Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,999,973	T→C	V664A (GTC→GCC)	uvrD →	ssDNA translocase and dsDNA helicase ‑ DNA helicase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,999,973	0	T	C	100.0%	313.9 / NA	87	V664A (GTC→GCC)	uvrD	ssDNA translocase and dsDNA helicase ‑ DNA helicase II
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (41/46); total (41/46)

TATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGCCAGGGTATTAAATG  >  NC_000913/3999843‑4000108

tATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGa                                                                                                                                <  1:213576/140‑1 (MQ=255)

  tGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGATAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACa                                                                                                                              <  1:28410/140‑1 (MQ=255)

    gTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGc                                                                                                                            >  1:645422/1‑140 (MQ=255)

       aaGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGc                                                                                                                         >  1:351515/1‑140 (MQ=255)

       aaGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGc                                                                                                                         >  2:1088396/1‑140 (MQ=255)

        agagGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCt                                                                                                                        >  1:621506/1‑140 (MQ=255)

        agagGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAACCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCt                                                                                                                        >  2:962340/1‑140 (MQ=255)

          agGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTAc                                                                                                                      >  2:611664/1‑140 (MQ=255)

          agGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTAc                                                                                                                      >  1:494547/1‑140 (MQ=255)

          agGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTAc                                                                                                                      >  2:931636/1‑140 (MQ=255)

          agGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTAc                                                                                                                      >  1:18806/1‑140 (MQ=255)

            gTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACaa                                                                                                                    >  1:398626/1‑140 (MQ=255)

               tACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCt                                                                                                                 <  2:494547/140‑1 (MQ=255)

               tACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCt                                                                                                                 >  1:654637/1‑140 (MQ=255)

               tACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCt                                                                                                                 >  2:1060999/1‑140 (MQ=255)

               tACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGCAAGCCGCCCGGTCAGCCATCAGCGGATGAGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCt                                                                                                                 >  2:1091107/1‑140 (MQ=255)

                  cATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCgg                                                                                                              >  2:105470/1‑140 (MQ=255)

                    tCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGcc                                                                                                            <  2:18806/140‑1 (MQ=255)

                    tCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGcc                                                                                                            >  2:260582/1‑140 (MQ=255)

                    tCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGcc                                                                                                            <  1:566854/140‑1 (MQ=255)

                     cGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCa                                                                                                           <  2:654637/140‑1 (MQ=255)

                      gCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAg                                                                                                          >  2:795177/1‑140 (MQ=255)

                      gCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAg                                                                                                          >  2:1017658/1‑140 (MQ=255)

                           tCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGt                                                                                                     >  2:652153/1‑140 (MQ=255)

                               gcTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGc                                                                                                 <  1:213554/140‑1 (MQ=255)

                               gcTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGc                                                                                                 <  2:30059/140‑1 (MQ=255)

                                   tATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACg                                                                                             <  1:48201/140‑1 (MQ=255)

                                    aTCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGc                                                                                            <  1:611664/140‑1 (MQ=255)

                                     ttggCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCt                                                                                           <  1:909927/138‑1 (MQ=255)

                                           aGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGttt                                                                                     >  2:371507/1‑140 (MQ=255)

                                             cTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTgg                                                                                   >  2:201820/1‑140 (MQ=255)

                                             cTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTgg                                                                                   >  2:1235585/1‑140 (MQ=255)

                                                    aaGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGc                                                                            <  1:1017658/140‑1 (MQ=255)

                                                     agagTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCa                                                                           <  1:705423/140‑1 (MQ=255)

                                                     agagTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCa                                                                           <  2:398626/140‑1 (MQ=255)

                                                     agagTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCa                                                                           >  2:1005120/1‑140 (MQ=255)

                                                      gagTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCAc                                                                          >  1:1119525/1‑140 (MQ=255)

                                                         tgtgtgGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCAt                                                                       <  1:931636/140‑1 (MQ=255)

                                                          gtgtgGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCAcc                                                                         <  1:1014707/137‑1 (MQ=255)

                                                          gtgtgGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCAcc                                                                         >  2:1014707/1‑137 (MQ=255)

                                                          gtgtgGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCAtt                                                                      <  2:807683/140‑1 (MQ=255)

                                                          gtgtgGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCAtt                                                                      >  1:848056/1‑140 (MQ=255)

                                                            gtgGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGt                                                                    <  2:229787/140‑1 (MQ=255)

                                                            gtgGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGt                                                                    <  2:844052/140‑1 (MQ=255)

