Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 260 73.5 2514320 99.3% 2496719 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,852,535 T→C S84G (AGC→GGC)  ilvB ← acetohydroxy acid synthase I subunit IlvB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,852,5350TC100.0% 250.9 / NA 71S84G (AGC→GGC) ilvBacetohydroxy acid synthase I subunit IlvB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/49);  total (22/49)

CCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGG  >  NC_000913/3852399‑3852668
                                                                                                                                        |                                                                                                                                      
ccGTGGGTGTCCACTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCcaca                                                                                                                                     <  1:929761/140‑1 (MQ=255)
 cGTAGGTGTCCACTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAg                                                                                                                                    <  1:354319/140‑1 (MQ=255)
   tAGGTGTCCACTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGc                                                                                                                                  >  1:217472/1‑140 (MQ=255)
         tCCACTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGGCACCAGGGTAGTCGCACCCCGTCCCCCACAGGCCATACa                                                                                                                            >  2:929761/1‑140 (MQ=255)
            ccTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGAc                                                                                                                         <  2:311759/139‑1 (MQ=255)
                        gCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGGCACCCGGGTAGTCGCACCCCGGCCGCCACAGGCCCTACAAAACG‑CCGGGTTTcc                                                                                                            >  1:287811/1‑140 (MQ=255)
                        gCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGGCACCAGGGTAGTCGCCCCCCGTCCGCCACAGGCCATACAAACCG‑CCGGGTTAcc                                                                                                            >  2:1062546/1‑140 (MQ=255)
                        gCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGGCACCAGGGTAGTCGCACCCGGGCCCCCCCAGGCCATACAGACCC‑CCGGGTTAcc                                                                                                            >  1:1200131/1‑140 (MQ=255)
                          gTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGGTGGAGTCCAGCCGCGCATCGGCAATGGCGGGCACCAGGGTAGTCGCACCCGGGCCGCCCCAAGGCCTACAGACCGCCCGGTTTcccc                                                                                                           >  2:174319/1‑139 (MQ=255)
                          gTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGGTAGTCGCACCCGGTCCGCCACAGGCCATACAAACCG‑CCGGTTTACCGt                                                                                                          >  1:127213/1‑140 (MQ=255)
                               tGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTg                                                                                                     <  2:196268/140‑1 (MQ=255)
                                gCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTgc                                                                                                    <  2:217472/140‑1 (MQ=255)
                                gCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTgc                                                                                                    <  1:1204478/140‑1 (MQ=255)
                                gCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTgc                                                                                                    <  2:757982/140‑1 (MQ=255)
                                gCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTgc                                                                                                    <  1:79380/140‑1 (MQ=255)
                                   gATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTgcgcg                                                                                                 <  1:613515/140‑1 (MQ=255)
                                   gATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTgcgcg                                                                                                 <  1:244610/140‑1 (MQ=255)
                                              cGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGa                                                                                      <  1:1005215/140‑1 (MQ=255)
                                               gggACCCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAg                                                                                     <  2:237589/140‑1 (MQ=255)
                                               gggAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGGCCCCAGGGTAGTCGCACCCCGGCCGCCACAGGCCATACAGACCG‑CCGGGTTACCGTCGGGGCGCGCCCTTCCCTGac                                                                                     >  2:244610/1‑139 (MQ=255)
                                                 gAACCTTAACAGTTATGCAAATCAACGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAAGGTAGTCGCACCCCGGCCGCCACAGGCCATACAGACCG‑CCGGTTTTCCGCCGGTGCGCGCCCTTTCCCGAGCg                                                                                   >  2:229752/1‑140 (MQ=255)
                                                 gAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCCCGCATCGGCAATGGCGGTCACCAAGGTAGTCGCACCCGGGCCCCCCCCGGGCCTACAGACCC‑CCGGGTTTCCGTCGGTGCGCGCCCCTCCCTGAgag                                                                                   >  2:11153/1‑138 (MQ=255)
                                                     cTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATaa                                                                               <  2:568471/140‑1 (MQ=255)
                                                        aCCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGc                                                                            <  1:753572/140‑1 (MQ=255)
                                                           aGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCcg                                                                         <  1:174319/140‑1 (MQ=255)
                                                             tGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCcgcg                                                                       <  2:800644/140‑1 (MQ=255)
                                                             tGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCcgcg                                                                       <  1:845423/140‑1 (MQ=255)
                                                             tGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCcgcg                                                                       <  1:682611/140‑1 (MQ=255)
                                                              gATGCAAATCAGCGGGATGGAGACCAGCGGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGCCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCgcgc                                                                      <  2:227325/140‑1 (MQ=255)
                                                               aTGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGcc                                                                     <  1:450119/140‑1 (MQ=255)
                                                               aTGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGcc                                                                     <  2:797925/140‑1 (MQ=255)
                                                               aTGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGcc                                                                     >  1:123253/1‑140 (MQ=255)
                                                               aTGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGcc                                                                     >  2:687881/1‑140 (MQ=255)
                                                               aTGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGcc                                                                     >  1:527128/1‑140 (MQ=255)
                                                               aTGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGcc                                                                     <  1:449515/140‑1 (MQ=255)
                                                                  cAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTg                                                                  >  1:887409/1‑140 (MQ=255)
                                                                  cAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTg                                                                  >  1:1018954/1‑140 (MQ=255)
                                                                       cAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCAt                                                             >  2:213078/1‑140 (MQ=255)
                                                                       cAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCAt                                                             >  2:936544/1‑140 (MQ=255)
