Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,321,237	T→C	E4G (GAA→GGA)	phnH ←	carbon‑phosphorus lyase core complex subunit PhnH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,321,237	0	T	C	100.0%	215.2 / NA	60	E4G (GAA→GGA)	phnH	carbon‑phosphorus lyase core complex subunit PhnH
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (34/26); total (34/26)

TGGTGGTGGCGATATTCAGCGGTTGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGA  >  NC_000913/4321100‑4321373

tggtggtggCGATATTCAGCGGTTGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTccc                                                                                                                                        <  1:1083717/140‑1 (MQ=255)

  gtggtggCGATATTCAGCGGTTGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAg                                                                                                                                      >  1:637504/1‑140 (MQ=255)

  gtggtggCGATATTCAGCGGTTGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAg                                                                                                                                      >  1:346518/1‑140 (MQ=255)

     gtggCGATATTCAGCGGTTGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGTCGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGt                                                                                                                                   <  2:544781/140‑1 (MQ=255)

     gtggCGATATTCAGCGGTTGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGt                                                                                                                                   <  2:7048/140‑1 (MQ=255)

       ggCGATATTCAGCGGTTGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCa                                                                                                                                 >  2:294434/1‑140 (MQ=255)

            tatTCAGCGGTTGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGt                                                                                                                            >  1:630998/1‑140 (MQ=255)

                  gCGGTTGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGtctc                                                                                                                      >  1:443171/1‑140 (MQ=255)

                   cGGTTGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGt                                                                                                                                   <  2:659486/126‑1 (MQ=255)

                   cGGTTGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGt                                                                                                                                   >  1:659486/1‑126 (MQ=255)

                   cGGTTGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTcc                                                                                                                     >  2:188745/1‑140 (MQ=255)

                   cGGTTGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTcc                                                                                                                     <  1:188745/140‑1 (MQ=255)

                      ttGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCcgcg                                                                                                                  >  2:424879/1‑140 (MQ=255)

                        gCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGaa                                                                                                                >  1:277701/1‑140 (MQ=255)

                        gCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGaa                                                                                                                <  2:637504/140‑1 (MQ=255)

                        gCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGaa                                                                                                                <  2:1021773/140‑1 (MQ=255)

                         ccAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAAc                                                                                                               <  2:277701/140‑1 (MQ=255)

                         ccAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAAc                                                                                                               >  1:1146550/1‑140 (MQ=255)

                                cgTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTa                                                                                                        >  1:479943/1‑140 (MQ=255)

                                  tttGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAAc                                                                                                               >  1:933454/1‑131 (MQ=255)

                                  tttGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAAc                                                                                                               <  2:933454/131‑1 (MQ=255)

                                           aTGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGAc                                                                                             <  2:462463/140‑1 (MQ=255)

                                           aTGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGAc                                                                                             <  2:696073/140‑1 (MQ=255)

                                           aTGCAGGGCGACAATCACGCACGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGAc                                                                                             >  1:236116/1‑140 (MQ=255)

                                                  gCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTg                                                                                      <  1:598199/140‑1 (MQ=255)

                                                     aCAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCg                                                                                   >  1:31899/1‑140 (MQ=255)

                                                      cAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGt                                                                                  >  1:828057/1‑140 (MQ=255)

                                                       aaTCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGtt                                                                                 <  2:479943/140‑1 (MQ=255)

                                                       aaTCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGtt                                                                                 <  2:1236851/140‑1 (MQ=255)

                                                           acacccGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACt                                                                             <  2:368471/137‑1 (MQ=255)

                                                                cGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGc                                                                        >  2:29778/1‑140 (MQ=255)

                                                                 ggCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGcc                                                                       <  2:1146550/140‑1 (MQ=255)

                                                                   ctcgctcATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTg                                                                     >  1:475511/1‑140 (MQ=255)

                                                                     cgctcATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGc                                                                   <  2:54457/140‑1 (MQ=255)

                                                                       ctcATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGt                                                                 >  2:613228/1‑140 (MQ=255)

                                                                       ctcATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGt                                                                 >  1:543715/1‑140 (MQ=255)

                                                                       ctcATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGt                                                                 >  2:8922/1‑140 (MQ=255)

                                                                          aTGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAG‑CGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCgg                                                             <  2:443171/140‑1 (MQ=255)

                                                                           tGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCgg                                                             >  2:330754/1‑140 (MQ=255)

                                                                              ccTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCaa                                                          <  2:346518/140‑1 (MQ=255)

                                                                               cTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAAt                                                         <  1:704255/140‑1 (MQ=255)

                                                                                  tAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATTCg                                                      >  2:707710/1‑140 (MQ=255)

                                                                                  tAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCg                                                      >  2:350512/1‑140 (MQ=255)

                                                                                   aaCAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGt                                                     <  1:424879/140‑1 (MQ=255)

                                                                                     cAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGc                                                   <  2:475511/140‑1 (MQ=255)

                                                                                      aGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGcc                                                  >  2:890474/1‑140 (MQ=255)

                                                                                        gCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCAt                                                >  1:975420/1‑140 (MQ=255)

                                                                                                       cTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAg                                 <  2:630998/140‑1 (MQ=255)

                                                                                                       cTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAg                                 <  1:890474/140‑1 (MQ=255)

                                                                                                          ggCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCgg                              >  2:1001877/1‑140 (MQ=255)

                                                                                                          ggCATCCTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCgg                              >  2:96889/1‑140 (MQ=255)

                                                                                                                cTGCACGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAAt                        <  1:8922/140‑1 (MQ=255)

                                                                                                                    aCGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATa                       >  2:796528/1‑137 (MQ=255)

                                                                                                                    aCGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATa                       <  1:796528/137‑1 (MQ=255)

                                                                                                                    aCGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAgg                    >  1:819178/1‑140 (MQ=255)

                                                                                                                     cGGGAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAggg                   <  2:31899/140‑1 (MQ=255)

                                                                                                                        gAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGttt                >  1:1155940/1‑140 (MQ=255)

                                                                                                                        gAAGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGttt                >  2:1142858/1‑140 (MQ=255)

                                                                                                                         aaGCATAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTc               >  2:824647/1‑140 (MQ=255)

                                                                                                                             aTAAAAGCGGTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGAt           >  1:171042/1‑140 (MQ=255)

                                                                                                                                      gTTCCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGa  >  2:383089/1‑140 (MQ=255)

TGGTGGTGGCGATATTCAGCGGTTGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGA  >  NC_000913/4321100‑4321373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A