Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,862,224	G→A	V83V (GTC→GTT)	glvB ←	putative PTS enzyme II component GlvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,862,224	0	G	A	100.0%	257.3 / NA	74	V83V (GTC→GTT)	glvB	putative PTS enzyme II component GlvB
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (36/38); total (36/38)

CCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTCACGTCCCGGC  >  NC_000913/3862088‑3862359

ccTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAAcc                                                                                                                                      <  1:759461/140‑1 (MQ=255)

ccTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAAcc                                                                                                                                      <  2:1252880/140‑1 (MQ=255)

    aaTGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGc                                                                                                                                  >  1:1092049/1‑140 (MQ=255)

     aTGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGcc                                                                                                                                 <  2:1183117/140‑1 (MQ=255)

     aTGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGcc                                                                                                                                 >  1:1199851/1‑140 (MQ=255)

      tGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGccc                                                                                                                                <  1:652231/140‑1 (MQ=255)

         cATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAgg                                                                                                                             <  1:71920/140‑1 (MQ=255)

         cATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAgg                                                                                                                             >  2:458471/1‑140 (MQ=255)

         cATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAgg                                                                                                                             >  1:1069306/1‑140 (MQ=255)

         cATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAgg                                                                                                                             <  1:1148196/140‑1 (MQ=255)

         cATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAgg                                                                                                                             >  1:38662/1‑140 (MQ=255)

           tCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGc                                                                                                                           >  2:293923/1‑140 (MQ=255)

           tCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACCTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGc                                                                                                                           <  2:1227061/140‑1 (MQ=255)

              ccACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTg                                                                                                                        <  2:1092049/140‑1 (MQ=255)

              ccACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTg                                                                                                                        >  1:182364/1‑140 (MQ=255)

              ccACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGAAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTg                                                                                                                        >  2:988643/1‑140 (MQ=255)

               cACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGc                                                                                                                       <  2:182364/140‑1 (MQ=255)

                   aCGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGa                                                                                                                   >  2:603665/1‑140 (MQ=255)

                   aCGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGa                                                                                                                   <  2:765416/140‑1 (MQ=255)

                     ggAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATa                                                                                                                 <  2:1199851/140‑1 (MQ=255)

                         gACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCgg                                                                                                             >  1:168797/1‑140 (MQ=255)

                         gACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCgg                                                                                                             <  2:38662/140‑1 (MQ=255)

                            gCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAg                                                                                                          >  2:189536/1‑140 (MQ=255)

                             ccGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGc                                                                                                         <  1:382792/140‑1 (MQ=255)

                             ccGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGc                                                                                                         <  1:1132799/140‑1 (MQ=255)

                              cGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGcc                                                                                                        <  1:851145/140‑1 (MQ=255)

                                                  aaGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTgg                                                                                    <  2:581137/140‑1 (MQ=255)

                                                       ttCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCATGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTg                                                                               <  1:831911/140‑1 (MQ=255)

                                                        tCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGc                                                                              >  1:1204465/1‑140 (MQ=255)

                                                            cATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCcgcg                                                                          <  2:763134/140‑1 (MQ=255)

                                                                   cGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGttt                                                                   >  1:475141/1‑140 (MQ=255)

                                                                            cATGTCATGAAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTcgcg                                                          <  1:988643/140‑1 (MQ=255)

                                                                              tGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAg                                                        <  2:475141/140‑1 (MQ=255)

                                                                               gTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAgg                                                       >  2:723526/1‑140 (MQ=255)

                                                                               gTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAgg                                                       >  2:806087/1‑140 (MQ=255)

                                                                                 cATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCt                                                     <  1:1040110/140‑1 (MQ=255)

                                                                                 cATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCt                                                     <  1:389113/140‑1 (MQ=255)

                                                                                 cATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCt                                                     <  1:577265/140‑1 (MQ=255)

                                                                                   tGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCttt                                                   >  1:332245/1‑140 (MQ=255)

                                                                                   tGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCttt                                                   >  2:820879/1‑140 (MQ=255)

                                                                                        tGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTAtt                                              >  2:662365/1‑140 (MQ=255)

                                                                                        tGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCA‑GGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTCTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTc                                             >  2:1168304/1‑140 (MQ=255)

                                                                                         gCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTc                                             <  2:1204465/140‑1 (MQ=255)

                                                                                          cAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCg                                            >  2:100824/1‑140 (MQ=255)

                                                                                          cAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCg                                            >  1:654134/1‑140 (MQ=255)

                                                                                          cAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCg                                            >  1:557015/1‑140 (MQ=255)

                                                                                          cAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCg                                            >  2:165907/1‑140 (MQ=255)

                                                                                           aaTACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCgg                                           <  1:1094942/140‑1 (MQ=255)

                                                                                               cGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATTCCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCttt                                       >  1:1096996/1‑140 (MQ=255)

                                                                                               cGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCttt                                       >  1:1037561/1‑140 (MQ=255)

                                                                                                  aaaCGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGa                                    >  2:168126/1‑140 (MQ=255)

                                                                                                    aCGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCt                                         >  1:1243434/1‑133 (MQ=255)

                                                                                                    aCGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTGTGTATTCGGCt                                         <  2:1243434/133‑1 (MQ=255)

                                                                                                       cgTCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTaga                               >  2:235273/1‑140 (MQ=255)

                                                                                                         tCGCGCAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGt                             >  1:556130/1‑140 (MQ=255)

                                                                                                            cgcAATTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTc                          >  2:1166423/1‑140 (MQ=255)

                                                                                                                aTTGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACtt                      >  1:663703/1‑140 (MQ=255)

                                                                                                                 ttGTTAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTc                     <  2:654134/140‑1 (MQ=255)

                                                                                                                    ttAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGc                  <  1:723526/140‑1 (MQ=255)

                                                                                                                    ttAATGCTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGc                  <  1:806087/140‑1 (MQ=255)

                                                                                                                          cTGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTc            <  2:1037561/140‑1 (MQ=255)

                                                                                                                           tGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGttt                           <  2:604553/124‑1 (MQ=255)

                                                                                                                           tGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGttt                           >  1:604553/1‑124 (MQ=255)

                                                                                                                           tGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTCa           >  1:1052955/1‑140 (MQ=255)

                                                                                                                           tGGAGATATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTCa           >  2:962105/1‑140 (MQ=255)

                                                                                                                              agaTATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCa                 >  1:1028321/1‑131 (MQ=255)

                                                                                                                              agaTATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCa                 <  2:1028321/131‑1 (MQ=255)

                                                                                                                              agaTATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTCACGt        >  2:932316/1‑140 (MQ=255)

                                                                                                                               gaTATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTCACGTc       <  2:663703/140‑1 (MQ=255)

                                                                                                                               gaTATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTCACGTc       <  1:189536/140‑1 (MQ=255)

                                                                                                                               gaTATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTCACGTc       <  1:293923/140‑1 (MQ=255)

                                                                                                                               gaTATTGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTCACGTc       <  1:100824/140‑1 (MQ=255)

                                                                                                                                   ttGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTCACGTCCCgg   <  1:820879/140‑1 (MQ=255)

                                                                                                                                    tGCCAACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTCACGTCCCGGc  <  1:458471/140‑1 (MQ=255)

CCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTCACGTCCCGGC  >  NC_000913/3862088‑3862359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A