Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	103,619	G→A	Q155Q (CAG→CAA)	ftsQ →	cell division protein FtsQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	103,619	0	G	A	100.0%	100.4 / NA	29	Q155Q (CAG→CAA)	ftsQ	cell division protein FtsQ
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/9); total (20/9)

CAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGATTTACTCTGAAGGAAGCGGCGATGACCGCGCG  >  NC_000913/103487‑103741

cAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTc                                                                                                                     <  1:719610/140‑1 (MQ=255)

  gAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCa                                                                                                                   >  2:276599/1‑140 (MQ=255)

               tgatgaATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGcc                                                                                                      >  1:246250/1‑140 (MQ=255)

                          aGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAgc                                                                                           <  2:655960/140‑1 (MQ=255)

                           gATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAgcg                                                                                          >  1:615009/1‑140 (MQ=255)

                                   tGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGaa                                                                                  <  1:121862/140‑1 (MQ=255)

                                   tGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGaa                                                                                  >  2:28764/1‑140 (MQ=255)

                                       tGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtgt                                                                              <  1:28764/140‑1 (MQ=255)

                                          atatGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGc                                                                           >  1:799352/1‑140 (MQ=255)

                                               tgCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtgt                                                                              <  2:757466/132‑1 (MQ=255)

                                               tgCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtgt                                                                              >  1:757466/1‑132 (MQ=255)

                                               tgCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGc                                                                      >  1:23933/1‑140 (MQ=255)

                                                     ttGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATcgc                                                                >  1:695507/1‑140 (MQ=255)

                                                             tGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATggg                                                        >  2:642509/1‑140 (MQ=255)

                                                                       aaCATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTg                                              >  1:560302/1‑140 (MQ=255)

                                                                         cATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGc                                            >  1:847367/1‑140 (MQ=255)

                                                                               gTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGa                                      <  1:120264/140‑1 (MQ=255)

                                                                               gTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGa                                      >  1:836261/1‑140 (MQ=255)

                                                                                   aCGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGa                                  >  1:249353/1‑140 (MQ=255)

                                                                                   aCGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGa                                  >  2:449873/1‑140 (MQ=255)

                                                                                   aCGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGa                                  >  1:722025/1‑140 (MQ=255)

                                                                                       ggaaggaaATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGATTTa                              >  2:296789/1‑140 (MQ=255)

                                                                                              aaTACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGATTTACTCTgaa                       <  2:836261/140‑1 (MQ=255)

                                                                                                 aCCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGATTTACTCTgaagga                    >  2:377753/1‑140 (MQ=255)

                                                                                                  ccTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGATTTACTCTgaaggaa                   <  1:276599/140‑1 (MQ=255)

                                                                                                   cTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGATTTACTCTgaaggaag                  >  2:318635/1‑140 (MQ=255)

                                                                                                              cgccAGAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGATTTACTCTGAAGGAAGCGGCGATGAcc       >  1:802905/1‑140 (MQ=255)

                                                                                                                   gAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGATTTACTCTGAAGGAAGCGGCGATGACcgcgcg  >  2:607775/1‑140 (MQ=255)

                                                                                                                   gAACGCACCAGCAAGCAAGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGATTTACTCTGAAGGAAGCGGCGATGACcgcgcg  <  1:296789/140‑1 (MQ=255)

CAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGATTTACTCTGAAGGAAGCGGCGATGACCGCGCG  >  NC_000913/103487‑103741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A