Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,862,296	T→C	H45R (CAC→CGC)	msrB ←	methionine sulfoxide reductase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,862,296	0	T	C	100.0%	175.9 / NA	49	H45R (CAC→CGC)	msrB	methionine sulfoxide reductase B
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/24); total (25/24)

CATTCCATGTGACAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCAT  >  NC_000913/1862166‑1862429

cATTCCATGTGACAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGc                                                                                                                              >  1:177769/1‑140 (MQ=255)

cATTCCATGTGACAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGc                                                                                                                              >  2:355329/1‑140 (MQ=255)

     cATGTGACAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCa                                                                                                                         >  1:63242/1‑140 (MQ=255)

      aTGTGACAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCAc                                                                                                                        <  2:491637/140‑1 (MQ=255)

      aTGTGACAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCAc                                                                                                                        <  1:265745/140‑1 (MQ=255)

          gACAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCtt                                                                                                                    >  2:847207/1‑140 (MQ=255)

          gACAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCtt                                                                                                                    >  1:767012/1‑140 (MQ=255)

           aCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTg                                                                                                                   <  1:355329/140‑1 (MQ=255)

              aGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTa                                                                                                                <  1:847207/140‑1 (MQ=255)

              aGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTa                                                                                                                <  1:678725/140‑1 (MQ=255)

               gTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTAt                                                                                                               >  2:672607/1‑140 (MQ=255)

                       tataACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCTGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTaag                                                                                                       >  2:548394/1‑139 (MQ=255)

                       aataACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTaaa                                                                                                       <  2:599964/139‑1 (MQ=255)

                          aaCGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGtt                                                                                                                 <  1:588150/127‑1 (MQ=255)

                          aaCGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGtt                                                                                                                 >  2:588150/1‑127 (MQ=255)

                          aaCGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGa                                                                                                    >  1:330123/1‑140 (MQ=255)

                          aaCGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGa                                                                                                    >  2:508303/1‑140 (MQ=255)

                                          aCTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGct                                                                                    <  1:563453/140‑1 (MQ=255)

                                          aCTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGct                                                                                    <  1:278893/140‑1 (MQ=255)

                                          aCTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGct                                                                                    <  2:63242/140‑1 (MQ=255)

                                          aCTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGct                                                                                    <  1:547365/140‑1 (MQ=255)

                                           cTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGctc                                                                                   <  2:767012/140‑1 (MQ=255)

                                               ccGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTc                                                                               <  2:330123/140‑1 (MQ=255)

                                                  gTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCa                                                                            >  1:243585/1‑140 (MQ=255)

                                                    tCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATg                                                                          >  1:709478/1‑140 (MQ=255)

                                                     cGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCAAGa                                                                         >  2:115440/1‑140 (MQ=255)

                                                            aCTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCg                                                                  <  2:174280/140‑1 (MQ=255)

                                                            aCTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCg                                                                  <  2:709478/140‑1 (MQ=255)

                                                            aCTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCg                                                                  >  1:707476/1‑140 (MQ=255)

                                                                ggCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCAc                                                              >  1:330526/1‑140 (MQ=255)

                                                                ggCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCAc                                                              >  1:754329/1‑140 (MQ=255)

                                                                ggCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCAc                                                              >  1:43872/1‑140 (MQ=255)

                                                                          aGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGc                                                    >  2:165527/1‑140 (MQ=255)

                                                                          aGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGc                                                    >  2:58406/1‑140 (MQ=255)

                                                                                     tACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGTGTCACGTAAAACTGCATCTCGGACaa                                         >  1:22904/1‑140 (MQ=255)

                                                                                               gggAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtttttt                                  >  1:547828/1‑137 (MQ=255)

                                                                                               gggAATGAAACAGCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtttttt                                  <  2:547828/137‑1 (MQ=255)

                                                                                                           gCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGcc                   <  2:681298/140‑1 (MQ=255)

                                                                                                           gCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGcc                   <  2:43872/140‑1 (MQ=255)

                                                                                                           gCGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTATCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGcc                   <  1:748158/140‑1 (MQ=255)

                                                                                                            cGGGGCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCg                  >  2:593553/1‑140 (MQ=255)

                                                                                                             ggggCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGa                 <  1:165527/140‑1 (MQ=255)

                                                                                                             ggggCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGa                 <  2:177769/140‑1 (MQ=255)

                                                                                                             ggggCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGa                 <  1:58406/140‑1 (MQ=255)

                                                                                                              gggCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAg                             >  2:737723/1‑127 (MQ=255)

                                                                                                              gggCATCGCAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAg                             <  1:737723/127‑1 (MQ=255)

                                                                                                                gCATCGCAGATCAAACAGCGAGATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAgg              >  1:870633/1‑140 (MQ=255)

                                                                                                                      cAGATCAAACAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTttatt        >  2:269968/1‑140 (MQ=255)

                                                                                                                            aaaCAGCGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCAt  <  2:330526/140‑1 (MQ=255)

CATTCCATGTGACAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCAT  >  NC_000913/1862166‑1862429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A