Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 227 52.0 1764248 99.3% 1751898 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,689,211 A→G E212E (GAA→GAG)  manA → mannose‑6‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,689,2110AG100.0% 137.6 / NA 40E212E (GAA→GAG) manAmannose‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/19);  total (21/19)

GCGATTGCTCACTTTTTACAACAGCCTGATGCCGAACGTTTAAGCGAACTGTTCGCCAGCCTGTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACG  >  NC_000913/1689074‑1689341
                                                                                                                                         |                                                                                                                                  
gCGATTGCTCACTTTTTACAACAGCCTGATGCCGAACGTTTAAGCGAACTGTTCGCCAGCCTGTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGcc                                                                                                                                  >  1:123599/1‑140 (MQ=255)
     tGCTCACTTTTTACAACAGCCTGATGCCGAACGTTTAAGCGAACTGTTCGCCAGCCTGTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGc                                                                                                                             >  2:91415/1‑140 (MQ=255)
       cTCACTTTTTACAACAGCCTGATGCCGAACGTTTAAGCGAACTGTTCGCCAGCCTGTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCaa                                                                                                                           >  1:874990/1‑140 (MQ=255)
                  caacaGCCTGATGCCGAACGTTTAAGCGAACTGTTCGCCAGCCTGTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGttt                                                                                                                <  2:719955/140‑1 (MQ=255)
                    acaGCCCGATGCCGAACGTTTAAGCGAACTGTTCGCCAGCCTGTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTaa                                                                                                              >  2:264676/1‑140 (MQ=255)
                      aGCCTGATGCCGAACGTTTAAGCGAACTGTTCGCCAGCCTGTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAAtt                                                                                                            >  2:69481/1‑140 (MQ=255)
                        ccTGATGCCGAACGTTTAAGCGAACTGTTCGCCAGCCTGTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTc                                                                                                          >  2:376308/1‑140 (MQ=255)
                               ccGAACGTTTAAGCGAACTGTTCGCCAGCCTGTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAAttt                                                                                                   <  2:123599/140‑1 (MQ=255)
                                       ttAAGCGAACTGTTCGCCAGCCTGTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGa                                                                                           >  2:512257/1‑140 (MQ=255)
                                            cGAACTGTTCGCCAGCCTGTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACa                                                                                      <  2:218146/140‑1 (MQ=255)
                                               aCTGTTCGCCAGCCTGTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGCCCCTCGATAGCCAGCAGGGGGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCg                                                                                   >  2:134095/1‑140 (MQ=255)
                                                cTGTTCGCCAGCCTGTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCgg                                                                                  >  2:184839/1‑140 (MQ=255)
                                                           ccTGTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTccc                                                                       >  2:213335/1‑140 (MQ=255)
                                                             tGTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCg                                                                     <  1:90982/140‑1 (MQ=255)
                                                              gTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGc                                                                    <  2:363667/140‑1 (MQ=255)
                                                              gTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGc                                                                    <  2:874990/140‑1 (MQ=255)
                                                               ttGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCt                                                                   <  1:456363/140‑1 (MQ=255)
                                                               ttGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCt                                                                   <  1:376308/140‑1 (MQ=255)
                                                                      tGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTg                                                            >  2:309512/1‑140 (MQ=255)
                                                                         aGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTg                                                               >  2:116213/1‑134 (MQ=255)
                                                                         aGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTg                                                               <  1:116213/134‑1 (MQ=255)
                                                                         aGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAAt                                                         >  1:848073/1‑140 (MQ=255)
                                                                           ggTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAAtgt                                                       >  2:80049/1‑140 (MQ=255)
                                                                                agaaAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGa                                                  >  2:589671/1‑140 (MQ=255)
                                                                                agaaAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGAGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGa                                                  >  2:789551/1‑140 (MQ=255)
                                                                                     aaTCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGGCAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAATTg                                             >  2:820351/1‑140 (MQ=255)
                                                                                     aaTCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAATTg                                             >  2:430301/1‑140 (MQ=255)
                                                                                         ccGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATgtggtg                                                   >  2:193038/1‑130 (MQ=255)
                                                                                         ccGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATgtggtg                                                   <  1:193038/130‑1 (MQ=255)
                                                                                              cgcTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGc                                    >  2:193902/1‑140 (MQ=255)
                                                                                               gcTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCg                                   <  1:820351/140‑1 (MQ=255)
                                                                                               gcTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCACGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCg                                   <  1:512257/140‑1 (MQ=255)
                                                                                                    cGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCg                              <  1:91415/140‑1 (MQ=255)
                                                                                                             aaTCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTg                     <  1:789551/140‑1 (MQ=255)
                                                                                                             aaTCGGCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTg                     <  1:193902/140‑1 (MQ=255)
                                                                                                                 ggCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCg                 >  2:602363/1‑140 (MQ=255)
                                                                                                                  gCCCTCGATAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGc                >  2:412192/1‑140 (MQ=255)
                                                                                                                          tAGCCAGCAGGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAacac        <  1:470822/140‑1 (MQ=255)
                                                                                                                              cagcagGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCa    <  1:475286/140‑1 (MQ=255)
                                                                                                                                gcagGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACg  <  1:134095/140‑1 (MQ=255)
                                                                                                                                gcagGGTGAGCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACg  <  1:412192/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                  
GCGATTGCTCACTTTTTACAACAGCCTGATGCCGAACGTTTAAGCGAACTGTTCGCCAGCCTGTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACG  >  NC_000913/1689074‑1689341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A