Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,890,467	A→G	G22G (GGT→GGC)	yeaY ←	Slp family lipoprotein YeaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,890,467	0	A	G	100.0%	87.4 / NA	27	G22G (GGT→GGC)	yeaY	Slp family lipoprotein YeaY
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/6); total (21/6)

TTGCCACCAAAGCGTGCCTCCTGACCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCA  >  NC_000913/1890351‑1890598

ttGCCACCAAAGCGTGCCTCCTGACCAACGTACAGCTGCGGCGCACTCATCCCCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGt                                                                                                              <  1:20280/140‑1 (MQ=255)

ttGCCACCAAAGCGTGCCTCCTGACCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGt                                                                                                              >  1:399748/1‑140 (MQ=255)

  gCCACCAAAGCGTGCCTCCTGACCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTAc                                                                                                            >  1:153282/1‑140 (MQ=255)

         aaGCGTGCCTCCTGACCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAg                                                                                                     >  2:740066/1‑140 (MQ=255)

               gcatcctGACCAACGTACAGCTGCGGCGCCCTCATCCCCCGACCTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGGGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGcc                                                                                               <  2:813771/137‑1 (MQ=255)

                cctcctGACCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGc                                                                                                        <  2:731257/130‑1 (MQ=255)

                cctcctGACCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGc                                                                                                        >  1:731257/1‑130 (MQ=255)

                      gACCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATa                                                                                        >  1:838571/1‑140 (MQ=255)

                         cAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACa                                                                                     <  1:740066/140‑1 (MQ=255)

                           aCGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACAtt                                                                                   <  1:872068/140‑1 (MQ=255)

                                      cggcgCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCg                                                                        >  1:301066/1‑140 (MQ=255)

                                      cggcgCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCg                                                                        >  1:247583/1‑140 (MQ=255)

                                        gcgcACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGcc                                                                      >  1:708615/1‑140 (MQ=255)

                                           cACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATg                                                                   >  1:661980/1‑140 (MQ=255)

                                                        aaCTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCttt                                                      >  2:811554/1‑140 (MQ=255)

                                                        aaCTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCttt                                                      >  1:680526/1‑140 (MQ=255)

                                                        aaCTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCttt                                                      >  1:176970/1‑140 (MQ=255)

                                                        aaCTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGGGCGACTCCttt                                                      >  2:437066/1‑140 (MQ=255)

                                                          cTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCttttt                                                    >  1:796304/1‑140 (MQ=255)

                                                          cTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCttttt                                                    >  1:247829/1‑140 (MQ=255)

                                                              atttttttgGGGCGTGGGACTGCTGCTTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTtct                                                <  2:301066/134‑1 (MQ=255)

                                                              aTCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTtct                                                <  2:176970/140‑1 (MQ=255)

                                                               tCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTtctc                                               >  1:288449/1‑140 (MQ=255)

                                                                       cggcgTGGGCCCTCTGCCTTTAAGGGCGTCGGGCACAGGAACCAGCCCGCTAACAATTAAGCCAACCGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATa                                       <  2:796304/140‑1 (MQ=255)

                                                                            tGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCtt                                  <  1:615690/140‑1 (MQ=255)

                                                                            ggggCCTGCTGCCTTAAATGGCGTCCGGCACAGTGACACAGCCGCACAGCATGAGCGCAACCGTACCTGCCAGTAGGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCtt                                  <  2:399748/139‑1 (MQ=255)

                                                                                    ctgcCTTTAATGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGattca                          >  1:241702/1‑140 (MQ=255)

                                                                                         tttAAGGGCGCCCGGCAAAGTGCCCCAGCCGCTCAGCATTGGCGAAAACGTCCCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCtt                     <  2:829153/140‑1 (MQ=255)

                                                                                            aaTGGCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGc                  >  1:508491/1‑140 (MQ=255)

                                                                                             agggCGCCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAACCGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGcc                 <  2:680526/138‑1 (MQ=255)

                                                                                               ggCGTCCGGCACAGTGACACAGCCGCTCAGCTTTAGCGAAAACGTCCCTGCCAGTATGCCTTTGATATCATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCg               <  2:772608/140‑1 (MQ=255)

                                                                                               ggCGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCg               <  2:838571/140‑1 (MQ=255)

                                                                                                 cGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGa             >  1:829153/1‑140 (MQ=255)

                                                                                                 cGTCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGa             >  1:772608/1‑140 (MQ=255)

                                                                                                   tCCGGCACAGTGACACAGCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAg           >  1:595913/1‑140 (MQ=255)

                                                                                                        cagaggaacacAGCCGCTCGCCATTAGCGCAAACGTCCCTGCCAGTATGCGTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTctct      <  2:708615/133‑1 (MQ=255)

                                                                                                            gTGACACAGCCGCTCAGCATTAGCGCAAACGTCCCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCa  <  1:811554/140‑1 (MQ=255)

TTGCCACCAAAGCGTGCCTCCTGACCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCA  >  NC_000913/1890351‑1890598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A