Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,546,391	G→A	G432G (GGG→GGA)	fimD →	type I fimbriae usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,546,391	0	G	A	99.2%	444.7 / ‑5.1	126	G432G (GGG→GGA)	fimD	type I fimbriae usher protein
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (60/65); total (61/65)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 130 total aligned reads displayed.

ATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGG  >  NC_000913/4546255‑4546527

aTGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCa                                                                                                                                       >  2:888790/1‑140 (MQ=255)

aTGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCa                                                                                                                                       >  1:1168604/1‑140 (MQ=255)

aTGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCa                                                                                                                                       >  2:149624/1‑140 (MQ=255)

         agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGct                                                                                                                              <  2:91010/140‑1 (MQ=255)

         agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGct                                                                                                                              <  2:1071964/140‑1 (MQ=255)

         agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGct                                                                                                                              <  2:1106239/140‑1 (MQ=255)

         agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGct                                                                                                                              <  2:1162290/140‑1 (MQ=255)

         agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGct                                                                                                                              <  2:1168604/140‑1 (MQ=255)

         agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGct                                                                                                                              <  1:683526/140‑1 (MQ=255)

         agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGct                                                                                                                              <  1:477856/140‑1 (MQ=255)

         agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGct                                                                                                                              <  2:264859/140‑1 (MQ=255)

         agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGct                                                                                                                              <  2:266818/140‑1 (MQ=255)

         agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGct                                                                                                                              <  1:428636/140‑1 (MQ=255)

         agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGct                                                                                                                              <  2:537511/140‑1 (MQ=255)

         agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGct                                                                                                                              <  1:142351/140‑1 (MQ=255)

         agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGct                                                                                                                              <  2:757490/140‑1 (MQ=255)

         agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGct                                                                                                                              <  1:1198695/140‑1 (MQ=255)

          ggAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGctc                                                                                                                             <  2:1164223/140‑1 (MQ=255)

           gAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGctct                                                                                                                            <  2:71566/140‑1 (MQ=255)

           gAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGctct                                                                                                                            <  2:711920/140‑1 (MQ=255)

            aaaaaaCCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCtctg                                                                                                                           <  1:321601/140‑1 (MQ=255)

             aaaaaCCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCtctgt                                                                                                                          <  1:613175/140‑1 (MQ=255)

                 aCCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCATCTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTctgtctgt                                                                                                                      <  2:872969/140‑1 (MQ=255)

                 aCCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTctgtctgt                                                                                                                      <  1:961569/140‑1 (MQ=255)

                  cccGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGct                                                                                                                              >  2:642713/1‑131 (MQ=255)

                  cccGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGct                                                                                                                              <  1:642713/131‑1 (MQ=255)

                  cccGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCtgtg                                                                                                                     >  1:339599/1‑140 (MQ=255)

                    cGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTgg                                                                                                                                   <  2:1243978/124‑1 (MQ=255)

                    cGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTgg                                                                                                                                   >  1:1243978/1‑124 (MQ=255)

                                agTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGc                                                                                                       <  2:1116512/140‑1 (MQ=255)

                                 gTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCt                                                                                                      <  1:149624/140‑1 (MQ=255)

                                     aTTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGa                                                                                                              <  1:753761/128‑1 (MQ=255)

                                     aTTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTCGGCGCTCTGTCTGTGGATATGACGCAGGCTAAtt                                                                                                  >  1:929848/1‑140 (MQ=255)

                                          tCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCcaca                                                                                             >  1:667811/1‑140 (MQ=255)

                                           ccACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCacac                                                                                            <  2:339599/140‑1 (MQ=255)

                                           ccACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCacac                                                                                            <  1:354313/140‑1 (MQ=255)

                                            cACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACt                                                                                           >  1:809755/1‑140 (MQ=255)

                                              cGGCCTTCCGGCTGGCTGGACAATATATGGTGGCACGCAACTCGCGGGTCGTTCTCGTGCTTTTACTTTCGGTATCGGGTAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGGAGGCTAATTCCACACTTc                                                                                         >  2:1076848/1‑140 (MQ=255)

                                              cGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGc                                                                                                       >  1:1251634/1‑126 (MQ=255)

                                              cGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTc                                                                                         >  2:844743/1‑140 (MQ=255)

                                              cGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTc                                                                                         >  1:280611/1‑140 (MQ=255)

                                               ggCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGc                                                                                                       <  2:855499/125‑1 (MQ=255)

                                               ggCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGc                                                                                                       >  1:855499/1‑125 (MQ=255)

                                               ggCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTcc                                                                                        >  2:778848/1‑140 (MQ=255)

                                                 ccTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCg                                                                                      >  2:368242/1‑140 (MQ=255)

                                                 ccTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCg                                                                                      >  1:407559/1‑140 (MQ=255)

                                                   ttCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGAt                                                                                    >  2:417172/1‑140 (MQ=255)

                                                    tCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATg                                                                                   <  2:39541/140‑1 (MQ=255)

