Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	156,672	T→C	D71G (GAC→GGC)	yadN ←	putative fimbrial protein YadN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	156,672	0	T	C	98.9%	305.7 / ‑4.6	88	D71G (GAC→GGC)	yadN	putative fimbrial protein YadN
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (37/50); total (37/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01

TGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTC  >  NC_000913/156535‑156808

tGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCa                                                                                                                                        >  2:247961/1‑140 (MQ=255)

tGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCa                                                                                                                                        >  2:405798/1‑140 (MQ=255)

tGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCa                                                                                                                                        >  2:48135/1‑140 (MQ=255)

tGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCa                                                                                                                                        >  2:629249/1‑140 (MQ=255)

tGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCa                                                                                                                                        >  2:820970/1‑140 (MQ=255)

tGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCa                                                                                                                                        >  1:303776/1‑140 (MQ=255)

 gTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCa                                                                                                                                        >  2:129833/1‑139 (MQ=255)

 gTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCAt                                                                                                                                       >  2:425400/1‑140 (MQ=255)

  tCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCAtt                                                                                                                                      >  1:690118/1‑140 (MQ=255)

   cGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTc                                                                                                                                     >  1:484754/1‑140 (MQ=255)

     ttgttCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAg                                                                                                                                   <  1:425400/140‑1 (MQ=255)

          cAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCGGTACGc                                                                                                                              >  1:126495/1‑140 (MQ=255)

          cAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGc                                                                                                                              <  1:161090/140‑1 (MQ=255)

              gTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGc                                                                                                                          >  2:743546/1‑140 (MQ=255)

               tCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCa                                                                                                                         <  2:7887/140‑1 (MQ=255)

               tCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCa                                                                                                                         <  1:263175/140‑1 (MQ=255)

               tCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCa                                                                                                                         <  1:232207/140‑1 (MQ=255)

               tCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTACTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCa                                                                                                                         >  2:767935/1‑140 (MQ=255)

                  cTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCg                                                                                                                      >  2:860700/1‑140 (MQ=255)

                  cTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCg                                                                                                                      >  2:514779/1‑140 (MQ=255)

                          ttACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCAcc                                                                                                              <  2:162526/140‑1 (MQ=255)

                          ttACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCAcc                                                                                                              <  1:767935/140‑1 (MQ=255)

                           tACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCa                                                                                                             >  1:54725/1‑140 (MQ=255)

                                 aGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGtt                                                                                                       >  1:879830/1‑140 (MQ=255)

                                 aGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGtt                                                                                                       >  1:208282/1‑140 (MQ=255)

                                    aaaCAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAGGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCg                                                                                                    <  2:726274/140‑1 (MQ=255)

                                    aaaCAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCg                                                                                                    >  2:429775/1‑140 (MQ=255)

                                    aaaCAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCg                                                                                                    >  2:786002/1‑140 (MQ=255)

                                     aaCAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCaa                                                                                                            <  2:439860/131‑1 (MQ=255)

                                     aaCAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCaa                                                                                                            >  1:439860/1‑131 (MQ=255)

                                       cAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGtt                                                                                                 <  1:247961/140‑1 (MQ=255)

                                        aGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGttt                                                                                                <  2:558128/140‑1 (MQ=255)

                                            ccGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTgcag                                                                                            <  1:215919/140‑1 (MQ=255)

                                            ccGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTgcag                                                                                            <  1:702661/140‑1 (MQ=255)

                                            ccGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTgcag                                                                                            <  2:30133/140‑1 (MQ=255)

                                             cGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTgcagc                                                                                           <  2:600537/140‑1 (MQ=255)

                                             cGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTgcagc                                                                                           <  2:208282/140‑1 (MQ=255)

                                             cGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTgcagc                                                                                           <  1:429775/140‑1 (MQ=255)

                                                 ggTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGa                                                                                       <  1:405798/140‑1 (MQ=255)

                                                  gTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGAt                                                                                      <  2:303776/140‑1 (MQ=255)

                                                    cATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCg                                                                                                    >  1:174284/1‑124 (MQ=255)

                                                    cATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCg                                                                                                    <  2:174284/124‑1 (MQ=255)

                                                    cATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCa                                                                                    >  1:8996/1‑140 (MQ=255)

                                                      ttttAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGa                                                                                  <  1:134880/140‑1 (MQ=255)

                                                        ttAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGAcc                                                                                <  2:879830/140‑1 (MQ=255)

                                                        ttAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGAcc                                                                                <  2:54725/140‑1 (MQ=255)

                                                            ccGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATcc                                                                            >  2:66813/1‑140 (MQ=255)

