Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,714,386	T→C	F4L (TTC→CTC)	gstA →	glutathione S‑transferase GstA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,714,386	0	T	C	100.0%	127.3 / NA	36	F4L (TTC→CTC)	gstA	glutathione S‑transferase GstA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/14); total (22/14)

CCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCT  >  NC_000913/1714261‑1714520

ccGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGt                                                                                                                          <  2:668929/140‑1 (MQ=255)

ccGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCCTTTTTTTTGTGCACCAACTCACCACCATACTTTAAACCACAGACAACAGGAGTTACCGATGAAATTGCTCTACAAACCGGGt                                                                                                                          >  2:616394/1‑140 (MQ=255)

        tAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGctc                                                                                                                  <  2:385891/140‑1 (MQ=255)

             ttCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCg                                                                                                             <  2:481033/140‑1 (MQ=255)

             ttCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCg                                                                                                             <  2:244680/140‑1 (MQ=255)

                 gggAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTc                                                                                                         <  1:258147/140‑1 (MQ=255)

                           tttACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCAcc                                                                                               <  2:342146/140‑1 (MQ=255)

                            ttACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCAcc                                                                                               >  1:305830/1‑138 (MQ=255)

                            ttACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCAcc                                                                                               <  2:305830/138‑1 (MQ=255)

                                    cGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTgag                                                                                      >  1:166680/1‑140 (MQ=255)

                                                     cGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTAc                                                                     >  1:361166/1‑140 (MQ=255)

                                                     cGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTAc                                                                     >  1:513831/1‑140 (MQ=255)

                                                           tttttttGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGt                                                               <  1:371377/140‑1 (MQ=255)

                                                           tttttttGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGt                                                               <  2:361166/140‑1 (MQ=255)

                                                                 tGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAgtgt                                                         >  1:209911/1‑140 (MQ=255)

                                                                          aCGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAAt                                                <  2:513831/140‑1 (MQ=255)

                                                                             cACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGaa                                             >  1:811652/1‑140 (MQ=255)

                                                                             cACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGaa                                             >  1:628807/1‑140 (MQ=255)

                                                                                      aCTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGtct                                    >  1:637985/1‑140 (MQ=255)

                                                                                             aCCACAGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAAc                             >  2:46759/1‑140 (MQ=255)

                                                                                               cacaGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACgg                           >  1:724683/1‑140 (MQ=255)

                                                                                                 caGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTg                         >  2:100182/1‑140 (MQ=255)

                                                                                                 caGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTg                         >  1:658496/1‑140 (MQ=255)

                                                                                                 caGACAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTg                         >  1:652344/1‑140 (MQ=255)

                                                                                                    aCAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACg                      >  2:213648/1‑140 (MQ=255)

                                                                                                     cAAAAGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGa                     <  2:724683/140‑1 (MQ=255)

                                                                                                      aaaaGGAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGAt                    >  1:488121/1‑140 (MQ=255)

                                                                                                           gAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACtt               <  1:447248/140‑1 (MQ=255)

                                                                                                           gAGTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACtt               >  2:447248/1‑140 (MQ=255)

                                                                                                             gTTACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTg             >  2:621916/1‑140 (MQ=255)

                                                                                                              ttACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTg             <  2:862091/139‑1 (MQ=255)

                                                                                                              ttACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTg             >  1:862091/1‑139 (MQ=255)

                                                                                                              ttACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGc            >  1:409112/1‑140 (MQ=255)

                                                                                                              ttACCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGc            >  2:850663/1‑140 (MQ=255)

                                                                                                                aCCGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCg          >  1:224618/1‑140 (MQ=255)

                                                                                                                 ccGATGAAATTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGt         <  1:52114/140‑1 (MQ=255)

                                                                                                                        aaTTGCTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCt  >  2:335348/1‑140 (MQ=255)

CCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCT  >  NC_000913/1714261‑1714520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A