Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,030,743	A→G	F77L (TTC→CTC)	fadB ←	dodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,030,743	0	A	G	100.0%	182.0 / NA	52	F77L (TTC→CTC)	fadB	dodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (32/20); total (32/20)

CACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTC  >  NC_000913/4030609‑4030878

cACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAggg                                                                                                                                    >  1:238879/1‑140 (MQ=255)

     aTTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACaaa                                                                                                                               >  1:1190656/1‑140 (MQ=255)

     aTTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACaaa                                                                                                                               >  2:715698/1‑140 (MQ=255)

       tCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACaaaaa                                                                                                                             <  2:958754/140‑1 (MQ=255)

        cGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAAt                                                                                                                            <  1:1230699/140‑1 (MQ=255)

          cAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTc                                                                                                                          >  1:1186990/1‑140 (MQ=255)

           aGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCg                                                                                                                         <  1:300432/140‑1 (MQ=255)

             ccACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGt                                                                                                                       <  2:141861/140‑1 (MQ=255)

             ccACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGt                                                                                                                       <  2:664407/140‑1 (MQ=255)

             ccACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGt                                                                                                                       <  1:468593/140‑1 (MQ=255)

                          cATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACcg                                                                                                          <  2:210682/140‑1 (MQ=255)

                           aTAGCCATTGACGGCAGCAATGGTCAGCACCGGCAGATCTTCCAGGCGATTAAACACGCCATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACcga                                                                                                         >  2:599297/1‑140 (MQ=255)

                               ccATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGAt                                                                                                     <  2:1099938/140‑1 (MQ=255)

                                  ttGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATaaa                                                                                                  >  2:338149/1‑140 (MQ=255)

                                         cagcaATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGgctgct                                                                                           >  2:381738/1‑140 (MQ=255)

                                            caATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTg                                                                                        >  1:53697/1‑140 (MQ=255)

                                                    ggCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACg                                                                                <  1:468845/140‑1 (MQ=255)

                                                       aCCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACgcag                                                                             >  2:1201418/1‑140 (MQ=255)

                                                              gATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCagcagcagc                                                                      <  1:601016/140‑1 (MQ=255)

                                                              gATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCagcagcagc                                                                      <  2:1186990/140‑1 (MQ=255)

                                                               aTCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGcc                                                                     >  2:89334/1‑140 (MQ=255)

                                                                      aGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAg                                                              >  1:647842/1‑140 (MQ=255)

                                                                           aTTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCagcagcagc                                                                      >  2:799424/1‑127 (MQ=255)

                                                                           aTTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCagcagcagc                                                                      <  1:799424/127‑1 (MQ=255)

                                                                           aTTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTg                                                         >  1:1136706/1‑140 (MQ=255)

                                                                            ttAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGa                                                        >  2:842408/1‑140 (MQ=255)

                                                                             tAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGAt                                                       <  1:992332/140‑1 (MQ=255)

                                                                               aaCACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTg                                                     <  1:715698/140‑1 (MQ=255)

                                                                                      tATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTcc                                              >  2:128814/1‑140 (MQ=255)

                                                                                                gTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGa                                    <  2:1136706/140‑1 (MQ=255)

                                                                                                  gCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATg                                  >  2:285513/1‑140 (MQ=255)

                                                                                                           gACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGcc                          <  2:63778/139‑1 (MQ=255)

                                                                                                           gACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGcc                          >  1:63778/1‑139 (MQ=255)

                                                                                                           gACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCg                         >  1:308448/1‑140 (MQ=255)

                                                                                                                    gTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCg                         >  1:658242/1‑131 (MQ=255)

                                                                                                                    gTTCTTCAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCg                         <  2:658242/131‑1 (MQ=255)

                                                                                                                      tcttcAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAgg                      <  2:1243363/132‑1 (MQ=255)

                                                                                                                      tcttcAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAgg                      >  1:1243363/1‑132 (MQ=255)

                                                                                                                      tcttcAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGAc              >  1:109679/1‑140 (MQ=255)

                                                                                                                      tcttcAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGAc              >  2:772855/1‑140 (MQ=255)

                                                                                                                      tcttcAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGAc              >  1:594824/1‑140 (MQ=255)

                                                                                                                       cttcAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACg             >  2:735917/1‑140 (MQ=255)

                                                                                                                        ttcAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACgg            >  1:751960/1‑140 (MQ=255)

                                                                                                                        ttcAGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACgg            >  1:211582/1‑140 (MQ=255)

                                                                                                                           aGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCg         <  2:308448/140‑1 (MQ=255)

                                                                                                                           aGGAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCg         <  1:89334/140‑1 (MQ=255)

                                                                                                                             gAACGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCa       >  1:80208/1‑140 (MQ=255)

                                                                                                                                cGAGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAgtg    >  1:342293/1‑140 (MQ=255)

                                                                                                                                 gaggagCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAgtgt   <  2:109679/140‑1 (MQ=255)

                                                                                                                                  aGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTc  >  2:711703/1‑140 (MQ=255)

                                                                                                                                  aGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTc  >  2:54289/1‑140 (MQ=255)

                                                                                                                                  aGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTc  >  2:920017/1‑140 (MQ=255)

                                                                                                                                  aGGAGCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTc  >  1:650719/1‑140 (MQ=255)

CACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTC  >  NC_000913/4030609‑4030878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A