2:851862/1‑140 (MQ=255)

                                                                                 aGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTc                                                >  2:1212390/1‑140 (MQ=255)

                                                                                    gATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTcgcg                                             >  1:1238145/1‑140 (MQ=255)

                                                                                    gATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCAGGTGGTGGTCGTTGAGCATGGTAAAGGTGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTcgcg                                             <  2:1122338/140‑1 (MQ=255)

                                                                                      ttGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTcgcgcg                                           <  1:104161/140‑1 (MQ=255)

                                                                                           gACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCa                                      <  1:331784/140‑1 (MQ=255)

                                                                                              ggTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGcc                                   <  1:851862/140‑1 (MQ=255)

                                                                                                     tGATTCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCa                            <  2:240356/140‑1 (MQ=255)

                                                                                                        ttCTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACtg                         >  1:214103/1‑140 (MQ=255)

                                                                                                          cTGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACtggt                       >  2:297140/1‑140 (MQ=255)

                                                                                                           tGGCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACtggtg                      <  2:214103/140‑1 (MQ=255)

                                                                                                             gCTGACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTggg                    >  1:1245563/1‑140 (MQ=255)

                                                                                                                gACTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGgcag                 <  1:1161784/140‑1 (MQ=255)

                                                                                                                  cTGGCTGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGgcagca               >  1:1186821/1‑140 (MQ=255)

                                                                                                                       tGCAAGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGc          >  2:310629/1‑140 (MQ=255)

                                                                                                                          aaGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGt       <  1:1225161/140‑1 (MQ=255)

                                                                                                                           aGGCTACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTc      <  2:529514/140‑1 (MQ=255)

                                                                                                                               tACGGCCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAggg  >  2:653725/1‑140 (MQ=255)

GCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGG  >  NC_000913/3787758‑3788024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A