                                                            gtgGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGt                                                                    <  1:1251457/140‑1 (MQ=255)

                                                              ggAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCa                                                                  <  1:652153/140‑1 (MQ=255)

                                                                aaGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAgg                                                                             <  1:1230959/127‑1 (MQ=255)

                                                                aaGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAgg                                                                             >  2:1230959/1‑127 (MQ=255)

                                                                   agGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATg                                                             >  1:838176/1‑140 (MQ=255)

                                                                     gTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGa                                                           >  1:459817/1‑140 (MQ=255)

                                                                        cgcCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAgg                                                        >  1:491869/1‑140 (MQ=255)

                                                                        cgcCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAgg                                                        <  2:491869/140‑1 (MQ=255)

                                                                        cgcCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAgg                                                        <  1:1235585/140‑1 (MQ=255)

                                                                         gcCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGc                                                       >  2:429058/1‑140 (MQ=255)

                                                                             gcgcgcCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCg                                                   <  1:1060999/140‑1 (MQ=255)

                                                                               gcgcCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGt                                                 <  2:799782/140‑1 (MQ=255)

                                                                                   cACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGc                                             >  2:243812/1‑140 (MQ=255)

                                                                                    aCGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCa                                            <  2:645422/140‑1 (MQ=255)

                                                                                    aCGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCa                                            <  2:633666/140‑1 (MQ=255)

                                                                                     cGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCAt                                           >  1:1249644/1‑140 (MQ=255)

                                                                                       gTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAg                                         <  1:1088396/140‑1 (MQ=255)

                                                                                           gccgccCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGt                                     >  1:867174/1‑140 (MQ=255)

                                                                                              gccCGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTg                                  <  1:1091107/140‑1 (MQ=255)

                                                                                                 cGGTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAg                               <  2:351515/140‑1 (MQ=255)

                                                                                                  ggTCAGCCATCAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAgg                              >  1:190045/1‑140 (MQ=255)

                                                                                                            cAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCAttt                      >  2:359401/1‑138 (MQ=255)

                                                                                                            cAGCGGATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCAttt                      <  1:359401/138‑1 (MQ=255)

                                                                                                                ggATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGc                <  2:459817/140‑1 (MQ=255)

                                                                                                                 gATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGcc               <  2:867174/140‑1 (MQ=255)

                                                                                                                 gATGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGcc               <  2:848056/140‑1 (MQ=255)

                                                                                                                  aTGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGc                >  1:850085/1‑138 (MQ=255)

                                                                                                                  aTGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGc                <  2:850085/138‑1 (MQ=255)

                                                                                                                  aTGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGCCa              <  2:759502/140‑1 (MQ=255)

                                                                                                                  aTGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGCCa              <  1:873665/140‑1 (MQ=255)

                                                                                                                  aTGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGCCa              >  2:696076/1‑140 (MQ=255)

                                                                                                                  aTGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGCCa              <  1:795177/140‑1 (MQ=255)

                                                                                                                  aTGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGCCa              <  1:696076/140‑1 (MQ=255)

                                                                                                                  aTGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGCCa              <  1:423699/140‑1 (MQ=255)

                                                                                                                   tGGGTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGCGAg             <  2:621506/140‑1 (MQ=255)

                                                                                                                    gggTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGCCAgg            <  2:1249644/140‑1 (MQ=255)

                                                                                                                    gggTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGCCAgg            <  1:260582/140‑1 (MQ=255)

                                                                                                                      gTACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGCCAGGGt          <  2:1119525/140‑1 (MQ=255)

                                                                                                                       tACGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGCCAGGGTa         <  1:201820/140‑1 (MQ=255)

                                                                                                                         cGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGc                >  1:1193022/1‑131 (MQ=255)

                                                                                                                         cGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGc                <  2:1193022/131‑1 (MQ=255)

                                                                                                                         cGCCGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGCCAGGGTAtt       >  1:1216371/1‑140 (MQ=255)

                                                                                                                            cGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGTAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGc                <  2:213442/128‑1 (MQ=255)

                                                                                                                            cGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGc                >  1:213442/1‑128 (MQ=255)

                                                                                                                            cGATGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGCCAGGGTATTaaa    >  1:214746/1‑140 (MQ=255)

                                                                                                                              aTGGCCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGCCAGGGTATTAAATg  >  1:1177697/1‑140 (MQ=255)

TATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAGTTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTGGCATTTCAGGGCCAGGGTATTAAATG  >  NC_000913/3999843‑4000108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A