                                                                 cTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGtt                                                                       >  2:560129/1‑140 (MQ=255)

                                                                     cTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTAccg                                                                   <  2:622467/140‑1 (MQ=255)

                                                                         aTTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGt                                                               >  1:345290/1‑140 (MQ=255)

                                                                         aTTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGt                                                               >  1:474967/1‑140 (MQ=255)

                                                                            cGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCaa                                                            >  2:741072/1‑140 (MQ=255)

                                                                              gATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAAcg                                                          >  1:803933/1‑140 (MQ=255)

                                                                                tttgctttgcTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAAcgcg                                                        <  1:460838/140‑1 (MQ=255)

                                                                                tttgctttgcTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAAcgcg                                                        <  1:48135/140‑1 (MQ=255)

                                                                                tttgctttgcTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAAcgcg                                                        <  2:8996/140‑1 (MQ=255)

                                                                                tttgctttgcTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAAcgcg                                                        <  1:820970/140‑1 (MQ=255)

                                                                                  tgctttgctGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCCCCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCggg                                                      <  2:174971/140‑1 (MQ=255)

                                                                                       tgctgcAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTca                                                 <  1:866432/140‑1 (MQ=255)

                                                                                       tgctgcAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTca                                                 <  1:66813/140‑1 (MQ=255)

                                                                                       tgctgcAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTca                                                 <  1:514779/140‑1 (MQ=255)

                                                                                          tgcAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAg                                              <  2:513460/140‑1 (MQ=255)

                                                                                          tgcAGTAAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAg                                              <  2:256642/140‑1 (MQ=255)

                                                                                              gTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGgtgt                                          <  2:484754/140‑1 (MQ=255)

                                                                                              gTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGgtgt                                          <  2:348009/140‑1 (MQ=255)

                                                                                              gTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGgtgt                                          >  1:268509/1‑140 (MQ=255)

                                                                                                 aaCGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTca                                                 <  2:756123/130‑1 (MQ=255)

                                                                                                 aaCGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTca                                                 >  1:756123/1‑130 (MQ=255)

                                                                                                  aCGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATc                                      >  1:458796/1‑140 (MQ=255)

                                                                                                   cGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATca                                     <  2:474967/140‑1 (MQ=255)

                                                                                                   cGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATca                                     <  1:860700/140‑1 (MQ=255)

                                                                                                     gTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATcaac                                   <  2:690118/140‑1 (MQ=255)

                                                                                                     gTGATGCTGAAAGGTTTAGCTCTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATcaac                                   <  2:803933/140‑1 (MQ=255)

                                                                                                        aTGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCaacaac                                <  2:345290/140‑1 (MQ=255)

                                                                                                        aTGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCaacaac                                <  1:363704/140‑1 (MQ=255)

                                                                                                        aTGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCaacaac                                >  2:721210/1‑140 (MQ=255)

                                                                                                          gCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCGGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCa                              <  2:126495/140‑1 (MQ=255)

                                                                                                          gCTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCa                              <  1:741072/140‑1 (MQ=255)

                                                                                                           cTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAg                                              >  1:470522/1‑123 (MQ=255)

                                                                                                           cTGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAg                                              <  2:470522/123‑1 (MQ=255)

                                                                                                            tGAAAGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGa                            <  2:646600/140‑1 (MQ=255)

                                                                                                              aaaGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGAcc                          >  2:664276/1‑140 (MQ=255)

                                                                                                                aGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCAc                        <  2:592894/140‑1 (MQ=255)

                                                                                                                aGGTTTAGCTTTAGCGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCAc                        <  1:743546/140‑1 (MQ=255)

                                                                                                                  gTTTAGCTTTAGCGCCAAGGGTGCCATTCAGTACGCCAGCATCGATTTCTCCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTg                      <  1:629249/140‑1 (MQ=255)

                                                                                                                     tagctttagcGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACt                       <  2:180993/136‑1 (MQ=255)

                                                                                                                     tagctttagcGCCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACt                       >  1:180993/1‑136 (MQ=255)

                                                                                                                              cgcCAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGa          <  1:786002/140‑1 (MQ=255)

                                                                                                                                ccAACGGTGCCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGGcc        >  2:801483/1‑140 (MQ=255)

                                                                                                                                 cAACGGTGCCATTCAGTACGCCGGAATAGATTTCCCCAACGGTTGCGGTTTGCAGCGGGATCAGACCACCTTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGAccg       <  2:210652/140‑1 (MQ=255)

                                                                                                                                      gtgcCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGGCCGCCGTc  <  1:423710/140‑1 (MQ=255)

TGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTC  >  NC_000913/156535‑156808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A