Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 260 73.5 2514320 99.3% 2496719 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,250,294 A→G A247A (GCA→GCG)  malM → maltose regulon periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,250,2940AG100.0% 276.4 / NA 77A247A (GCA→GCG) malMmaltose regulon periplasmic protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/35);  total (42/35)

GATGGCTTACTGAAACTGAAAGTGAAAACGAACTCCAGCTCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCACCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTTGATGAAGCTGAACGCCTGGGATCGA  >  NC_000913/4250157‑4250427
                                                                                                                                         |                                                                                                                                     
gATGGCTTACTGAAACTGAAAGTGAAAACGAACTCCAGCTCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGcc                                                                                                                                     >  1:661790/1‑140 (MQ=255)
 aTGGCTTACTGAAACTGAAAGTGAAAACGAACTCCAGCTCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCg                                                                                                                                    >  2:254758/1‑140 (MQ=255)
 aTGGCTTACTGAAACTGAAAGTGAAAACGAACTCCAGCTCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCg                                                                                                                                    >  2:1159147/1‑140 (MQ=255)
 aTGGCTTACTGAAACTGAAAGTGAAAACGAACTCCAGCTCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCg                                                                                                                                    >  1:939283/1‑140 (MQ=255)
 aTGGCTTACTGAAACTGAAAGTGAAAACGAACTCCAGCTCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCg                                                                                                                                    >  2:844824/1‑140 (MQ=255)
 aTGGCTTACTGAAACTGAAAGTGAAAACGAACTCCAGCTCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCg                                                                                                                                    >  2:856088/1‑140 (MQ=255)
 aTGGCTTACTGAAACTGAAAGTGAAAACGAACTCCAGCTCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGATAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCg                                                                                                                                    >  2:1164946/1‑140 (MQ=255)
   ggCTTACTGAAACTGAAAGTGAAAACGAACTCCAGCTCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGt                                                                                                                                  <  2:219402/140‑1 (MQ=255)
              actgaaaGTGAAAACGAACTCCAGCTCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCg                                                                                                                       <  2:992016/140‑1 (MQ=255)
                 gaaaGTGAAAACGAACTCCAGCTCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGc                                                                                                                    <  1:1009743/140‑1 (MQ=255)
                   aaGTGAAAACGAACTCCAGCCCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGAGCCGGTGAAGAAAAGCGAGCCg                                                                                                                  <  2:755722/140‑1 (MQ=255)
                    aGTGAAAACGAACTCCAGCTCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGa                                                                                                                 <  2:213314/140‑1 (MQ=255)
                    aGTGAAAACGAACTCCAGCTCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGa                                                                                                                 >  1:477102/1‑140 (MQ=255)
                    aGTGAAAACGAACTCCAGCTCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGa                                                                                                                 >  1:416204/1‑140 (MQ=255)
                      tGAAAACGAACTCCAGCTCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATg                                                                                                               <  2:407234/140‑1 (MQ=255)
                        aaaaCGAACTCCAGCTCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCt                                                                                                             >  2:311371/1‑140 (MQ=255)
                          aaCGAACTCCAGCTCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCa                                                                                                           >  1:350202/1‑140 (MQ=255)
                                  ccagctccagcGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACg                                                                                                   <  1:1244205/140‑1 (MQ=255)
                                    agctccagcGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGa                                                                                                 <  1:433264/140‑1 (MQ=255)
                                        ccagcGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGt                                                                                             <  1:423758/140‑1 (MQ=255)
                                        ccagcGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGt                                                                                             <  2:416204/140‑1 (MQ=255)
                                        ccagcGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGt                                                                                             <  1:1159147/140‑1 (MQ=255)
                                        ccagcGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGt                                                                                             <  1:844824/140‑1 (MQ=255)
                                        ccagcGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGt                                                                                             <  2:687351/140‑1 (MQ=255)
                                          agcGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTa                                                                                           <  1:145034/140‑1 (MQ=255)
                                           gcGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTAt                                                                                          >  2:813217/1‑140 (MQ=255)
                                                   gTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATAc                                                                                  >  1:33770/1‑140 (MQ=255)
                                                     aGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACcg                                                                                <  1:1141208/140‑1 (MQ=255)
                                                       gACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACcgcg                                                                              <  2:350202/140‑1 (MQ=255)
                                                        aCCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGa                                                                             >  2:1044420/1‑140 (MQ=255)
                                                        aCCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGGAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGa                                                                             >  2:837107/1‑140 (MQ=255)
                                                          cTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATc                                                                           >  1:413691/1‑140 (MQ=255)
                                                             tATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCaaa                                                                        >  1:1107237/1‑140 (MQ=255)
                                                                 tGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACg                                                                    <  1:254758/140‑1 (MQ=255)
                                                                   gTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCt                                                                  <  2:915774/140‑1 (MQ=255)
                                                                    ttcttcCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTg                                                                 >  2:810479/1‑140 (MQ=255)
                                                                    ttcttcCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAAAGCTg                                                                 >  2:735446/1‑140 (MQ=255)
                                                                    ttcttcCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACAAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTg                                                                 >  2:313111/1‑140 (MQ=255)
