Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,512,239	T→G	L196V (TTG→GTG)	ydcS →	putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,512,239	0	T	G	100.0%	226.2 / NA	64	L196V (TTG→GTG)	ydcS	putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (32/32); total (32/32)

ACAACACTAAAACCTTCCCGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACT  >  NC_000913/1512105‑1512370

acaacaCTAAAACCTTCCCGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTc                                                                                                                                <  2:646135/140‑1 (MQ=255)

       tAAAACCTTCCCGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCGTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAg                                                                                                                         >  2:611558/1‑140 (MQ=255)

       tAAAACCTTCCCGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAg                                                                                                                         >  1:629046/1‑140 (MQ=255)

            ccTTCCCGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACt                                                                                                                    <  1:308744/140‑1 (MQ=255)

               tCCCGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAg                                                                                                                 >  2:875071/1‑140 (MQ=255)

               tCCCGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAg                                                                                                                 >  2:264869/1‑140 (MQ=255)

                  cGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCg                                                                                                              <  1:494304/140‑1 (MQ=255)

                     cgccgcCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAg                                                                                                           <  2:501010/140‑1 (MQ=255)

                        cgccgGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTg                                                                                                        <  1:809777/140‑1 (MQ=255)

                          ccgGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTggg                                                                                                      >  2:180735/1‑140 (MQ=255)

                                   tGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCTCTCAGCCGCAGTTGGGCATCAGCGa                                                                                             <  1:649289/140‑1 (MQ=255)

                                   tGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGa                                                                                             <  2:168103/140‑1 (MQ=255)

                                            gTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCa                                                                                    <  1:875071/140‑1 (MQ=255)

                                               tttGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATTAGCGATCCGTATCAACt                                                                                 <  1:789651/140‑1 (MQ=255)

                                               tttGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACt                                                                                 >  1:56167/1‑140 (MQ=255)

                                                    tgAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCg                                                                            <  1:529239/140‑1 (MQ=255)

                                                    tgAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCg                                                                            >  1:777416/1‑140 (MQ=255)

                                                         aaaaTCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCgaagaa                                                                       <  2:629046/140‑1 (MQ=255)

                                                                   ggACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAgg                                                             <  2:475591/140‑1 (MQ=255)

                                                                    gACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGc                                                            <  1:264869/140‑1 (MQ=255)

                                                                     aCGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCg                                                           <  1:611558/140‑1 (MQ=255)

                                                                                aaTAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGt                                                >  2:111725/1‑140 (MQ=255)

                                                                                 aTAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGtg                                               >  2:836200/1‑140 (MQ=255)

                                                                                     aGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGtgctgc                                           <  1:111725/140‑1 (MQ=255)

                                                                                       gCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTgcgc                                         >  1:415987/1‑140 (MQ=255)

                                                                                       gCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTgcgc                                         <  2:777416/140‑1 (MQ=255)

                                                                                       gCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTgcgc                                         <  1:836200/140‑1 (MQ=255)

                                                                                       gCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTgcgc                                         <  2:739476/140‑1 (MQ=255)

                                                                                       gCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTgcgc                                         >  1:537693/1‑140 (MQ=255)

                                                                                          gcgTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCt                                      <  1:769160/140‑1 (MQ=255)

                                                                                          gcgTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCt                                      <  1:57490/140‑1 (MQ=255)

                                                                                            gTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCa                                    >  2:847214/1‑140 (MQ=255)

                                                                                            gTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCa                                    >  1:536421/1‑140 (MQ=255)

                                                                                                      atgatgGCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTg                          >  1:471394/1‑140 (MQ=255)

                                                                                                            gCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCAt                    <  1:417973/140‑1 (MQ=255)

                                                                                                            gCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCAt                    >  2:182480/1‑140 (MQ=255)

                                                                                                            gCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCAt                    >  1:745471/1‑140 (MQ=255)

                                                                                                            gCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCAt                    >  2:848354/1‑140 (MQ=255)

                                                                                                            gCCCTATCTATATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCAt                    >  1:693295/1‑140 (MQ=255)

                                                                                                                tatctatATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCt                >  2:256421/1‑140 (MQ=255)

                                                                                                                 atctatATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTa               <  2:537693/140‑1 (MQ=255)

                                                                                                                 atctatATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTa               <  2:536421/140‑1 (MQ=255)

                                                                                                                 atctatATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTa               >  2:495807/1‑140 (MQ=255)

                                                                                                                 atctatATTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTa               <  2:415987/140‑1 (MQ=255)

                                                                                                                     atatTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTgg           <  1:847214/140‑1 (MQ=255)

                                                                                                                     atatTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTgg           <  2:693295/140‑1 (MQ=255)

                                                                                                                     atatTGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTgg           <  1:256421/140‑1 (MQ=255)

                                                                                                                        ttGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTa               <  1:100486/133‑1 (MQ=255)

                                                                                                                        ttGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTa               >  2:100486/1‑133 (MQ=255)

                                                                                                                        ttGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTgg           <  2:307099/137‑1 (MQ=255)

                                                                                                                        ttGCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTgg           >  1:307099/1‑137 (MQ=255)

                                                                                                                          gCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGa      >  1:535568/1‑140 (MQ=255)

                                                                                                                          gCGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGa      <  1:127709/140‑1 (MQ=255)

                                                                                                                           cGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGac     >  2:753071/1‑140 (MQ=255)

                                                                                                                           cGGACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGac     >  1:57737/1‑140 (MQ=255)

                                                                                                                            ggACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTTAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGaca    >  1:726321/1‑140 (MQ=255)

                                                                                                                            ggACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGaca    >  2:123837/1‑140 (MQ=255)

                                                                                                                            ggACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGaca    >  1:647188/1‑140 (MQ=255)

                                                                                                                             gACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTgg           >  2:519101/1‑132 (MQ=255)

                                                                                                                             gACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTgg           >  2:689603/1‑132 (MQ=255)

                                                                                                                             gACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTgg           <  1:519101/132‑1 (MQ=255)

                                                                                                                             gACGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTgg           <  1:689603/132‑1 (MQ=255)

                                                                                                                              aCGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACt  >  1:218874/1‑140 (MQ=255)

                                                                                                                              aCGCTGCGGTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACt  >  2:8667/1‑140 (MQ=255)

ACAACACTAAAACCTTCCCGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACT  >  NC_000913/1512105‑1512370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A