Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,452,425	T→C	A406A (GCA→GCG)	paaZ ←	oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,452,425	0	T	C	100.0%	154.6 / NA	45	A406A (GCA→GCG)	paaZ	oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/25); total (20/25)

GGATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATAAATCC  >  NC_000913/1452289‑1452545

ggATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCt                                                                                                                       >  2:292125/1‑140 (MQ=255)

ggATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCt                                                                                                                       >  1:618464/1‑140 (MQ=255)

           cGCCCATGCGTACGTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGt                                                                                                            <  2:82544/140‑1 (MQ=255)

           cGCCCATGCGTACGTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGt                                                                                                            >  1:301716/1‑140 (MQ=255)

            gCCCATGTGTACGTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGtt                                                                                                           >  1:291407/1‑140 (MQ=255)

            gCCCATGCGTACGTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGtt                                                                                                           <  2:22427/140‑1 (MQ=255)

              ccATGCGTACGTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGACCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGc                                                                                                         >  2:617980/1‑140 (MQ=255)

                 tGCGTACGTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGAc                                                                                                      <  1:281016/140‑1 (MQ=255)

                        gTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTATCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCa                                                                                               <  2:617464/140‑1 (MQ=255)

                            cgcgTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAgg                                                                                           <  1:273695/140‑1 (MQ=255)

                            cgcgTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAgg                                                                                           >  1:876032/1‑140 (MQ=255)

                            cgcgTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAgg                                                                                           >  2:401559/1‑140 (MQ=255)

                              cgTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCt                                                                                         <  1:466973/140‑1 (MQ=255)

                               gTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCtt                                                                                        <  2:618464/140‑1 (MQ=255)

                                  ggCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTg                                                                                     <  1:401559/140‑1 (MQ=255)

                                    cAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGtt                                                                                   <  1:377989/140‑1 (MQ=255)

                                              aCGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTAccg                                                                         >  1:590476/1‑140 (MQ=255)

                                              aCGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTAccg                                                                         >  2:359360/1‑140 (MQ=255)

                                              aCGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTAccg                                                                         >  1:185141/1‑140 (MQ=255)

                                              aCGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCATCTGTTGCATGTAccg                                                                         >  2:792901/1‑140 (MQ=255)

                                                 cgcAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTAccgccg                                                                      >  1:47660/1‑140 (MQ=255)

                                                        tGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTc                                                               >  2:255433/1‑140 (MQ=255)

                                                              tCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCgg                                                         >  1:842076/1‑140 (MQ=255)

                                                                aGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGACCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCt                                                       <  1:617980/140‑1 (MQ=255)

                                                                   ctcccccAGCGTCCCCCAAAGCCTCCCCCCCCCTCCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCg                                                    <  2:876032/135‑1 (MQ=255)

                                                                     tCACCAGCGTTCCCGCAAGGCTCCCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGa                                                  <  1:674308/140‑1 (MQ=255)

                                                                     tCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGa                                                  <  2:185141/140‑1 (MQ=255)

                                                                     tCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGa                                                  <  1:279039/140‑1 (MQ=255)

                                                                     tCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGa                                                  <  1:292125/140‑1 (MQ=255)

                                                                     tCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGa                                                  <  2:763215/140‑1 (MQ=255)

                                                                     tCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGa                                                  <  2:291407/140‑1 (MQ=255)

                                                                       aCCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACa                                                >  2:45488/1‑140 (MQ=255)

                                                                       aCCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACa                                                <  2:842076/140‑1 (MQ=255)

                                                                        ccAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAg                                               <  1:588036/140‑1 (MQ=255)

                                                                            cGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCgg                                                         <  2:719929/126‑1 (MQ=255)

                                                                            cGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCgg                                                         >  1:719929/1‑126 (MQ=255)

                                                                            cGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAGTACa                                           >  2:358904/1‑140 (MQ=255)

                                                                                       ggCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGc                                <  1:749952/140‑1 (MQ=255)

                                                                                                cgccTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGaagaag                       <  1:391391/140‑1 (MQ=255)

                                                                                                 gccTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCggg                             >  2:795833/1‑133 (MQ=255)

                                                                                                 gccTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCggg                             <  1:795833/133‑1 (MQ=255)

                                                                                                          aGCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCgcag             <  1:358904/140‑1 (MQ=255)

                                                                                                           gCCAGTTGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCgcagc            >  2:381622/1‑140 (MQ=255)

                                                                                                                 tGCAGAGCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATaa      <  2:47660/140‑1 (MQ=255)

                                                                                                                     gagCATGTCGCTGGTTTTGCGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATAAATcc  >  1:552226/1‑140 (MQ=255)

GGATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGCTACCGCCGCCTGCACAAGCCAGTTGCAGAGCATGTCGCTGGTTTTGTGCTGGCATCAGCGTTGCGACAGGGCCAAAGGCTTCTGTTGCATGTACCGCCGGTGTTTCATCCGGCTGCGGACAGTACAATAAGGTTGGCGGGAAGAAGGCACCCGCAGCAGATAAATCC  >  NC_000913/1452289‑1452545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A