Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,915,961	T→C	V56V (GTA→GTG)	msrC ←	free methionine‑(R)‑sulfoxide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,915,961	0	T	C	100.0%	162.0 / NA	46	V56V (GTA→GTG)	msrC	free methionine‑(R)‑sulfoxide reductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/24); total (22/24)

CATCACAGGCAATATGCCCGTCAAACACATGAACATCCTCGATACGCTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGTACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCTCCCGCCATCAGCGCGTTAAAGTCG  >  NC_000913/1915825‑1916093

cATCACAGGCAATATGCCCGTCAAACACATGAACATCCTCGATACGCTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCAcc                                                                                                                                   >  2:57189/1‑140 (MQ=255)

 aTCACAGGCAATATGCCCGTCAAACACATGAACATCCTCGATACGCTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCa                                                                                                                                  <  2:232440/140‑1 (MQ=255)

       ggCAATATGCCCGTCAAACACATGAACATCCTCGATACGCTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTAt                                                                                                                            <  2:401178/140‑1 (MQ=255)

        gCAATATGCCCGTCAAACACATGAACATCCTCGATACGCTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATc                                                                                                                           >  2:287011/1‑140 (MQ=255)

        gCAATATGCCCGTCAAACACATGAACATCCTCGATACGCTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATc                                                                                                                           >  1:456454/1‑140 (MQ=255)

        gCAATATGCCCGTCAAACACATGAACATCCTCGATACGCTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATc                                                                                                                           >  1:669146/1‑140 (MQ=255)

             atGCCCGTCAAACACATGAACATCCTCGATACGCTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCt                                                                                                                      >  1:3151/1‑140 (MQ=255)

             atGCCCGTCAAACACATGAACATCCTCGATACGCTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCt                                                                                                                      <  2:351253/140‑1 (MQ=255)

                 ccGTCAAACACATGAACATCCTCGATACGCTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAg                                                                                                                  <  1:556524/140‑1 (MQ=255)

                  cGTCAAACACATGAACATCCTCGATACGCTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGAATCCGGACACAGGCAATTTGGCCCTGAAATGGCCCGAGCACCAGTGTATCGTCCTCAAGc                                                                                                                 >  2:40428/1‑140 (MQ=255)

                    tCAAACACATGAACATCCTCGATACGCTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCaa                                                                                                               >  1:433477/1‑140 (MQ=255)

                            aTGAACATCCTCGATACGCTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAAc                                                                                                       <  2:433477/140‑1 (MQ=255)

                                 cATCCTCGATACGCTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGcc                                                                                                  <  2:343524/140‑1 (MQ=255)

                                    ccTCGATACGCTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAg                                                                                               >  2:524361/1‑140 (MQ=255)

                                      tCGATACGCTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGtt                                                                                             <  1:57189/140‑1 (MQ=255)

                                            cGCTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTc                                                                                       >  2:697843/1‑140 (MQ=255)

                                              cTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAg                                                                                     >  2:282460/1‑140 (MQ=255)

                                               tGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGt                                                                                    <  1:855987/140‑1 (MQ=255)

                                                 cACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTga                                                                                  >  1:638971/1‑140 (MQ=255)

                                                  aCTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTga                                                                                  <  2:363680/139‑1 (MQ=255)

                                                  aCTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTga                                                                                  >  1:363680/1‑139 (MQ=255)

                                                   tttcaTTGTGGGAAAACGCAGTGCCGCAGACGCCGCGCCCGACGGGTATCCGGCCACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTgaga                                                                                <  2:264715/136‑1 (MQ=255)

                                                   cTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTgaga                                                                                <  2:638971/140‑1 (MQ=255)

                                                    ttGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGAc                                                                               <  2:38849/140‑1 (MQ=255)

                                                    ttGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGAc                                                                               <  2:49225/140‑1 (MQ=255)

                                                    ttGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGAc                                                                               <  1:115652/140‑1 (MQ=255)

                                                    ttGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGAc                                                                               <  2:243465/140‑1 (MQ=255)

                                                    ttGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGAc                                                                               <  1:697843/140‑1 (MQ=255)

                                                     tGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACg                                                                              <  2:716683/140‑1 (MQ=255)

                                                      gATTGCGGCCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGc                                                                             <  2:669146/140‑1 (MQ=255)

                                                       aTTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCt                                                                            <  1:282460/140‑1 (MQ=255)

                                                         tGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCa                                                                          >  1:54651/1‑140 (MQ=255)

                                                           cGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTg                                                                                   <  2:11187/129‑1 (MQ=255)

                                                           cGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTg                                                                                   >  1:11187/1‑129 (MQ=255)

                                                           cGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGAc                                                                               >  2:187874/1‑133 (MQ=255)

                                                           cGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGAc                                                                               >  2:9398/1‑133 (MQ=255)

                                                           cGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGAc                                                                               <  1:187874/133‑1 (MQ=255)

                                                           cGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGAc                                                                               <  1:9398/133‑1 (MQ=255)

                                                              gCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATaa                                                                     <  1:287011/140‑1 (MQ=255)

                                                                  ccGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGAc                                                                               <  1:169589/126‑1 (MQ=255)

                                                                  ccGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGAc                                                                               >  2:169589/1‑126 (MQ=255)

                                                                                    cgcgCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGc                                               >  2:423274/1‑140 (MQ=255)

                                                                                                    ccGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTgg                               <  2:456454/140‑1 (MQ=255)

                                                                                                          acaGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTtctc                         >  2:178853/1‑140 (MQ=255)

                                                                                                           caGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCTcc                        >  2:631593/1‑140 (MQ=255)

                                                                                                            aGGCAATTTTGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCTccc                       >  2:367968/1‑140 (MQ=255)

                                                                                                                     tGCCCTGAAATGGTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCTCCCGCCATCAgc              >  2:371590/1‑140 (MQ=255)

                                                                                                                                 gTCCGAGCACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCTCCCGCCATCAGCGCGTTAAAGTcg  <  1:367968/140‑1 (MQ=255)

CATCACAGGCAATATGCCCGTCAAACACATGAACATCCTCGATACGCTGCACTTGATTGCGGGCAACCGCAGTGCCGCACACGCCGCGCCCGACGGGTATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGTACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCTCCCGCCATCAGCGCGTTAAAGTCG  >  NC_000913/1915825‑1916093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A