Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,946,665	A→C	T139P (ACG→CCG)	yebB →	putative papain‑like amidase YebB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,946,665	0	A	C	100.0%	141.2 / NA	40	T139P (ACG→CCG)	yebB	putative papain‑like amidase YebB
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (25/15); total (25/15)

GAACAGGTTCCCTCCCGGCTACGCAAACTTTACCACACCGGTTTTAAATACGAATCTTCGCGCCAGTTCTGTTCAAAATTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGACGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTT  >  NC_000913/1946533‑1946792

gAACAGGTTCCCTCCCGGCTACGCAAACTTTACCACACCGGTTTTAAATACGAATCTTCGCGCCAGTTCTGTTCAAAATTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGCCGTTTgg                                                                                                                          <  1:54170/140‑1 (MQ=255)

    aGGTTCCCTCCCGGCTACGCAAACTTTACCACACCGGTTTTAAATGCGAATCTTCGCGCCAGTTCTGTTCAAAATTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGaa                                                                                                                      <  1:412225/140‑1 (MQ=255)

     ggTTCCCTCCCGGCTACGCAAACTTTACCACACCGGTTTTAAATACGAATCTTCGCGCCAGTTCTGTTCAAAATTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAAt                                                                                                                     <  2:528876/140‑1 (MQ=255)

     ggTTCCCTCCCGGCTACGCAAACTTTACCACACCGGTTTTAAATACGAATCTTCGCGCCAGTTCTGTTCAAAATTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAAt                                                                                                                     >  2:175497/1‑140 (MQ=255)

      gTTCCCTCCCGGCTACGCAAACTTTACCACACCGGTTTTAAATACGAATCTTCGCGCCAGTTCTGTTCAAAATTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAAtt                                                                                                                    >  2:48646/1‑140 (MQ=255)

        tccctcccGGCTACGCAAACTTTACCACACCGGTTTTAAATACGAATCTTCGCGCCAGTTCTGTTCAAAATTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGCCGt                                                                                                                              <  2:323135/128‑1 (MQ=255)

        tccctcccGGCTACGCAAACTTTACCACACCGGTTTTAAATACGAATCTTCGCGCCAGTTCTGTTCAAAATTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGCCGt                                                                                                                              >  1:323135/1‑128 (MQ=255)

        tccctcccGGCTACGCAAACTTTACCACACCGGTTTTAAATACGAATCTTCGCGCCAGTTCTGTTCAAAATATGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGt                                                                                                                  >  2:616414/1‑140 (MQ=255)

                ggCTACGCAAACTTTACCACACCGGTTTTAAATACGAATCTTCGCGCCAGTTCTGTTCAAAATTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGTTAAATAGc                                                                                                          >  2:31419/1‑140 (MQ=255)

                          aCTTTACCACACCGGTTTTAAATACGAATCTTCGCGCCAGTTCTGTTCAAAATTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATg                                                                                                <  1:616414/140‑1 (MQ=255)

                             ttACCACACCGGTTTTAAATACGAATCTTCGCGCCAGTTCTGTTCAAAATTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCaa                                                                                             <  1:31419/140‑1 (MQ=255)

                                              aaTACGAATCTTCGCGCCAGTTCTGTTCAAAATTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGaaa                                                                            >  2:682540/1‑140 (MQ=255)

                                                        ttCGCGCCAGTTCTGTTCAAAATTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTcc                                                                  >  2:1509/1‑140 (MQ=255)

                                                                 gTTCTGTTCAAAATTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTa                                                         >  1:443638/1‑140 (MQ=255)

                                                                       ttCAAAATTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGCAGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGt                                                   <  2:435922/140‑1 (MQ=255)

                                                                          aaaaTTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTggg                                                <  1:432843/140‑1 (MQ=255)

                                                                           aaaTTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGa                                               <  1:208934/140‑1 (MQ=255)

                                                                           aaaTTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGa                                               <  2:700792/140‑1 (MQ=255)

                                                                                                   gaagCGCTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGccagccag                       >  1:602457/1‑140 (MQ=255)

                                                                                                     agCGCTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGtt                     >  2:526223/1‑140 (MQ=255)

                                                                                                      gcgcTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGttt                    >  2:98553/1‑140 (MQ=255)

                                                                                                      gcgcTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGttt                    >  1:806220/1‑140 (MQ=255)

                                                                                                       cgcTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTtg                   >  1:166350/1‑140 (MQ=255)

                                                                                                       cgcTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTtg                   >  2:200992/1‑140 (MQ=255)

                                                                                                         cTATGTATTCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTtgtg                 <  2:602457/140‑1 (MQ=255)

                                                                                                                 tCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCatcatc         >  2:185847/1‑140 (MQ=255)

                                                                                                                 tCCGGTGGGTGAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCatcatc         >  2:830174/1‑140 (MQ=255)

                                                                                                                      tgggtgAAATAGAGCCGTTTGGAGTATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGt    >  2:409524/1‑140 (MQ=255)

                                                                                                                      tgggtgAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGt    >  2:45821/1‑140 (MQ=255)

                                                                                                                      tgggtgAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGt    >  2:484495/1‑140 (MQ=255)

                                                                                                                      tgggtgAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGt    <  2:653292/140‑1 (MQ=255)

                                                                                                                      tgggtgAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGt    <  1:185847/140‑1 (MQ=255)

                                                                                                                      tgggtgAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGt    >  2:69745/1‑140 (MQ=255)

                                                                                                                       gggtgAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGtt   <  2:735826/140‑1 (MQ=255)

                                                                                                                       gggtgAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGtt   <  2:672768/140‑1 (MQ=255)

                                                                                                                        ggtgAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGttt  >  1:237831/1‑140 (MQ=255)

                                                                                                                        ggtgAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGttt  >  1:235759/1‑140 (MQ=255)

                                                                                                                        ggtgAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGttt  >  2:641595/1‑140 (MQ=255)

                                                                                                                        ggtgAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGttt  >  1:432191/1‑140 (MQ=255)

                                                                                                                        ggtgAAATAGAGCCGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGttt  >  2:425994/1‑140 (MQ=255)

GAACAGGTTCCCTCCCGGCTACGCAAACTTTACCACACCGGTTTTAAATACGAATCTTCGCGCCAGTTCTGTTCAAAATTTGTTTTTGATATTTATAAAGAAGCGCTATGTATTCCGGTGGGTGAAATAGAGACGTTTGGAGAATTGTTAAATAGCAATCCGAATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTT  >  NC_000913/1946533‑1946792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A