Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	106,748	G→A	T64T (ACG→ACA)	lpxC →	UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	106,748	0	G	A	100.0%	208.6 / NA	58	T64T (ACG→ACA)	lpxC	UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/34); total (24/34)

TACCGGCAAGAAAGTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTC  >  NC_000913/106613‑106884

tACCGGCAAGAAAGTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTc                                                                                                                                      >  2:295055/1‑140 (MQ=255)

   cGGCAAGAAAGTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTgg                                                                                                                                   >  2:206355/1‑140 (MQ=255)

   cGGCAAGAAAGTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTgg                                                                                                                                   <  1:540898/140‑1 (MQ=255)

    ggCAAGAAAGTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGt                                                                                                                                  >  2:48288/1‑140 (MQ=255)

    ggCAAGAAAGTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGt                                                                                                                                  >  2:411852/1‑140 (MQ=255)

       aaGAAAGTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCaa                                                                                                                               <  2:813841/140‑1 (MQ=255)

       aaGAAAGTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCaa                                                                                                                               <  1:700934/140‑1 (MQ=255)

           aaGTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAg                                                                                                                           <  1:782539/140‑1 (MQ=255)

                    tGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTa                                                                                                                  >  2:361226/1‑140 (MQ=255)

                           aCGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGAttt                                                                                                           >  2:472424/1‑140 (MQ=255)

                              cccTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCaa                                                                                                        <  2:864190/140‑1 (MQ=255)

                              cccTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCaa                                                                                                        <  1:472424/140‑1 (MQ=255)

                              cccTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCaa                                                                                                        <  1:295055/140‑1 (MQ=255)

                                cTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAAcc                                                                                                      >  1:22583/1‑140 (MQ=255)

                                  gcgcCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTc                                                                                                          >  1:382475/1‑134 (MQ=255)

                                  gcgcCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTc                                                                                                          <  2:382475/134‑1 (MQ=255)

                                  gcgcCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAAc                                                                                                       >  2:717861/1‑137 (MQ=255)

                                  gcgcCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAAc                                                                                                       <  1:717861/137‑1 (MQ=255)

                                  gcgcCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGt                                                                                                    >  1:428380/1‑140 (MQ=255)

                                   cgcCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGTGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTa                                                                                                   <  2:371101/140‑1 (MQ=255)

                                   cgcCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTa                                                                                                   >  1:393296/1‑140 (MQ=255)

                                      cGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTagag                                                                                                <  2:295379/140‑1 (MQ=255)

                                      cGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTagag                                                                                                <  2:194284/140‑1 (MQ=255)

                                      cGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTagag                                                                                                <  2:22583/140‑1 (MQ=255)

                                      cGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTagag                                                                                                >  1:312557/1‑140 (MQ=255)

                                      cGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTagag                                                                                                <  2:475512/140‑1 (MQ=255)

                                                cGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAAtg                                                                                      <  1:411852/140‑1 (MQ=255)

                                                  gggTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAAtgct                                                                                    >  1:825408/1‑140 (MQ=255)

                                                   ggTCATCTATCGTCGTACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAAtgctg                                                                                   <  1:816365/140‑1 (MQ=255)

                                                             cgtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCggg                                                                         >  1:474805/1‑140 (MQ=255)

                                                               tcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCt                                                                       >  1:165258/1‑140 (MQ=255)

                                                                  cACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTgg                                                                    <  1:146097/140‑1 (MQ=255)

                                                                  cACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTgg                                                                    <  2:165258/140‑1 (MQ=255)

                                                                    ccGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGc                                                                  <  1:206355/140‑1 (MQ=255)

                                                                    ccGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGc                                                                  <  2:68374/140‑1 (MQ=255)

                                                                    ccGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGc                                                                  <  1:626864/140‑1 (MQ=255)

                                                                    ccGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGc                                                                  <  2:393296/140‑1 (MQ=255)

                                                                    ccGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGc                                                                  <  2:474805/140‑1 (MQ=255)

                                                                         ttGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTcgcg                                                                           <  2:485289/126‑1 (MQ=255)

                                                                         ttGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTcgcg                                                                           >  1:485289/1‑126 (MQ=255)

                                                                                         aTTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGaa                                             >  2:726909/1‑140 (MQ=255)

                                                                                           ttCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGt                                           >  1:696303/1‑140 (MQ=255)

                                                                                                       gCCAAATCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGa                               >  1:650383/1‑140 (MQ=255)

                                                                                                            aTCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATcc                          <  1:48288/140‑1 (MQ=255)

                                                                                                            aTCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATcc                          <  2:600487/140‑1 (MQ=255)

                                                                                                            aTCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATcc                          <  1:726909/140‑1 (MQ=255)

                                                                                                             tCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATccc                         <  1:295951/140‑1 (MQ=255)

                                                                                                               tgtgCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGa                       <  2:428380/140‑1 (MQ=255)

                                                                                                                 tgCGTGATACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATc                     >  2:553424/1‑140 (MQ=255)

                                                                                                                       aTACCATGCTCTGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGAc               >  2:581844/1‑140 (MQ=255)

                                                                                                                                tctGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACgg             <  2:28530/133‑1 (MQ=255)

                                                                                                                                tctGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACgg             >  1:28530/1‑133 (MQ=255)

                                                                                                                                  tGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGcgccgc    >  1:53659/1‑140 (MQ=255)

                                                                                                                                  tGTACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGcgccgc    >  1:779049/1‑140 (MQ=255)

                                                                                                                                    tACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTc  <  1:318823/140‑1 (MQ=255)

                                                                                                                                    tACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTc  <  2:53659/140‑1 (MQ=255)

                                                                                                                                    tACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTc  <  2:238740/140‑1 (MQ=255)

                                                                                                                                    tACATGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTc  <  2:101514/140‑1 (MQ=255)

TACCGGCAAGAAAGTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTC  >  NC_000913/106613‑106884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A