Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,394,460	T→C	N196S (AAC→AGC)	nuoL ←	NADH:quinone oxidoreductase subunit L

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,394,460	0	T	C	100.0%	155.3 / NA	44	N196S (AAC→AGC)	nuoL	NADH:quinone oxidoreductase subunit L
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/22); total (22/22)

GTCGGCAAGCCATGTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAA  >  NC_000913/2394324‑2394576

gTCGGCAAGCCATGTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCa                                                                                                                   <  2:607351/140‑1 (MQ=255)

  cGGCAAGCCATGTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAgg                                                                                                                 <  1:745204/140‑1 (MQ=255)

   ggCAAGCCATGTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCa                                                                                                                   >  2:452267/1‑137 (MQ=255)

   ggCAAGCCATGTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCa                                                                                                                   <  1:452267/137‑1 (MQ=255)

             gTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTc                                                                                                      >  2:23635/1‑140 (MQ=255)

              tCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCg                                                                                                     >  1:291957/1‑140 (MQ=255)

                  cAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGt                                                                                                 <  1:290980/140‑1 (MQ=255)

                        cAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCAAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGt                                                                                                        >  2:563012/1‑127 (MQ=255)

                        cAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCAAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGt                                                                                                        <  1:563012/127‑1 (MQ=255)

                            tGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGaa                                                                                       <  2:393201/140‑1 (MQ=255)

                              cgcAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACa                                                                                     >  2:247382/1‑140 (MQ=255)

                               gcAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGCGCCCAGTTCGTTGTAAAGAATGAACAg                                                                                    >  1:706422/1‑140 (MQ=255)

                                             cgcaccgcCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCg                                                                                >  1:439669/1‑130 (MQ=255)

                                             cgcaccgcCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCg                                                                                <  2:439669/130‑1 (MQ=255)

                                             cgcaccgcCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGa                                                                      >  2:594765/1‑140 (MQ=255)

                                               caccgcCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAac                                                                    <  1:376259/140‑1 (MQ=255)

                                               caccgcCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAac                                                                    >  1:377862/1‑140 (MQ=255)

                                                           gcaTCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCcaca                                                        <  2:706422/140‑1 (MQ=255)

                                                            caTCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCacac                                                       <  1:444280/140‑1 (MQ=255)

                                                                           caTCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACg                                        <  2:519236/140‑1 (MQ=255)

                                                                                  aTGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAGGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCAcgacga                                           <  2:375933/130‑1 (MQ=255)

                                                                                  aTGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAGGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCAcgacga                                           >  1:375933/1‑130 (MQ=255)

                                                                                  aTGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCAcgacga                                           <  2:851442/130‑1 (MQ=255)

                                                                                  aTGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCAcgacga                                           >  1:851442/1‑130 (MQ=255)

                                                                                  aTGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCAt                                 >  1:380254/1‑140 (MQ=255)

                                                                                      tATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGc                                       >  2:815264/1‑130 (MQ=255)

                                                                                      tATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGc                                       <  1:815264/130‑1 (MQ=255)

                                                                                      tATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGc                                       >  2:622159/1‑130 (MQ=255)

                                                                                      tATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGc                                       <  1:622159/130‑1 (MQ=255)

                                                                                       aTTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCg                            <  1:452016/140‑1 (MQ=255)

                                                                                       aTTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCg                            <  1:541752/140‑1 (MQ=255)

                                                                                          gCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTg                         >  2:725065/1‑140 (MQ=255)

                                                                                          gCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTg                         <  1:718999/140‑1 (MQ=255)

                                                                                              tCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGcc                     >  1:258072/1‑140 (MQ=255)

                                                                                              tCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGcc                     >  2:70282/1‑140 (MQ=255)

                                                                                               cAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCa                    >  1:273878/1‑140 (MQ=255)

                                                                                               cAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCa                    >  1:161736/1‑140 (MQ=255)

                                                                                                aGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCAt                   >  1:859591/1‑140 (MQ=255)

                                                                                                    aaGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCAttctt               <  2:466184/140‑1 (MQ=255)

                                                                                                         cgcTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGAt          <  1:725065/140‑1 (MQ=255)

                                                                                                         cgcTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGAt          <  2:380254/140‑1 (MQ=255)

                                                                                                         cgcTGGTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGAt          <  2:377862/140‑1 (MQ=255)

                                                                                                              gTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGAt          >  1:746516/1‑135 (MQ=255)

                                                                                                              gTGCCAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGAt          <  2:746516/135‑1 (MQ=255)

                                                                                                                 ccAGTTCCACCATTTCGCGGAAGCTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTaa  >  2:455862/1‑140 (MQ=255)

GTCGGCAAGCCATGTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAA  >  NC_000913/2394324‑2394576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A