Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,945,195	G→A	A54A (GCC→GCT)	ruvB ←	Holliday junction branch migration complex subunit RuvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,945,195	0	G	A	100.0%	137.2 / NA	39	A54A (GCC→GCT)	ruvB	Holliday junction branch migration complex subunit RuvB
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/21); total (18/21)

TCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCA  >  NC_000913/1945059‑1945330

tCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCa                                                                                                                                      <  1:355495/140‑1 (MQ=255)

 cGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCAt                                                                                                                                     >  2:252364/1‑140 (MQ=255)

   cAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATcg                                                                                                                                   >  1:485436/1‑140 (MQ=255)

   cAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATcg                                                                                                                                   >  2:724684/1‑140 (MQ=255)

      ccAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATcgccg                                                                                                                                <  2:442444/140‑1 (MQ=255)

        aaaTCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCcgcg                                                                                                                              >  1:394083/1‑140 (MQ=255)

                  ccTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCgct                                                                                                                    >  2:110611/1‑140 (MQ=255)

                            cACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTtgatga                                                                                                          <  1:724684/140‑1 (MQ=255)

                            cACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTtgatga                                                                                                          <  1:451130/140‑1 (MQ=255)

                            cACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTtgatga                                                                                                          <  2:663071/140‑1 (MQ=255)

                                ggACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAAt                                                                                                      <  2:749491/140‑1 (MQ=255)

                                 gACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAAtc                                                                                                     >  2:593654/1‑140 (MQ=255)

                                 gACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAAtc                                                                                                     >  1:417959/1‑140 (MQ=255)

                                 gACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAAtc                                                                                                     >  1:173768/1‑140 (MQ=255)

                                 gACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAAtc                                                                                                     >  1:123887/1‑140 (MQ=255)

                                      gAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCAt                                                                                                <  1:196016/140‑1 (MQ=255)

                                                       aaCGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCg                                                                               >  1:133518/1‑140 (MQ=255)

                                                        aCGCCCATTTCATTGGCGACAATGTTGGCGAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCgg                                                                              <  2:123887/140‑1 (MQ=255)

                                                          gCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCt                                                                            <  1:283312/140‑1 (MQ=255)

                                                          gCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCt                                                                            <  2:485436/140‑1 (MQ=255)

                                                             cATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGAc                                                                         <  2:394083/140‑1 (MQ=255)

                                                                  cATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACa                                                                    <  2:173768/140‑1 (MQ=255)

                                                                          aCAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTc                                                            >  1:568906/1‑140 (MQ=255)

                                                                            aaTGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCa                                                          >  1:249194/1‑140 (MQ=255)

                                                                            aaTGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCa                                                          <  1:403377/140‑1 (MQ=255)

                                                                                 tGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAAt                                                     <  2:326681/140‑1 (MQ=255)

                                                                                  ggCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAAtt                                                    >  1:805551/1‑140 (MQ=255)

                                                                                      aGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTggg                                                >  1:178468/1‑140 (MQ=255)

                                                                                        cGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGc                                              >  1:409620/1‑140 (MQ=255)

                                                                                        cGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGc                                              <  2:133518/140‑1 (MQ=255)

                                                                                            gTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAAt                                          <  1:593654/140‑1 (MQ=255)

                                                                                                tACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGgcg                                      <  2:417959/140‑1 (MQ=255)

                                                                                                                 ccAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTc                     >  2:766630/1‑140 (MQ=255)

                                                                                                                 ccAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTc                     >  2:122811/1‑140 (MQ=255)

                                                                                                                 ccAAAAATCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTc                     >  2:830333/1‑140 (MQ=255)

                                                                                                                     aaaTCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGc                 <  2:179601/140‑1 (MQ=255)

                                                                                                                      aaTCAACAAATGATCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCa                <  2:342044/140‑1 (MQ=255)

                                                                                                                                  aTCGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCgg    <  2:178468/140‑1 (MQ=255)

                                                                                                                                    cGAGAGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCa  <  1:819333/140‑1 (MQ=255)

TCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCA  >  NC_000913/1945059‑1945330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A