Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	209,049	A→G	A143A (GCA→GCG)	accA →	acetyl‑CoA carboxyltransferase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	209,049	0	A	G	100.0%	141.2 / NA	41	A143A (GCA→GCG)	accA	acetyl‑CoA carboxyltransferase subunit alpha
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/23); total (18/23)

GATAAAGCTATCGTCGGTGGTATCGCCCGTCTCGATGGTCGTCCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGT  >  NC_000913/208912‑209178

gATAAAGCTATCGTCGGTGGTATCGCCCGTCTCGATGGTCGTCCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCt                                                                                                                                 >  2:481324/1‑140 (MQ=255)

                gtggtATCGCCCGTCTCGATGGTCGTCCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGt                                                                                                                             >  2:748374/1‑128 (MQ=255)

                gtggtATCGCCCGTCTCGATGGTCGTCCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGt                                                                                                                             <  1:748374/128‑1 (MQ=255)

                 tggtATCGCCCGTCTCGATGGTCGTCCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATgg                                                                                                                >  1:673007/1‑140 (MQ=255)

                     aTCGCCCGTCTCGATGGTCGTCCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGa                                                                                                            <  2:531987/140‑1 (MQ=255)

                      tCGCCCGTCTCGATGGTCGTCCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGaa                                                                                                           <  2:304654/140‑1 (MQ=255)

                      tCGCCCGTCTCGATGGTCGTCCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGaa                                                                                                           <  2:595725/140‑1 (MQ=255)

                         cccGTCTCGATGGTCGTCCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGaa                                                                                                           <  1:114314/137‑1 (MQ=255)

                         cccGTCTCGATGGTCGTCCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGaa                                                                                                           >  2:114314/1‑137 (MQ=255)

                           cGTCTCGATGGTCGTCCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCtt                                                                                                      >  1:665168/1‑140 (MQ=255)

                              ctcGATGGTCGTCCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTaa                                                                                                   >  1:130145/1‑140 (MQ=255)

                                cGAAGGTCGTCCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGa                                                                                                 <  2:800319/140‑1 (MQ=255)

                                   tGGTCGTCCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGc                                                                                              <  2:665168/140‑1 (MQ=255)

                                   tGGTCGTCCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGc                                                                                              <  2:673007/140‑1 (MQ=255)

                                   tGGTCGTCCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGc                                                                                              <  2:522156/140‑1 (MQ=255)

                                        gtcCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCt                                                                                                       >  2:242505/1‑126 (MQ=255)

                                        gtcCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCt                                                                                                       <  1:242505/126‑1 (MQ=255)

                                        gtcCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTatc                                                                                         >  1:503479/1‑140 (MQ=255)

                                         tcCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTatca                                                                                        >  1:324762/1‑140 (MQ=255)

                                            ggTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTAtcatca                                                                                     <  2:130145/140‑1 (MQ=255)

                                                atgatCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCtt                                                                                 <  2:503479/140‑1 (MQ=255)

                                                   atCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTAt                                                                              >  1:490250/1‑140 (MQ=255)

                                                      aTTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGa                                                                           <  1:481324/140‑1 (MQ=255)

                                                      aTTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGa                                                                           <  1:337943/140‑1 (MQ=255)

                                                             atcaAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCg                                                                    >  2:637147/1‑140 (MQ=255)

                                                                  aaaGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGc                                                               <  2:664309/140‑1 (MQ=255)

                                                                  aaaGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGc                                                               <  2:196251/140‑1 (MQ=255)

                                                                     ggTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTa                                                            >  2:96669/1‑140 (MQ=255)

                                                                        cGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGTCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATcc                                                         >  2:519881/1‑140 (MQ=255)

                                                                         gTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCt                                                        >  2:366253/1‑140 (MQ=255)

                                                                         gTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCt                                                        >  2:881233/1‑140 (MQ=255)

                                                                              aCCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGt                                                   <  1:51462/140‑1 (MQ=255)

                                                                                      aaaaaTTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCag                                           <  1:881233/140‑1 (MQ=255)

                                                                                                            aTGTCAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGc                     <  1:519881/140‑1 (MQ=255)

                                                                                                                cAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCAt                  >  1:739672/1‑139 (MQ=255)

                                                                                                                cAGCGCCAGAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCAt                  <  2:739672/139‑1 (MQ=255)

                                                                                                                        gAAGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCaa         <  1:3736/140‑1 (MQ=255)

                                                                                                                          aGGTTACCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACCCCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAAcc       <  2:312769/140‑1 (MQ=255)

                                                                                                                               aCCGCAAAGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCGATTGCACGCAACCTGCGt  >  1:439406/1‑140 (MQ=255)

                                                                                                                                     aaGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCg   <  2:492273/133‑1 (MQ=255)

                                                                                                                                     aaGCGCTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCg   >  1:492273/1‑133 (MQ=255)

GATAAAGCTATCGTCGGTGGTATCGCCCGTCTCGATGGTCGTCCGGTGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGT  >  NC_000913/208912‑209178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A