Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 227 52.0 1764248 99.3% 1751898 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 154,784 C→T A215T (GCC→ACC)  htrE ← putative fimbrial usher protein HtrE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913154,7840CT97.0% 218.9 / ‑4.7 67A215T (GCC→ACC) htrEputative fimbrial usher protein HtrE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (32/33);  minor base A (2/0);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAGGTTGTATGACAACATGGCCGCATTAATGCCGTTTTCCCA  >  NC_000913/154647‑154919
                                                                                                                                         |                                                                                                                                       
aTCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTAc                                                                                                                                       >  1:233564/1‑140 (MQ=255)
     aGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGt                                                                                                                                  >  1:733091/1‑140 (MQ=255)
         ttCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGcc                                                                                                                              >  1:217023/1‑140 (MQ=255)
         ttCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGcc                                                                                                                              <  1:398122/140‑1 (MQ=255)
          tCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCGAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCa                                                                                                                             <  2:825239/140‑1 (MQ=255)
          tCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCa                                                                                                                             <  2:668625/140‑1 (MQ=255)
          tCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCa                                                                                                                             <  2:852367/140‑1 (MQ=255)
          ccGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCa                                                                                                                             <  2:75517/139‑1 (MQ=255)
           cGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCAt                                                                                                                            <  2:733091/140‑1 (MQ=255)
           cGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCAt                                                                                                                            >  1:286854/1‑140 (MQ=255)
            gCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATg                                                                                                                           <  2:231963/140‑1 (MQ=255)
                  cgtATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTAGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTa                                                                                                                     >  2:611294/1‑140 (MQ=255)
                           cTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATcc                                                                                                            <  2:179739/140‑1 (MQ=255)
                            tCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATccc                                                                                                           >  1:66199/1‑140 (MQ=255)
                            tCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATccc                                                                                                           <  2:211259/140‑1 (MQ=255)
                            tCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATccc                                                                                                           >  2:867979/1‑140 (MQ=255)
                             cACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCa                                                                                                          <  2:286854/140‑1 (MQ=255)
                              aCCAAGATTGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCAc                                                                                                         >  1:789382/1‑140 (MQ=255)
                              aCCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCa                                                                                                          <  2:167550/139‑1 (MQ=255)
                              aCCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCa                                                                                                          >  1:167550/1‑139 (MQ=255)
                              aCCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCAc                                                                                                         >  1:34210/1‑140 (MQ=255)
                                       gAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATg                                                                                                <  2:190971/140‑1 (MQ=255)
                                        aGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGAACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATgc                                                                                               >  2:532691/1‑140 (MQ=255)
                                              gaACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATa                                                                                         >  1:271806/1‑140 (MQ=255)
                                                  gCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATg                                                                                     >  2:62332/1‑140 (MQ=255)
                                                    aGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCt                                                                                   >  2:740714/1‑140 (MQ=255)
                                                                tCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCg                                                                       >  1:510034/1‑140 (MQ=255)
                                                                   cGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGAcc                                                                    <  1:867979/140‑1 (MQ=255)
                                                                     cTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAg                                                                  >  2:438764/1‑140 (MQ=255)
                                                                        aaCATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAgggg                                                               <  1:740714/140‑1 (MQ=255)
                                                                        aaCATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAgggg                                                               <  1:438764/140‑1 (MQ=255)
                                                                          cATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGtt                                                             >  1:34998/1‑140 (MQ=255)
                                                                           aTACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGttt                                                            <  1:1901/140‑1 (MQ=255)
                                                                             aCCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAgggg                                                               <  2:7326/135‑1 (MQ=255)