                                                                       ttcCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTcg                                                              >  2:116675/1‑140 (MQ=255)
                                                                             tCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAg                                                        <  2:1107237/140‑1 (MQ=255)
                                                                             tCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAg                                                        >  2:130151/1‑140 (MQ=255)
                                                                               cAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGt                                                      <  2:33770/140‑1 (MQ=255)
                                                                               cAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGt                                                      <  1:53891/140‑1 (MQ=255)
                                                                                aGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTg                                                     <  1:699105/140‑1 (MQ=255)
                                                                                       gTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGa                                              >  1:689440/1‑140 (MQ=255)
                                                                                          aCGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATaa                                           <  1:422874/140‑1 (MQ=255)
                                                                                                 gtaACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAATACGCTGTCGCGAAAGGCGATGTTGATAAGGCGTTa                                    >  2:871778/1‑140 (MQ=255)
                                                                                                  taACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTaa                                   <  1:1164946/140‑1 (MQ=255)
                                                                                                     cacGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAAc                                >  2:473897/1‑140 (MQ=255)
                                                                                                     cacGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAAc                                >  2:292878/1‑140 (MQ=255)
                                                                                                     cacGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAAc                                >  2:507853/1‑140 (MQ=255)
                                                                                                      acGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACt                               >  2:427272/1‑140 (MQ=255)
                                                                                                       cggcggCACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTg                              <  1:311371/140‑1 (MQ=255)
                                                                                                        ggcggcACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTaaaa                                 <  2:464748/136‑1 (MQ=255)
                                                                                                        ggcggcACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTaaaa                                 >  1:464748/1‑136 (MQ=255)
                                                                                                        ggcggcACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGc                             >  2:1065020/1‑140 (MQ=255)
                                                                                                          cggcACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATaa                                           <  1:1059713/124‑1 (MQ=255)
                                                                                                          cggcACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATaa                                           >  2:1059713/1‑124 (MQ=255)
                                                                                                          cggcACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCtt                           >  2:162823/1‑140 (MQ=255)
                                                                                                          cggcACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCtt                           >  1:1091360/1‑140 (MQ=255)
                                                                                                          cggcACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCtt                           >  1:728607/1‑140 (MQ=255)
                                                                                                            gcACCAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTtga                         >  1:204917/1‑140 (MQ=255)
                                                                                                              cccAGCTGTGGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTtgatg                       <  2:661790/139‑1 (MQ=255)
                                                                                                                     gtgGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTTGATGAAGCTGa                <  1:813217/140‑1 (MQ=255)
                                                                                                                     gtgGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTTGATGAAGCTGa                <  1:374589/140‑1 (MQ=255)
                                                                                                                      tgGCTGCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTTGATGAAGCTGaa               >  2:984051/1‑140 (MQ=255)
                                                                                                                           gCACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTTGATGAAGCTGAACGCCt          >  2:706465/1‑140 (MQ=255)
                                                                                                                            cACCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTTGATGAAGCTGAACGCCTg         <  1:427272/140‑1 (MQ=255)
                                                                                                                             aCCCGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTTGATGAAGCTGAACGCCTgg        >  1:115780/1‑140 (MQ=255)
                                                                                                                                cGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTTGATGAAGCTGAACGCCTGGGAt     <  2:1091360/140‑1 (MQ=255)
                                                                                                                                cGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTTGATGAAGCTGAACGCCTGGGAt     >  2:44654/1‑140 (MQ=255)
                                                                                                                                cGCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTTGATGAAGCTGAACGCCTGGGAt     <  1:706465/140‑1 (MQ=255)
                                                                                                                                 gCTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTTGATGAAGCTGAACGCCTGGGATc    >  2:641189/1‑140 (MQ=255)
                                                                                                                                  cTCCGGCGCCGGTGAAGGAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTTGATGAAGCTGAACGCCTGGGATCg   >  2:113333/1‑140 (MQ=255)
                                                                                                                                  cTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTTGATGAAGCTGAACGCCTGGGAt     <  1:372869/138‑1 (MQ=255)
                                                                                                                                  cTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTTGATGAAGCTGAACGCCTGGGAt     >  2:372869/1‑138 (MQ=255)
                                                                                                                                  cTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTTGATGAAGCTGAACGCCTGGGATCg   >  1:206918/1‑140 (MQ=255)
                                                                                                                                  cTCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTTGATGAAGCTGAACGCCTGGGATCg   >  1:1061200/1‑140 (MQ=255)
                                                                                                                                   tCCGGCGCCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTTGATGAAGCTGAACGCCTGGGATCGa  <  2:685368/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                     
GATGGCTTACTGAAACTGAAAGTGAAAACGAACTCCAGCTCCAGCGTGTTGGTAGGACCTTTATTTGGTTCTTCCGCTCCAGCTCCGGTTACGGTAGGTAACACGGCGGCACCAGCTGTGGCTGCACCCGCTCCGGCACCGGTGAAGAAAAGCGAGCCGATGCTCAACGACACGGAAAGTTATTTTAATACCGCGATCAAAAACGCTGTCGCGAAAGGTGATGTTGATAAGGCGTTAAAACTGCTTGATGAAGCTGAACGCCTGGGATCGA  >  NC_000913/4250157‑4250427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A