                                                                         gCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGa                                                           <  2:1114785/140‑1 (MQ=255)
                                                                          cGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGAc                                                          >  2:931346/1‑140 (MQ=255)
                                                                          cGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGAc                                                          >  2:1079582/1‑140 (MQ=255)
                                                                           gggATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACg                                                         <  1:213078/140‑1 (MQ=255)
                                                                           gggATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACg                                                         <  2:243671/140‑1 (MQ=255)
                                                                              aTGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGc                                                      <  1:486008/140‑1 (MQ=255)
                                                                                     ccAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAata                                               <  2:16505/140‑1 (MQ=255)
                                                                                       aGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATg                                             <  1:725006/140‑1 (MQ=255)
                                                                                       aGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATg                                             >  2:125364/1‑140 (MQ=255)
                                                                                            cgcATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGa                                        >  2:826209/1‑140 (MQ=255)
                                                                                            cgcATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGa                                        >  2:439686/1‑140 (MQ=255)
                                                                                                  ggCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCg                                  <  2:1200131/140‑1 (MQ=255)
                                                                                                  ggCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCg                                  <  2:287811/140‑1 (MQ=255)
                                                                                                  ggCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCg                                  <  2:105259/140‑1 (MQ=255)
                                                                                                  ggCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCg                                  <  2:459679/140‑1 (MQ=255)
                                                                                                      aTGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCt                              <  1:1027093/140‑1 (MQ=255)
                                                                                                      aTGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCt                              <  2:882774/140‑1 (MQ=255)
                                                                                                      aTGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCt                              <  1:89111/140‑1 (MQ=255)
                                                                                                       tGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCtt                             <  1:399676/140‑1 (MQ=255)
                                                                                                       tGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCACGACGGGCCAGAATATGGCGGATTTGCGTGCtt                             <  1:601154/140‑1 (MQ=255)
                                                                                                        ggCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCttt                            >  2:1021301/1‑140 (MQ=255)
                                                                                                         gCGGTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTg                           >  1:931464/1‑140 (MQ=255)
                                                                                                            gTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCt                        <  2:1018954/140‑1 (MQ=255)
                                                                                                            gTCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGAATTTTGTGCTTTTGCt                        >  2:89111/1‑140 (MQ=255)
                                                                                                             tCACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTTCGTGCTTTGGCtt                       >  1:680459/1‑140 (MQ=255)
                                                                                                              cACCAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTa                      <  2:651715/140‑1 (MQ=255)
                                                                                                                ccAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAg                    <  1:1062546/140‑1 (MQ=255)
                                                                                                                ccAGGTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAg                    <  1:704010/140‑1 (MQ=255)
                                                                                                                   ggTTAGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCa                 >  1:177661/1‑140 (MQ=255)
                                                                                                                       aGTCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGt             >  1:1111249/1‑140 (MQ=255)
                                                                                                                         tCGCACCCGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTaa           >  1:656920/1‑140 (MQ=255)
                                                                                                                              cccGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcagg      <  1:931346/140‑1 (MQ=255)
                                                                                                                              cccGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcagg      <  2:931464/140‑1 (MQ=255)
                                                                                                                              cccGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcagg      <  1:1079582/140‑1 (MQ=255)
                                                                                                                              cccGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcagg      <  1:1021301/140‑1 (MQ=255)
                                                                                                                              cccGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcagg      <  2:520087/140‑1 (MQ=255)
                                                                                                                              cccGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcagg      <  1:687881/140‑1 (MQ=255)
                                                                                                                              cccGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcagg      <  2:177661/140‑1 (MQ=255)
                                                                                                                              cccGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcagg      <  2:160792/140‑1 (MQ=255)
                                                                                                                              cccGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcagg      <  2:1004667/140‑1 (MQ=255)
                                                                                                                                cGGTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcaggca    <  1:936544/140‑1 (MQ=255)
                                                                                                                                  gTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTTGCTTAAGGCATCGTAAAcaggcagg  >  1:584411/1‑140 (MQ=255)
                                                                                                                                  gTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcaggcagg  >  1:371321/1‑140 (MQ=255)
                                                                                                                                  gTCCGCCACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcaggcagg  >  1:871504/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                      
CCGTAGGTGTCCACTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCG‑CCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGG  >  NC_000913/3852399‑3852668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A