Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,589,124	C→T	intergenic (‑45/+5)	yjiX ← / ← btsT	conserved protein YjiX/pyruvate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,589,124	0	C	T	100.0%	187.8 / NA	54	intergenic (‑45/+5)	yjiX/btsT	conserved protein YjiX/pyruvate:H(+) symporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (24/30); total (24/30)

CTCGACGTAGTTGTCGTAGTCTGGAATGCCAATCAACATCTTCGCCGCCTGGCCGAGATATTTTTTTGCCTGTCCTAAGTTACCAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAACACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGAAACCGTAGAAGATGATGCTGTACACCA  >  NC_000913/4588990‑4589259

cTCGACGTAGTTGTCGTAGTCTGTAATGCCAATCAACATCTTCGCCGCCTGGCCGAGATATTTTTTTGCCTGTCCTAAGTTACCAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAatac                                                                                                                                       >  1:126727/1‑137 (MQ=255)

cTCGACGTAGTTGTCGTAGTCTGGAATGCCAATCAACATCTTCGCCGCCTGGCCGAGATATTTTTTTGCCTGTCCTAAGTTACCAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGt                                                                                                                                    <  2:215400/140‑1 (MQ=255)

   gACGTAGTTGTCGTAGTCTGGAATGCCAATCAACATCTTCGCCGCCTGGCCGAGATATTTTTTTGCCTGTCCTAAGTTACCAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAg                                                                                                                                 <  2:614977/140‑1 (MQ=255)

                       gAATGCCAATCAACATCTTCGCCGCCTGGCCGAGATATTTTTTTGCCTGTCCTAAGTTACCAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGaa                                                                                                                        <  2:1244083/129‑1 (MQ=255)

                       gAATGCCAATCAACATCTTCGCCGCCTGGCCGAGATATTTTTTTGCCTGTCCTAAGTTACCAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGaa                                                                                                                        >  1:1244083/1‑129 (MQ=255)

                        aaTGCCAATCAACATCTTCGCCGCCTGGCCGAGATATTTTTTTGCCTGTCCTAAGTTACCAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCAcg                                                                                                            >  2:809150/1‑140 (MQ=255)

                           gCCAATCAACATCTTCGCCGCCTGGCCGAGATATTTTTTTGCCTGTCCTAAGTTACCAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCAcgccg                                                                                                         <  1:482289/140‑1 (MQ=255)

                            ccAATCAACATCTTCGCCGCCTGGCCGAGATATTTTTTTGCCTGTCCTAAGTTACCAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCAcgccgc                                                                                                        >  2:1064611/1‑140 (MQ=255)

                                tCAACATCTTCGCCGCCTGGCCGAGATATTTTTTTGCCTGTCCTAAGTTACCAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTc                                                                                                    <  2:296944/140‑1 (MQ=255)

                                          cgccgcCTGGCCGAGATATTTTTTTGCCTGTCCTAAGTTACCAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGa                                                                                          >  2:832287/1‑140 (MQ=255)

                                                 tGGCCGAGATATTTTTTTGCCTGTCCTAAGTTACCAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACg                                                                                   <  2:826383/140‑1 (MQ=255)

                                                         atatTTTTTTGCCTGTCCTAAGTTACCAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCt                                                                           >  2:988811/1‑140 (MQ=255)

                                                          tatTTTTTTGCCTGTCCTAAGTTACCAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCtt                                                                          <  2:648648/140‑1 (MQ=255)

                                                           atTTTTTTGCCTGTCCTAAGTTACCAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCttt                                                                         <  1:988811/140‑1 (MQ=255)

                                                           atTTTTTTGCCTGTCCTAAGTTACCAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCttt                                                                         <  2:638080/140‑1 (MQ=255)

                                                                tttGCCTGTCCTAAGTTACCAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAg                                                                    <  1:1033240/140‑1 (MQ=255)

                                                                                  ccAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCATGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTAc                                                  >  2:832400/1‑140 (MQ=255)

                                                                                  ccAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCg                                                         >  1:712851/1‑133 (MQ=255)

                                                                                  ccAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCg                                                         <  2:712851/133‑1 (MQ=255)

                                                                                  ccAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTAc                                                  >  1:49991/1‑140 (MQ=255)

                                                                                  ccAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTAc                                                  >  1:95978/1‑140 (MQ=255)

