Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,997,416	C→T	G133G (GGG→GGA)	tcyN ←	cystine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,997,416	0	C	T	100.0%	144.5 / NA	41	G133G (GGG→GGA)	tcyN	cystine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (23/18); total (23/18)

CTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGCCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATGTTCTCCAGCACCGTACGATGCGGAAACAAATTAAAGTTCTGGAAGACAAAC  >  NC_000913/1997281‑1997551

ctctGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCaa                                                                                                                                     <  2:535991/140‑1 (MQ=255)

     gATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACtttt                                                                                                                                >  1:486569/1‑140 (MQ=255)

         cAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCca                                                                                                                            >  2:335306/1‑140 (MQ=255)

          aGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCcag                                                                                                                           >  1:564497/1‑140 (MQ=255)

             gcTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCcagcag                                                                                                                        >  2:216729/1‑140 (MQ=255)

                    ttGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGa                                                                                                                 <  1:335306/140‑1 (MQ=255)

                      ggCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAgc                                                                                                               >  1:426303/1‑140 (MQ=255)

                           gTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAgcgcgcg                                                                                                          >  2:553233/1‑140 (MQ=255)

                             caaacaaaATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGcc                                                                                                        >  2:23833/1‑140 (MQ=255)

                              aaacaaaATCCCCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGTTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCg                                                                                                       <  2:182399/140‑1 (MQ=255)

                              aaacaaaATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCg                                                                                                       <  1:23833/140‑1 (MQ=255)

                                          cTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGGCGTGGCCTcttctt                                                                                           >  2:544881/1‑140 (MQ=255)

                                                aCGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGtt                                                                                     <  1:544881/140‑1 (MQ=255)

                                                aCGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGtt                                                                                     <  1:553233/140‑1 (MQ=255)

                                                 cGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCttt                                                                                          <  2:256237/134‑1 (MQ=255)

                                                 cGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCttt                                                                                          >  1:256237/1‑134 (MQ=255)

                                                                  cgcAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCg                                                                   >  2:17022/1‑140 (MQ=255)

                                                                     aaTCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGTTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGcc                                                                <  2:98696/140‑1 (MQ=255)

                                                                     aaTCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGcc                                                                <  2:486569/140‑1 (MQ=255)

                                                                       tCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCt                                                              >  1:491885/1‑140 (MQ=255)

                                                                                gctgctTTTGACCGCCAGACAAACGACGTGGATTGCTGGTTTCTTGTCCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATg                                                     <  2:261664/140‑1 (MQ=255)

                                                                                gctgctGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATg                                                     <  1:17022/140‑1 (MQ=255)

                                                                                gctgctGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATg                                                     <  1:522813/140‑1 (MQ=255)

                                                                                  tgctgTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCTTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATGtt                                                   >  1:524339/1‑140 (MQ=255)

                                                                                  tgctgTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATGtt                                                   >  2:846467/1‑140 (MQ=255)

                                                                                  tgctgTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATGtt                                                   >  2:151544/1‑140 (MQ=255)

                                                                                     tgTTGACCGCCAGACAAACGACGTGGATAGCTGTTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATGTtctc                                                <  2:273160/140‑1 (MQ=255)

                                                                                       ttGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATGTTCTCCa                                              <  2:564497/140‑1 (MQ=255)

                                                                                       ttGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATGTTCTCCa                                              <  1:303663/140‑1 (MQ=255)

                                                                                        tGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATGTTCTCCAg                                             <  2:491885/140‑1 (MQ=255)

                                                                                          aCCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATGTTCTCCAGCa                                           >  2:54989/1‑140 (MQ=255)

                                                                                          aCCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATGTTCTCCAGCa                                           >  2:437740/1‑140 (MQ=255)

                                                                                            cGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATGTTCTCCAGCAcc                                         >  1:828142/1‑140 (MQ=255)

                                                                                              ccAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATg                                                     >  2:566076/1‑126 (MQ=255)

                                                                                              ccAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATg                                                     <  1:566076/126‑1 (MQ=255)

                                                                                                                   cTGGTTTCTTTACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATGTTCTCCAGCACCGTACGATGCGGAAACAAATTaaa                  >  1:536187/1‑140 (MQ=255)

                                                                                                                            ttcccTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATGTTCTCCAGCACCGTACGATGCGGAAACAAATTAAAGTTCTGGaa         <  2:324041/137‑1 (MQ=255)

                                                                                                                             tACCTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATGTTCTCCAGCACCGTACGATGCGGAAACAAATTAAAGTTCTGGAAg        >  1:314812/1‑140 (MQ=255)

                                                                                                                                cTGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATGTTCTCCAGCACCGTACGATGCGGAAACAAATTAAAGTTCTGGAAGACa     >  2:277236/1‑140 (MQ=255)

                                                                                                                                 tGCCAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATGTTCTCCAGCACCGTACGATGCGGAAACAAATTAAAGTTCTGGAAGACaa    >  1:270084/1‑140 (MQ=255)

                                                                                                                                   ccAGTCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATGTTCTCCAACACCGTACGATGCGGAAACAAATTAAAGTTCTGGAAGACAAAc  >  1:77360/1‑140 (MQ=255)

CTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAGCCCAACTTTTGCCAGCAGCTCGCGAGCGCGCGCCGTGGCCTCTTCTTTCGGTTCACCTTTGACGATCACCGGCCCTTCAATAATGTTCTCCAGCACCGTACGATGCGGAAACAAATTAAAGTTCTGGAAGACAAAC  >  NC_000913/1997281‑1997551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A