                                                        gctggctgGAC‑ATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGt                                                                              <  2:1226842/140‑1 (MQ=255)

                                                           ggctggACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCa                                                                            >  2:944285/1‑140 (MQ=255)

                                                           ggctggACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCa                                                                            >  2:38501/1‑140 (MQ=255)

                                                           ggctggACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCa                                                                            >  2:816689/1‑140 (MQ=255)

                                                                          ggtggAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATc                                                             >  1:649201/1‑140 (MQ=255)

                                                                            tggAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTTCCGATGACAGTCAGCATGACGGACAATCgg                                                           >  1:1007721/1‑140 (MQ=255)

                                                                               aaCGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCa                                                                         <  1:1235250/123‑1 (MQ=255)

                                                                               aaCGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCa                                                                         >  2:1235250/1‑123 (MQ=255)

                                                                               aaCGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGc                                                        >  1:1187807/1‑140 (MQ=255)

                                                                               aaCGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGc                                                        >  1:280531/1‑140 (MQ=255)

                                                                               aaCGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGc                                                        >  2:1042962/1‑140 (MQ=255)

                                                                               aaCGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGc                                                        >  2:512558/1‑140 (MQ=255)

                                                                               aaCGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGc                                                        >  1:191743/1‑140 (MQ=255)

                                                                               aaCGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGc                                                        >  2:68965/1‑140 (MQ=255)

                                                                               aaCGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGc                                                        >  1:1165530/1‑140 (MQ=255)

                                                                               aaCGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGc                                                        >  2:879075/1‑140 (MQ=255)

                                                                               aaCGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGc                                                        >  1:770146/1‑140 (MQ=255)

                                                                               aaCGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGc                                                        >  1:1080515/1‑140 (MQ=255)

                                                                               aaCGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGc                                                        >  1:923935/1‑140 (MQ=255)

                                                                                  gCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCTCACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGtt                                                     <  1:1173755/140‑1 (MQ=255)

                                                                                  gCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGtt                                                     <  2:923935/140‑1 (MQ=255)

                                                                                   cAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGttt                                                    >  2:129415/1‑140 (MQ=255)

                                                                                    aaCTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGtttt                                                   <  1:477199/140‑1 (MQ=255)

                                                                                    aaCTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGtttt                                                   <  1:16607/140‑1 (MQ=255)

                                                                                      cTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTtct                                                 <  1:246782/140‑1 (MQ=255)

                                                                                        ggCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTctct                                               >  1:47279/1‑140 (MQ=255)

                                                                                              tCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACa                                         >  1:846910/1‑140 (MQ=255)

                                                                                                   aTCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCg                                    <  1:401624/140‑1 (MQ=255)

                                                                                                   aTCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCg                                    <  1:1247222/140‑1 (MQ=255)

                                                                                                      gTGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTc                                 <  1:512558/140‑1 (MQ=255)

                                                                                                       tGCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCa                                <  2:667811/140‑1 (MQ=255)

                                                                                                        gCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCaa                               <  2:407559/140‑1 (MQ=255)

                                                                                                        gCTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCaa                               >  1:323368/1‑140 (MQ=255)

                                                                                                         cTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCg                                    >  1:1255002/1‑134 (MQ=255)

                                                                                                         cTTTTAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCg                                    <  2:1255002/134‑1 (MQ=255)

                                                                                                           tttAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGa                            >  1:73644/1‑140 (MQ=255)

                                                                                                            ttAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGaa                           >  2:1250675/1‑140 (MQ=255)

                                                                                                             tAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAAt                          <  1:711763/140‑1 (MQ=255)

                                                                                                             tAATTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAAt                          <  2:1099950/140‑1 (MQ=255)

                                                                                                              aaTTTCGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATc                         >  2:365514/1‑140 (MQ=255)

                                                                                                                   cGGTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCa                    <  1:38501/140‑1 (MQ=255)

                                                                                                                     gTATCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGCCGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTAAATGAATCAGGCACg                  <  2:809755/140‑1 (MQ=255)

                                                                                                                        tCGGGAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAat               >  1:1088072/1‑140 (MQ=255)

                                                                                                                          gggAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAatat             >  2:489077/1‑140 (MQ=255)

                                                                                                                           ggAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAgg                      <  2:724851/130‑1 (MQ=255)

                                                                                                                           ggAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAgg                      >  1:724851/1‑130 (MQ=255)

                                                                                                                           ggAAAAACATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATAtt            >  1:108480/1‑140 (MQ=255)

                                                                                                                               aaaCATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGt        <  1:944285/140‑1 (MQ=255)

                                                                                                                                 aCATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTa      <  2:929848/140‑1 (MQ=255)

                                                                                                                                  cATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAg     <  1:152790/140‑1 (MQ=255)

                                                                                                                                  cATGGGAGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAg     <  2:280531/140‑1 (MQ=255)

                                                                                                                                     gggagCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTgg  >  2:629147/1‑140 (MQ=255)

ATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGG  >  NC_000913/4546255‑4546527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A