                                                                                  ccAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTAc                                                  >  1:96031/1‑140 (MQ=255)

                                                                                  ccAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTAc                                                  >  1:216238/1‑140 (MQ=255)

                                                                                  ccAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTAc                                                  >  1:1075485/1‑140 (MQ=255)

                                                                                  ccAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTAc                                                  >  2:146316/1‑140 (MQ=255)

                                                                                            ttCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAg                                        >  1:607325/1‑140 (MQ=255)

                                                                                            ttCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAg                                        >  2:644402/1‑140 (MQ=255)

                                                                                               aCTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCa                                     <  1:832287/140‑1 (MQ=255)

                                                                                                ctctGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCAcc                                             >  1:1224395/1‑131 (MQ=255)

                                                                                                ctctGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCAcc                                             <  2:1224395/131‑1 (MQ=255)

                                                                                                 tctGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAgg                                   >  2:249022/1‑140 (MQ=255)

                                                                                                    gATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGttt                                >  2:284407/1‑140 (MQ=255)

                                                                                                    gATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGttt                                >  2:492408/1‑140 (MQ=255)

                                                                                                     aTAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGtttt                               >  2:408000/1‑140 (MQ=255)

                                                                                                      tAAGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTg                              <  2:607325/140‑1 (MQ=255)

                                                                                                       aaGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGa                             <  1:492408/140‑1 (MQ=255)

                                                                                                       aaGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGa                             <  2:95978/140‑1 (MQ=255)

                                                                                                       aaGAACAAAGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGa                             <  1:146316/140‑1 (MQ=255)

                                                                                                              aaGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCATGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGAAACCGTa                      <  1:832400/140‑1 (MQ=255)

                                                                                                               aGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGAAACCGTag                     >  2:997724/1‑140 (MQ=255)

                                                                                                               aGCCCCGCCGAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGAAACCGTag                     >  2:258501/1‑140 (MQ=255)

                                                                                                                   ccgccgAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGAAACCGTagaaga                 <  2:216238/140‑1 (MQ=255)

                                                                                                                   ccgccgAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGAAACCGTagaaga                 <  1:644402/140‑1 (MQ=255)

                                                                                                                    cgccgAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGa                             <  2:313079/127‑1 (MQ=255)

                                                                                                                    cgccgAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGa                             >  1:313079/1‑127 (MQ=255)

                                                                                                                    cgccgAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGAAACCGTAGAAgat                >  2:1207772/1‑140 (MQ=255)

                                                                                                                     gccgAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGAAACCGTAGAAgatg               <  1:835632/140‑1 (MQ=255)

                                                                                                                     gccgAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGAAACCGTAGAAgatg               <  1:1207772/140‑1 (MQ=255)

                                                                                                                      ccgAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGAAACCGTAGAAgatga              <  2:407979/140‑1 (MQ=255)

                                                                                                                        gAAGCGGGGCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGAAACCGTAGAAGatgatg            <  1:379454/140‑1 (MQ=255)

                                                                                                                             ggggCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGAAACCGTAGAAGATGATGCTGTa       <  2:827223/140‑1 (MQ=255)

                                                                                                                               ggCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGAAACCGTAGAAGATGATGCTGTaca     <  2:96031/140‑1 (MQ=255)

                                                                                                                                gCTAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGAAACCGTAGAAGATGATGCTGTacac    <  1:1064611/140‑1 (MQ=255)

                                                                                                                                  tAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGAAACCGTAGAAGATGATGCTGTAcacca  <  1:809150/140‑1 (MQ=255)

                                                                                                                                  tAAATACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGAAACCGTAGAAGATGATGCTGTAcacca  <  1:427325/140‑1 (MQ=255)

CTCGACGTAGTTGTCGTAGTCTGGAATGCCAATCAACATCTTCGCCGCCTGGCCGAGATATTTTTTTGCCTGTCCTAAGTTACCAAACATAGTTCACTCTGATAAGAACAAAGCCCCGCCGAAGCGGGGCTAAACACGGTTAGTGGTGCGAAGAGATCTTCACGCCGCCTTCCGGGATTGGAACGTACGGTGTTTCTTTGTCAGTACGTTTGTCGCTGTTACGCACCGCAAGCCAGGTTTTGAAACCGTAGAAGATGATGCTGTACACCA  >  NC_000913/4588990‑4589259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A