Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,808,148	A→G	D118G (GAC→GGC)	yniA →	putative kinase YniA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,808,148	0	A	G	100.0%	151.2 / NA	43	D118G (GAC→GGC)	yniA	putative kinase YniA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/21); total (22/21)

TGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAAGG  >  NC_000913/1808013‑1808283

tGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGgccaa                                                                                                                                     >  1:695915/1‑140 (MQ=255)

     aaGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACcaca                                                                                                                                <  1:875557/140‑1 (MQ=255)

     aaGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACcaca                                                                                                                                >  2:323257/1‑140 (MQ=255)

           tGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTgg                                                                                                                          >  1:615946/1‑140 (MQ=255)

            gggCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGc                                                                                                                         >  2:84609/1‑140 (MQ=255)

            gggCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGc                                                                                                                         >  2:656361/1‑140 (MQ=255)

             ggCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGcc                                                                                                                        <  1:632508/140‑1 (MQ=255)

              gCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCt                                                                                                                       <  2:284188/140‑1 (MQ=255)

                 gTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGa                                                                                                                    >  2:86908/1‑140 (MQ=255)

                  ttGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGAt                                                                                                                   >  1:630335/1‑140 (MQ=255)

                  ttGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGAt                                                                                                                   >  2:279391/1‑140 (MQ=255)

                  ttGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGAt                                                                                                                   >  1:89250/1‑140 (MQ=255)

                   tGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGAtt                                                                                                                  <  1:211601/140‑1 (MQ=255)

                           aCCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAAc                                                                                                          <  1:244622/140‑1 (MQ=255)

                           aCCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAAc                                                                                                          <  2:200150/140‑1 (MQ=255)

                            ccGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAAcg                                                                                                         >  1:499180/1‑140 (MQ=255)

                                gACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGct                                                                                                     <  2:695915/140‑1 (MQ=255)

                                gACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGct                                                                                                     <  2:615946/140‑1 (MQ=255)

                                     cAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAAc                                                                                                          <  1:364396/130‑1 (MQ=255)

                                     cAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAAc                                                                                                          >  2:364396/1‑130 (MQ=255)

                                     cAGTTTTCTGGAGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCca                                                                                                >  2:860559/1‑140 (MQ=255)

                                          ttCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACt                                                                                           >  2:871271/1‑140 (MQ=255)

                                             tGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCca                                                                                        <  1:142708/140‑1 (MQ=255)

                                             tGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCca                                                                                        <  2:812095/140‑1 (MQ=255)

                                             tGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCca                                                                                        <  1:84609/140‑1 (MQ=255)

                                               gTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCcaca                                                                                      >  2:474041/1‑140 (MQ=255)

                                                          tctcCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCt                                                                           <  1:656361/140‑1 (MQ=255)

                                                            tcCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTgg                                                                         >  2:442781/1‑140 (MQ=255)

                                                               cACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCaa                                                                      <  2:499180/140‑1 (MQ=255)

                                                                 ccTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACg                                                                    >  2:451541/1‑140 (MQ=255)

                                                                           tGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCa                                                          <  1:279391/140‑1 (MQ=255)

                                                                                   cATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGttttt                                                  >  2:352652/1‑140 (MQ=255)

                                                                                     tAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTg                                                <  1:869996/140‑1 (MQ=255)

                                                                                            tttATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGaaca                                         >  2:31676/1‑140 (MQ=255)

                                                                                                     ggTCAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATtgg                                >  1:164350/1‑140 (MQ=255)

                                                                                                        cAGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATtggctg                             <  1:871271/140‑1 (MQ=255)

                                                                                                         aGCAAATTGCGCGTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATtggctgg                            <  1:352652/140‑1 (MQ=255)

                                                                                                                 gcgcGTTTACATCATTGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGa                    >  2:741449/1‑140 (MQ=255)

                                                                                                                  cgcgTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGaa                   <  1:744864/140‑1 (MQ=255)

                                                                                                                     gTTTACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTg                >  1:797329/1‑140 (MQ=255)

                                                                                                                       ttACATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGc              <  1:860559/140‑1 (MQ=255)

                                                                                                                         aCATCAATGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTg            <  1:31676/140‑1 (MQ=255)

                                                                                                                               aTGGAGCGGCCAACCACAATTTGGCCTTGATTTCGATTACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACAGATTGGCTGGCAGTTGGAACTGGCTGCAGaaa      >  2:781602/1‑140 (MQ=255)

                                                                                                                                 ggAGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGaaaaa    >  2:259920/1‑140 (MQ=255)

                                                                                                                                   aGCGGCCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAAgg  >  2:372393/1‑140 (MQ=255)

TGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAAGG  >  NC_000913/1808013‑1808283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A