                                                                             aCCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAgggg                                                               >  1:7326/1‑135 (MQ=255)
                                                                             aCCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCa                                                          <  2:871698/140‑1 (MQ=255)
                                                                               cGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACt                                                        <  2:855855/140‑1 (MQ=255)
                                                                               cGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACt                                                        <  1:472774/140‑1 (MQ=255)
                                                                                gATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTa                                                       <  2:233564/140‑1 (MQ=255)
                                                                                  ttCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATg                                                     >  2:714162/1‑140 (MQ=255)
                                                                                  ttCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATg                                                     >  1:455905/1‑140 (MQ=255)
                                                                                  ttCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATg                                                     >  1:614131/1‑140 (MQ=255)
                                                                                  ttCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATg                                                     >  1:810277/1‑140 (MQ=255)
                                                                                    cTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGat                                                   <  2:34998/140‑1 (MQ=255)
                                                                                    cTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGat                                                   <  2:616722/140‑1 (MQ=255)
                                                                                       aaaaTCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCa                                                          <  2:46858/130‑1 (MQ=255)
                                                                                       aaaaTCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCa                                                          >  1:46858/1‑130 (MQ=255)
                                                                                       aaaaTCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATc                                                <  2:789382/140‑1 (MQ=255)
                                                                                             aTAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGa                                          <  2:455905/140‑1 (MQ=255)
                                                                                             aTAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGa                                          <  2:614131/140‑1 (MQ=255)
                                                                                              tAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCA‑TTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAgg                                        <  1:69428/140‑1 (MQ=255)
                                                                                               aaTTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAgg                                        <  1:62332/140‑1 (MQ=255)
                                                                                                 ttACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAGGtt                                      <  2:510034/140‑1 (MQ=255)
                                                                                                  tACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAGGTTg                                     <  2:66199/140‑1 (MQ=255)
                                                                                                     tGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAGGTTGTAt                                  >  2:128708/1‑140 (MQ=255)
                                                                                                           aaTCGGTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAGGTTGTATGacaac                            >  2:396810/1‑140 (MQ=255)
                                                                                                           aaTCGGTCATCCAGTTGTAGTTGCCCGAGGAACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAGGTTGTATGacaac                            >  1:182277/1‑140 (MQ=255)
                                                                                                               ggTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAGGTTg                                     <  2:287361/127‑1 (MQ=255)
                                                                                                               ggTCATCCAGTTGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAGGTTg                                     >  1:287361/1‑127 (MQ=255)
                                                                                                                 tCATCCAGTTGTAGTTGCCCGAGGTACGCGGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAGGTTGTATGACAACATGGcc                      >  2:799295/1‑140 (MQ=255)
                                                                                                                         ttGTAGTTGCCCGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAGGTTGTATGACAACATGGCCGCATTAAt              >  1:521973/1‑140 (MQ=255)
                                                                                                                                  cccGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATCTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAGGTTGTATGACAACATGGCCGCATTAATGCCGTTTTc     >  1:4241/1‑140 (MQ=255)
                                                                                                                                   ccGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCTGGGGTTTCACTATGATATCCGTTGAGGTTGTATGACAACATGGCCGCATTAATGCCGTTTTcc    >  2:68247/1‑140 (MQ=255)
                                                                                                                                   ccGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAGGTTGTATGACAACATGGCCGCATTAATGCCGTTTTcc    >  2:103596/1‑140 (MQ=255)
                                                                                                                                   ccGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAGGTTGTATGACAACATGGCCGCATTAATGCCGTTTTcc    >  1:820747/1‑140 (MQ=255)
                                                                                                                                   ccGAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAGGTTGTATGACAACATGGCCGCATTAATGCCGTTTTcc    >  1:185841/1‑140 (MQ=255)
                                                                                                                                     gAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAGGTTGTATGACAACATGGCCGCATTAATGCCGTTTTCCCa  <  2:185841/140‑1 (MQ=255)
                                                                                                                                     gAGGTACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAGGTTGTATGACAACATGGCCGCATTAATGCCGTTTTCCCa  <  2:182277/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
ATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGCCATGCACCTAAATTCATCCCACCGTTAAATGCAGCATAAATGCTTTCATTTTTTCGACCAGGGGTTTCACTATGATATCCGTTGAGGTTGTATGACAACATGGCCGCATTAATGCCGTTTTCCCA  >  NC_000913/154647‑154919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A