Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	147,901	T→C	intergenic (+31/+43)	rpnC → / ← panC	recombination‑promoting nuclease RpnC/pantothenate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	147,901	0	T	C	100.0%	157.1 / NA	44	intergenic (+31/+43)	rpnC/panC	recombination‑promoting nuclease RpnC/pantothenate synthetase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/22); total (22/22)

TTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTACCAGAATTACTGCCCGTTTGCTGGTTTCAGAAAC  >  NC_000913/147769‑148036

ttCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATtaa                                                                                                                                  <  1:534839/140‑1 (MQ=255)

      cAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTg                                                                                                                            <  2:221751/140‑1 (MQ=255)

             ttctGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCAt                                                                                                                     >  2:146103/1‑140 (MQ=255)

              tctGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATc                                                                                                                    <  1:94139/140‑1 (MQ=255)

               ctGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCa                                                                                                                   <  1:728743/140‑1 (MQ=255)

                         gAATGTCTCGGGAAGACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAgcgc                                                                                                         <  2:370735/140‑1 (MQ=255)

                         gAATGTCTCGGGAAGACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAgcgc                                                                                                         <  1:681665/140‑1 (MQ=255)

                         gAATGTCTCGGGAAGACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAgcgc                                                                                                         <  1:208246/140‑1 (MQ=255)

                         gAATGTCTCGGGAAGACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAgcgc                                                                                                         <  2:488401/140‑1 (MQ=255)

                         gAATGTCTCGGGAAGACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTCAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAgcgc                                                                                                         <  1:772806/140‑1 (MQ=255)

                            tGTCTCGGGAAGACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAg                                                                                                      >  1:569551/1‑140 (MQ=255)

                              tctcGGGAAGACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAg                                                                                                      <  1:447775/138‑1 (MQ=255)

                              tctcGGGAAGACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAg                                                                                                      >  2:447775/1‑138 (MQ=255)

                                               agagATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTc                                                                                   <  1:146103/140‑1 (MQ=255)

                                                    tGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCa                                                                              >  2:541528/1‑140 (MQ=255)

                                                      gCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGTCCAtt                                                                            <  2:122552/140‑1 (MQ=255)

                                                      gCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCAtt                                                                            >  1:87097/1‑140 (MQ=255)

                                                                 tctTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGAt                                                                 <  1:679248/140‑1 (MQ=255)

                                                                     gCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAgg                                                             >  1:771524/1‑140 (MQ=255)

                                                                     gCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAgg                                                             <  2:771524/140‑1 (MQ=255)

                                                                                tAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGcc                                                  <  1:541528/140‑1 (MQ=255)

                                                                                        ttAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAgg                                          <  2:87097/140‑1 (MQ=255)

                                                                                         tAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAggc                                         >  2:280270/1‑140 (MQ=255)

                                                                                          aaCCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAggcg                                        <  2:569551/140‑1 (MQ=255)

                                                                                              ttATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTa                                    >  2:172594/1‑140 (MQ=255)

                                                                                              ttATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTa                                    >  2:12408/1‑140 (MQ=255)

                                                                                               tATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAggcg                                        >  2:757515/1‑135 (MQ=255)

                                                                                               tATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAggcg                                        <  1:757515/135‑1 (MQ=255)

                                                                                               tATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTAc                                   >  2:668296/1‑140 (MQ=255)

                                                                                               tATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTAc                                   <  2:161172/140‑1 (MQ=255)

                                                                                                aTTTAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTAcc                                  >  2:602625/1‑140 (MQ=255)

                                                                                                 tttAAGTTACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTACCa                                 >  2:197549/1‑140 (MQ=255)

                                                                                                       ttACCTGTGTTATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTACCAGAATTa                           >  2:25137/1‑140 (MQ=255)

                                                                                                                ttATGACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTACCAGAATTACTGCCCGtt                  >  1:45940/1‑140 (MQ=255)

                                                                                                                    gACAGATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTACCAGAATTACTGCCCGTTTGCt              >  1:238968/1‑140 (MQ=255)

                                                                                                                        gaggACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGGCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTACCAGAATTACTGCCCGTTTGCTGGtt          >  2:505447/4‑140 (MQ=255)

                                                                                                                        gATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTACCAGAATTACTGCCCGTTTGCTGGtt          >  2:591297/1‑140 (MQ=255)

                                                                                                                        gATGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTACCAGAATTACTGCCCGTTTGCTGGtt          >  2:177090/1‑140 (MQ=255)

                                                                                                                         aTGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTACCAGAATTACTGCCCGTTTGCTGGttt         >  2:512444/1‑140 (MQ=255)

                                                                                                                         aTGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTACCAGAATTACTGCCCGTTTGCTGGttt         >  2:560910/1‑140 (MQ=255)

                                                                                                                          tGACGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCCGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTACCAGAATTACTGCCCGTTTGCTGGTTTc        >  2:275404/1‑140 (MQ=255)

                                                                                                                            aCGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTACCAGAATTACTGCCCGTTTGCTGGTTTCAg      <  1:151726/140‑1 (MQ=255)

                                                                                                                             cGTGGGGCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTACCAGAATTACTGCCCGTTTGCTGGTTTCAGa     <  1:177090/140‑1 (MQ=255)

                                                                                                                                ggggCAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTACCAGAATTACTGCCCGTTTGCTGGTTTCAGAAAc  <  2:626958/140‑1 (MQ=255)

TTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTCGATCAGGCGAGCATCGCCAAGCCAGGCGGCTACCAGAATTACTGCCCGTTTGCTGGTTTCAGAAAC  >  NC_000913/147769‑148036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A