Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,541,596	C→T	R332H (CGC→CAC)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,541,596	0	C	T	100.0%	130.0 / NA	40	R332H (CGC→CAC)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/20); total (20/20)

TACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGC  >  NC_000913/1541460‑1541726

tACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCggtggc                                                                                                                                 >  2:778285/1‑140 (MQ=255)

tACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCggtggc                                                                                                                                 >  2:495322/1‑140 (MQ=255)

  cGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCag                                                                                                                               >  2:727178/1‑140 (MQ=255)

    gTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCagta                                                                                                                             <  1:575725/140‑1 (MQ=255)

    gTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCagta                                                                                                                             <  1:84488/140‑1 (MQ=255)

               cGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAagt                                                                                                                  >  1:461611/1‑140 (MQ=255)

               cGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAagt                                                                                                                  >  2:206610/1‑140 (MQ=255)

                     gttgCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGc                                                                                                            <  1:727178/140‑1 (MQ=255)

                        gCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTgg                                                                                                         <  2:877114/140‑1 (MQ=255)

                                ccAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGGAGTCGCTGGGATTATCg                                                                                                 >  1:536313/1‑140 (MQ=255)

                                  aGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCgag                                                                                               >  1:401519/1‑140 (MQ=255)

                                        tccaCCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGaaa                                                                                         <  2:461611/140‑1 (MQ=255)

                                                gTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTa                                                                                 >  2:366975/1‑140 (MQ=255)

                                                         gcgGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACAt                                                                        <  1:450432/140‑1 (MQ=255)

                                                         gcgGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACAt                                                                        <  1:495322/140‑1 (MQ=255)

                                                               cATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCa                                                                  <  1:206610/140‑1 (MQ=255)

                                                                   cgcCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGc                                                              >  1:333568/1‑140 (MQ=255)

                                                                       cGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATc                                                          >  1:758533/1‑140 (MQ=255)

                                                                              gTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAggg                                                   <  2:333568/140‑1 (MQ=255)

                                                                                aGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGgcg                                                 <  1:366975/140‑1 (MQ=255)

                                                                                    aCCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTAt                                             >  2:539949/1‑140 (MQ=255)

                                                                                       aTCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAg                                          >  2:381434/1‑140 (MQ=255)

                                                                                          gtcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCa                                                      <  1:644623/125‑1 (MQ=255)

                                                                                          gtcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCa                                                      >  2:644623/1‑125 (MQ=255)

                                                                                          gtcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGc                                       >  1:862577/1‑140 (MQ=255)

                                                                                           tcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGgc                                                  <  2:89698/128‑1 (MQ=255)

                                                                                           tcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGgc                                                  >  1:89698/1‑128 (MQ=255)

                                                                                           tcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGcc                                      >  2:518021/1‑140 (MQ=255)

                                                                                           tcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGcc                                      >  1:552349/1‑140 (MQ=255)

                                                                                                     ccAGCATTACCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGt                            <  2:552349/140‑1 (MQ=255)

                                                                                                             aCCAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTgccagcca                    >  2:328815/1‑140 (MQ=255)

                                                                                                              ccAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTgc                          >  1:509434/1‑133 (MQ=255)

                                                                                                              ccAGCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTgc                          <  2:509434/133‑1 (MQ=255)

                                                                                                                agtaTCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTg                 >  1:826859/4‑140 (MQ=255)

                                                                                                                 gCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTg                           <  2:156695/129‑1 (MQ=255)

                                                                                                                 gCATCGGCATGTCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTg                           >  1:156695/1‑129 (MQ=255)

                                                                                                                            tCGCTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAAttt     >  2:581997/1‑140 (MQ=255)

                                                                                                                               cTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAATCTCTTTTAAAATCGCATGGCCCATTGCCATCGCCAGGGCGCTAGCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGc  <  2:536313/140‑1 (MQ=255)

                                                                                                                               cTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCCCTGGTCGCACAATTTGGc  <  1:539949/140‑1 (MQ=255)

                                                                                                                               cTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGc  <  2:565073/140‑1 (MQ=255)

                                                                                                                               cTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGc  <  2:826859/140‑1 (MQ=255)

                                                                                                                               cTGTAGCGGTGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGc  <  2:862577/140‑1 (MQ=255)

TACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGC  >  NC_000913/1541460‑1541726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A